Methyl 3-methoxypropionate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1989-1990

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method B.1 (Acute Toxicity (Oral))

GLP compliance:

yes (incl. QA statement)

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Methyl 3-methoxypropionate

EC Number:

223-358-1

EC Name:

Methyl 3-methoxypropionate

Cas Number:

3852-09-3

Molecular formula:

C5H10O3

IUPAC Name:

methyl 3-methoxypropanoate

Details on test material:

- clear colourless liquid

Test animals

Species:

rat

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Charles River UK Limited, Margate, Kent, England
- Age at study initiation: 4-6 weeks
- Weight at study initiation: 101-150g (prior to dosing (day 1))
- Fasting period before study: access to food only was prevented overnight prior to and approximately 4 hrs after dosing
- Diet: ad libitum
- Water: ad libitum
- Acclimation period: minimum 7 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21-23 (mean daily minimum-maximum)
- Humidity (%): 57 (mean daily)
- Air changes (per hr): approximately 15
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Doses:

2.0, 3.2, 5.0 g/kg bw

No. of animals per sex per dose:

5

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: animals were observed soon after dosing and at frequent intervals for the remainder of day 1, on subsuquent days the animals were observed once in the morning and again at the end of the experimental day; individual bodyweights on days 1 (day of dosing), 8, 15 and death
- Necropsy of survivors performed: yes

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LD50

Effect level:

4 200 mg/kg bw

Based on:

test mat.

95% CL:

3 600 - 5 000

Effect levels 2

Sex:

male

Dose descriptor:

LD50

Effect level:

4 900 mg/kg bw

Based on:

test mat.

95% CL:

3 900 - 6 100

Effect levels 3

Sex:

female

Dose descriptor:

LD50

Effect level:

3 700 mg/kg bw

Based on:

test mat.

95% CL:

3 100 - 4 400

Mortality:

There were deaths among male and female rats following a single oral dose at 5.0 g/kg, and one female rat died at a dose of 3.2 g/kg. Deaths occurred within 1 h of dosing until day 2 in male and female rats.

Clinical signs:

other: Pilo-erection was observed in all rats within 5 min of dosing. This was accompanied by increased salivation in all rats dosed at 2.0 and 3.2 g/kg bw, and in a majority of rats dosed at 5.0 g/kg bw. Pilo-erection persisted throughout the remainder of day 1

Gross pathology:

Autopsy of the rats that died during the study revealed slightly congested lungs in one male and one female rat. No macroscopic abnormalities were seen in any of the other rats that died.
Terminal autopsy findings of surviving rats were normal.

Applicant's summary and conclusion

Conclusions:

The acute median lethal oral dose (LD50) and their 95% CL limits to female/male rats combined is: 4.2 (3.6-5.0) g/kg bodyweight.