Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study without restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2007
Report date:
2007

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
2-cyano-2-methyl-propanoic acid methyl ester
EC Number:
615-749-4
Cas Number:
72291-30-6
Molecular formula:
C6 H9 N O2
IUPAC Name:
2-cyano-2-methyl-propanoic acid methyl ester
Details on test material:
- Name of test substance: 2-Cyano-2-methyl-propanoic acid-methyl ester
- Batch No.: 16/48/7
- Date of manufacture: 4 Feb 2004
- Purity: 99.8%

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Strain: STRAUS
- Source: The clone of Daphnia magna STRAUS 1820 used in the study was originally obtained from the Institut National de Recherche Chimique Appliquée, France, in 1978. From this date on this clone was cultured and bred continuously in the Laboratory of Ecotoxicology of the Experimental Toxicology and Ecology of the BASF Aktiengesellschaft, Ludwigshafen, Germany.
- Age at study initiation (mean and range, SD): 2 - < 24 h (starting with the 3rd breed of parent animals)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
2.46 [mmol/L]
pH:
7.7-8.3
Dissolved oxygen:
8.4-9.4 mg/L
Nominal and measured concentrations:
0, 12.5, 25, 50, 100 mg/L based on test substance (nominal)
Details on test conditions:
TEST SYSTEM
- Test vessel: Test tubes (glass) sealed with gas unpermeable teflone caps (nominal volume 23 mL)
- Type (delete if not applicable): closed
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 light:dark
Reference substance (positive control):
yes
Remarks:
potassium dichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: Nominal concentration verified analytically
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: Nominal concentration verified analytically
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: Nominal concentration verified analytically

Applicant's summary and conclusion

Validity criteria fulfilled:
yes