Methyl 3-methoxypropionate

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2015-2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Recent GLP study according to OECD guideline

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.3 (Algal Inhibition test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Methyl 3-methoxypropionate

EC Number:

223-358-1

EC Name:

Methyl 3-methoxypropionate

Cas Number:

3852-09-3

Molecular formula:

C5H10O3

IUPAC Name:

methyl 3-methoxypropanoate

Details on test material:

- Lot/batch No.: M50623Y02
- Storage condition of test material: at room temperature

Sampling and analysis

Analytical monitoring:

yes

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Strain: 61.81 SAG
- Source: SAG: Collection of Algal Cultures, Inst. Plant Physiology, University of Göttingen, Germany

ACCLIMATION
- Culturing media and conditions (same as test or not): The pre-culture is intended to give a quantity of algae suitable for the inoculation of test cultures. The pre-culture will be prepared with the OECD algal growth medium, incubated under the same conditions as the test and used when still growing exponentially, normally after an incubation period of about three days. When the algal cultures contain deformed or abnormal cells, they will be discarded.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Test conditions

Test temperature:

22.8-23.0

pH:

7.59-8.15

Nominal and measured concentrations:

nominal: 100 mg/l
measured:
- at the start: 97.6 mg/l
- at the end: 88.4 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: 250 mL Erlenmeyer flask
- Fill volume: 100 mL
- Aeration: air-permeable stopper
- Initial cells density: approximately 10000 cells/ml
- Control end cells density: 695000 cells/ml
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6

GROWTH MEDIUM
- Standard medium used: yes

TEST MEDIUM / WATER PARAMETERS
- Dilution water: reconstituted algal growth medium (OECD medium, according to OECD 201)

OTHER TEST CONDITIONS
- Light intensity and quality: about 8145.2 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: counting chamber

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0.1, 1, 10, 100 mg/l
- Results used to determine the conditions for the definitive study: no toxic response observed during range-finding test

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Basis for effect:

biomass

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Basis for effect:

other: yield

Effect concentrations 4

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Effect concentrations 5

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Results with reference substance (positive control):

For the evaluation of the quality of the algae and validation of the experimental
conditions, Potassium dichromate is tested at least twice a year to demonstrate
satisfactory test conditions.

- Results with reference substance valid?
ErC50 (72h): 0.84 mg/l
EbC50 (72h): 0.59 mg/l
EyC50 (72h): 0.55 mg/l
These values are within the range of laboratory ring test data (ISO 8692)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Executive summary:

The effect of methyl 3 -methoxypropionate was assessed on algal growth using the unicellular green alga Pseudokirchneriella subcapitata. Because a toxic response was not observed in the preliminary range-finding test, a limit test was carried out using only one test concentration (100 mg/l, nominal) and one control group.

The NOEC was determined to be 100 mg/l.