Cyclohexyl phenyl ketone

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study without detailed documentation.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

1992 followed

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

1992 followed

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Automatically generated during migration to IUCLID 6, no data available

IUPAC Name:

Automatically generated during migration to IUCLID 6, no data available

Details on test material:

- Name of test material (as cited in study report): TKA 40143
- Physical state: solid
- Analytical purity: 88.8%
- Lot/batch No.: PNr. 5021
- Expiration date of the lot/batch: December 01, 1997
- Storage condition of test material: At room temperature in the dark

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

A stock solution was prepared at a nominal concentration of 100 mg/L: 503.4 mg of test substance was dispersed in 5 liters of test medium using ultra-sonic treatment and intensive stirring. Temperature of the medium had increased to 35°C during preparation. The final stock solution was a homogeneous suspension. This stock was used to prepare the different test concentrations.

Test organisms

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: Carp
- Source: Zodiac, proefacc, "De Haar Vissen", L.U. Wageningen, the Netherlands.
- Length at study initiation (length definition, mean, range and SD): 2.75 ± 0.34 cm.
- Weight at study initiation (mean and range, SD): 0.76 ± 0.21 g.

ACCLIMATION
In the batch of fish used for the test, no mortality occurred during the seven days prior to the start of the test.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

No post exposure observation period.

Test conditions

Hardness:

218 mg CaCO3 / L

Test temperature:

20.3 - 20.6 ºC

pH:

Controls: 8.0 - 7.8
10 mg/L: 7.6 (t = 96h)
100 mg/L: 7.8 (t = 0h & t = 2h)

Dissolved oxygen:

Oxygen concentration was not measured. The tanks were aerated.

Nominal and measured concentrations:

Nominal: 0.1, 1, 10, and 100 mg/L

Details on test conditions:

TEST SYSTEM
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.95 g fish/L, 5 fish per 4 L of medium

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO-medium

EFFECT PARAMETERS MEASURED: mortality at 24, 48, 72 and 96 hours

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

11 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL 5.4 - 51 mg/L

Details on results:

No mortality observed in control.

At 100 mg/L, all fish died within 2.25 hours following the start of the exposure.

At 10 mg/L after 24 hours, the fish were discoloured.

At 10 mg/L after 48, 72, and 96 hours, the surviving fish were discoloured and hpoactive compared to the controls.