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Diss Factsheets

Ecotoxicological information

Long-term toxicity to fish

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
fish early-life stage toxicity
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2009
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 212 (Fish, Short-term Toxicity Test on Embryo and Sac-Fry Stages)
Deviations:
yes
Remarks:
see free text
Principles of method if other than guideline:
Combination of OECD 203 (Adult Zebrafish Toxicity Test), Zebrafish Micronucleus Assay, Exposure of Zebrafish Embryos to SnCl2 and OECD 212
(Early Life Stage Toxicity of SnCl2 in Zebrafish)
GLP compliance:
not specified
Remarks:
not specified in report
Analytical monitoring:
yes
Vehicle:
yes
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
120 h
Duration:
120 h
Dose descriptor:
NOEC
Effect conc.:
25 µmol/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
larval development
Remarks on result:
other: Embryo development
Duration:
120 h
Dose descriptor:
LOEC
Effect conc.:
50 µmol/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
larval development
Remarks on result:
other: Embryo development

The results of the current study show that the tested compound induced early life stage toxicity in zebrafish embryos and larvae when embryos were exposed from 6 to 120 h. It was revealed that exposure to low doses of SnCl2 (10, 25 lM) did not significantly affect the embryos’ survival or cause obvious malformation during the embryonic stages (6–120 hpf). However, exposure to higher concentrations of SnCl2 (50, 100, and 250 lM) reduced survival and caused changes in embryonic development, including tail deformation, reduced body growth, smaller head and eyes, bent trunk, mild pericardial edema, and smaller caudal fin. These data enriched our knowledge of the influence of SnCl2 on embryonic development. These results were in good agreement with those reported in previous studies related to zebrafish and other fish species.

µmol / L M mg/L M mg/L
SnCl_2 0 189,6 0,00 SnSO_4 214,77 0,00
10 189,6 1,90 214,77 2,15
NOEC Embryos (Malformation)  25 189,6 4,74     214,77 5,37
50 189,6 9,48 214,77 10,74
NOEC Adukt (food consumption) 100 189,6 18,96     214,77 21,48
250 189,6 47,40 214,77 53,69
Validity criteria fulfilled:
yes
Conclusions:
results clearly indicate a significant dose-dependent increase of developmental and genetic toxicity due to exposure to SnCl2. The NOEC was
determined to be 25 µM SnCl_2 (=5.37 mg SnSO_4 / L)

Description of key information

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Dose descriptor:
NOEC
Effect concentration:
5 mg/L

Additional information

NOEC for emryo development zebrafish