5-[(4-chloro-2-nitrophenyl)azo]-1-ethyl-1,2-dihydro-6-hydroxy-4-methyl-2-oxonicotinonitrile

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Start of experiment: 8 January 1990; End of experiment: 22 January 1990

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

Comparable with internationally accepted guideline - Internal CIBA method A-11

Deviations:

not specified

GLP compliance:

no

Specific details on test material used for the study:

Name: FAT 36091/F
Purity: 39.6 %

Analytical monitoring:

no

Vehicle:

yes

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

23 °C

pH:

8.2-8.4

Nominal and measured concentrations:

Nominal concentrations: 100 / 300 and 1000 mg/L

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- At 100 mg/L: all 10 fishes where alive at 48 and 96 hours of exposure time.
- At 300 mg/L: all 10 fishes where alive at 48 and 96 hours of exposure time.
- At 1000 mg/L: all 10 fishes where alive at 48 and 8 fishes survived at 96 hours of exposure time.

Validity criteria fulfilled:

yes

Conclusions:

The LC50 of FAT 36091/F in this fish acute toxicity test was determined to be greater than 1000 mg/L.

Executive summary:

The acute fish toxicity test was performed according to equivalent or similar guideline as OECD 203. 10 Zebra fish were used in this test at each dose level.

Fishes were tested at these differents follwing concentrations: 100, 300 and 1000 mg/L.

- At 100 mg/L: all 10 fishes where alive at 48 and 96 hours of exposure time.

- At 300 mg/L:  all 10 fishes where alive at 48 and 96 hours of exposure time.

- At 1000 mg/L: all 10 fishes where alive at 48 and 8 fishes survived at 96 hours of exposure time.

Based on the findings of the study; following conculsion was drawn;

After 48 hours exposure time:

LC0 >= 1000 mg/L

LC50 > 1000 mg/L

LC100 >1000 mg/L

After 96 hours exposure time:

LC0 >300 mg/L

LC50 > 1000 mg/L

LC100 >1000 mg/L

The LC50 of FAT 36091/F in this fish acute toxicity test was determined to be greater than 1000 mg/L.

Description of key information

Several studies were carried out to determine the acute fish toxicity. The LC50 in the key acute toxicity study in fish (Batch F), performed on zebra fish was determined to be greater than 1000 mg/l (48 and 96 hours exposure time).

Supporting studies were perfomredfor all other batches and the LC50 was found to be:

- For batch A: LC50 >450 mg/L (performed on Trout, after 48 hours exposure time).

- For batch B: LC50 is 125 mg/L (performed on Trout, after 48 hours exposure time).

- For batch C: LC50 >1000 mg/L (performed on Trout, after 48 hours exposure time using Tween 80 and Acetone as vehicle).

- For batch C: LC50 >1000 mg/L (performed on Red Killi fish, after 48 hours exposure time).

- For batch D: LC50 is 550 mg/L (performed on Trout, after 48 hours exposure time).

- For batch E: LC50 is 559.49 mg/L and 462.39 mg/L (performed on Zebra fish, after 48 hours and 96 hours exposure time respectively).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1 000 mg/L