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EC number: 215-236-1 | CAS number: 1314-56-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2010-02-09 to 2010-07-02
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline Study with GLP, no deviations
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Diphosphorus pentaoxide
- EC Number:
- 215-236-1
- EC Name:
- Diphosphorus pentaoxide
- Cas Number:
- 1314-56-3
- Molecular formula:
- O10P4
- IUPAC Name:
- tricyclo[3.3.1.1³,⁷]tetraphosphoxane-1,3,5,7-tetrone
- Details on test material:
- - Name of test material (as cited in study report): Phosphorpentoxid
- Molecular formula: P4O10
- Molecular weight: 283.9 g/mol
- Physical state: Solid, white powder
- Analytical purity: 99.7 % (m/m)
- Purity test date: 2009-09-23
- Lot/batch No.: DEH2 018965
- Expiration date of the lot/batch: 2011-09-08
- Storage condition of test material: Stored in a desiccator at room temperature, protected from light.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: The concentration of the test item were analytically determined from all test concentrations and the control after 0 h (test start) and 72 h (test end).
- Sampling method: Separate samples at start (provided without alga) were prepared. Samples at the end of the test were taken directly from the test replicates.
- Sample storage conditions before analysis:All samples were stored at 6 ± 2 °C until start of analysis, if necessary.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution of 200 mg/L was freshly prepared with dilution water and agitated. The pH-value was adjusted to the pH of the dilution water ± 0.2 by adding appropriate volumes of 1 M NaOH.
- Eluate: Dilution water
- Differential loading: 12.5 - 25.0 - 50.0 - 100 - 200 mg/L
- Controls: Six replicates (without test item) were tested under the same test conditions as the test replicates.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Desmodesmus subspicatus Chodat
- Strain: SAG 86.81
- Source (laboratory, culture collection): SAG Pflanzenphysiologisches Institut der Universitaet Goettingen, Nikolausberger Weg 18, D-37073 Goettingen, Germany
- Age of inoculum (at test initiation): 4 days
- Method of cultivation: Fresh stocks were prepared from Z-Agar. Light intensity amounted 35 - 70 µE x m-2 x s-1 for 24 h per day.
ACCLIMATION
- Culturing media and conditions (same as test or not): Same as test
- Any deformed or abnormal cells observed: No
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- No
Test conditions
- Hardness:
- Not measured
- Test temperature:
- Mean: 22.5 °C, Min: 22 °C, Max: 23 °C
- pH:
- Nominal test item concentration pH-value
[mg/L] Start; 0 h End; 72 h
200 8.06 7.26
100 8.03 7.32
50.0 7.98 7.29
25.0 7.97 7.36
12.5 7.94 7.45
Control 7.92 7.56 - Dissolved oxygen:
- Not measured
- Salinity:
- Not measured, freshwater
- Nominal and measured concentrations:
- Please refer to "Any other information on materials and methods incl. tables"
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL Erlenmeyer flasks
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: sterile 250 mL Erlenmeyer flasks, test volume 100 mL
- Aeration: Test containers were placed on a rotary shaker and oscillated at appr. 70 rpm
- Type of flow-through (e.g. peristaltic or proportional diluter): None
- Renewal rate of test solution (frequency/flow rate): One application at test start
- Initial cells density: 5074 cells/mL
- Control end cells density: Mean 1437745 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates): None
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dilution water, composition acc. to the guideline
- Hardness: 0.24 mmol Ca+Mg/L
- pH-value: 8.1 +/- 0.2
- Culture medium: Nutrient Medium Z acc. to LÜTTGE et al. (1994)
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: no
- Photoperiod: 24 h
- Light intensity and quality: 60 - 120 µE x m-2 x s-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: [counting chamber; electronic particle counter; fluorimeter; spectrophotometer; colorimeter] Fluorimeter, via Chlorophyll-a fluorescence (excitation at 436 nm, emission at 685 nm), cell concentrations were related to all cell density values acc. to a calibration curve.
TEST CONCENTRATIONS
- Range finding study: Yes
Results of the Range Finding Tests (0-72 h)
Results of the Range Finding Test (0-72 h)
Nominal
Test Item Concentration
[mg/L] Specific Growth Rate Inhibition [%] Yield Inhibition [%]
100 100 100
10 - 10 - 59
1 - 11 - 69
0.1 - 11 - 70
- Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrations
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 66.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: (64.0-69.7)
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test):
- Unusual cell shape: No
- Colour differences: No
- Flocculation: No
- Adherence to test vessels: No
- Aggregation of algal cells: No
- Other: No
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No
- Effect concentrations exceeding solubility of substance in test medium: Yes - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EC50:
Rate-related inhibition: 0.614 (CI 0.551 - 0.679) mg/L
Yield Inhibition: 0.333 (CI 0.318 - 0.350) mg/L - Reported statistics and error estimates:
- The NOEC and LOEC were determined by calculation of
statistical analyses statistical significance of growth rates and yield.
One Way Analysis of Variance was carried out for the determination of statistically significant differences compared to control replicates. When running a One Way Analysis of Variance a Normality Test and an Equal Variance Test were done first. P-values for both Normality and Equal Variance Tests were 0.05. The a-value for ANOVA was a=0.05. Normality test failed for yield calculation. Transformation (square root) was carried out for yield values.
Detection of statistically significant differences were considered negligible when inhibition values were ≤ 10 %. These findings were marked “biologically not significant”. Following the recommendations of the UBA (HEGER ET AL. 1998), inhibitions > 10 % are significant.
EC10-, EC20- and EC50-values of the growth rate and yield inhibition after 72 h were calculated by sigmoidal dose-response regression (EyCx). Calculation of the confidence intervals EC10-, EC20- and EC50-values were carried from the best fit values, the standard error and the t-distribution with the software Graph Pad Prism.
Any other information on results incl. tables
NOEC, LOEC, EC - values and 95 % Confidence Intervals of Phosphorpentoxid(0-72 h)
based on nominal test item concentrations [mg/L]
Rate-related Inhibition |
|
NOEC |
25.0 |
LOEC |
50.0 |
ErC10 |
39.4 (37.2 – 41.5) |
ErC20 |
48.6 (47.3 – 50.0) |
ErC50 |
66.5 (64.0 – 69.7) |
Inhibition of Yield |
|
NOEC |
12.5 |
LOEC |
12.5 |
EyC10 |
12.5 |
EyC20 |
16.3 ( 12.5 – 22.8) |
EyC50 |
38.0 (34.2 – 41.2) |
Cell Densities
|
Nominal test item concentration |
Replicate |
Cell density [cells/mL] |
||||||
[mg/L] |
No. |
0 h |
24 h |
48 h |
72 h |
|
|||
200 |
1 |
5074 |
17202 |
14943 |
15041 |
|
|||
2 |
5074 |
16952 |
17694 |
17226 |
|
||||
3 |
5074 |
13524 |
15443 |
14090 |
|
||||
Mean |
5074 |
15893 |
16027 |
15452 |
|
||||
100 |
1 |
5074 |
16046 |
16780 |
18432 |
|
|||
2 |
5074 |
17800 |
18707 |
20531 |
|
||||
3 |
5074 |
16517 |
19904 |
21130 |
|
||||
Mean |
5074 |
16788 |
18464 |
20031 |
|
||||
50.0 |
1 |
5074 |
19740 |
94868 |
429018 |
|
|||
2 |
5074 |
22499 |
93081 |
376378 |
|
||||
3 |
5074 |
21249 |
103429 |
440412 |
|
||||
Mean |
5074 |
21163 |
97126 |
415269 |
|
||||
25.0 |
1 |
5074 |
29559 |
197098 |
1055392 |
|
|||
2 |
5074 |
28600 |
203498 |
1018434 |
|
||||
3 |
5074 |
30199 |
213026 |
1058897 |
|
||||
Mean |
5074 |
29453 |
204541 |
1044241 |
|
||||
12.5 |
1 |
5074 |
34635 |
218225 |
1151348 |
|
|||
2 |
5074 |
28288 |
171641 |
1047757 |
|
||||
3 |
5074 |
31822 |
271312 |
1317160 |
|
||||
Mean |
5074 |
31582 |
220393 |
1172088 |
|
||||
Control |
1 |
5074 |
37152 |
269122 |
1413473 |
|
|||
2 |
5074 |
31576 |
237019 |
1387574 |
|
||||
3 |
5074 |
28223 |
234740 |
1193152 |
|
||||
4 |
5074 |
35246 |
302422 |
1489901 |
|
||||
5 |
5074 |
35365 |
303890 |
1542668 |
|
||||
6 |
5074 |
40695 |
332717 |
1599703 |
|
||||
Mean |
5074 |
34710 |
279985 |
1437745 |
|
Evaluation after 72 h
Statistically significant differences of growth rates and yield compared to
control values are marked (+), not significant differences are marked (-).
Nominal test item concentration |
Replicate |
Growth rate |
Rate-related inhibition |
Yield |
Inhibition of yield |
|
|||
[mg/L] |
No. |
[d-1] |
[%] |
[cells/mL] |
[%] |
|
|||
200 |
1 |
0.362 |
80.7 |
9967 |
99.3 |
||||
2 |
0.407 |
78.3 |
12152 |
99.2 |
|||||
3 |
0.340 |
81.9 |
9016 |
99.4 |
|||||
Mean |
(+) |
0.370 |
80.3 |
(+) |
10378 |
99.3 |
|||
100 |
1 |
0.430 |
77.1 |
13358 |
99.1 |
||||
2 |
0.466 |
75.2 |
15457 |
98.9 |
|||||
3 |
0.476 |
74.7 |
16056 |
98.9 |
|||||
Mean |
(+) |
0.457 |
75.7 |
(+) |
14957 |
99.0 |
|||
50.0 |
1 |
1.48 |
21.4 |
423944 |
70.4 |
||||
2 |
1.44 |
23.7 |
371304 |
74.1 |
|||||
3 |
1.49 |
20.9 |
435338 |
69.6 |
|||||
Mean |
(+) |
1.47 |
22.0 |
(+) |
410195 |
71.4 |
|||
25.0 |
1 |
1.78 |
5.40 |
1050318 |
26.7 |
||||
2 |
1.77 |
6.03 |
1013360 |
29.3 |
|||||
3 |
1.78 |
5.34 |
1053823 |
26.4 |
|||||
Mean |
(+)* |
1.78 |
5.59 |
(+) |
1039167 |
27.5 |
|||
12.5 |
1 |
1.81 |
3.86 |
1146274 |
20.0 |
||||
2 |
1.78 |
5.53 |
1042683 |
27.2 |
|||||
3 |
1.85 |
1.48 |
1312086 |
8.42 |
|||||
Mean |
(+)* |
1.81 |
3.62 |
(+) |
1167014 |
18.5 |
|||
Control |
1 |
1.88 |
1408399 |
||||||
2 |
1.87 |
1382500 |
|||||||
3 |
1.82 |
1188078 |
|||||||
4 |
1.89 |
1484827 |
|||||||
5 |
1.91 |
1537594 |
|||||||
6 |
1.92 |
1594629 |
|||||||
Mean |
1.88 |
1432671 |
Section-by-Section and Average
Specific Growth Rates of the Control Group
(0
– 72 h)
Replicate No. |
Specific growth rate [d-1] |
Mean (0 - 72 h) |
SD ± |
CV |
Mean CV [%] |
|||
section-by-section |
||||||||
0 - 24 h |
24 - 48 h |
48 - 72 h |
||||||
Control |
1 |
1.99 |
1.98 |
1.66 |
1.88 |
0.189 |
10.1 |
12.6 |
2 |
1.83 |
2.02 |
1.77 |
1.87 |
0.130 |
6.93 |
||
3 |
1.72 |
2.12 |
1.63 |
1.82 |
0.262 |
14.4 |
||
4 |
1.94 |
2.15 |
1.60 |
1.89 |
0.280 |
14.8 |
||
5 |
1.94 |
2.15 |
1.63 |
1.91 |
0.265 |
13.9 |
||
6 |
2.08 |
2.10 |
1.57 |
1.92 |
0.301 |
15.7 |
||
Mean |
1.88 |
|
||||||
± SD |
0.03 |
|
||||||
CV [%] |
1.84 |
|
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this study Phosphorpentoxid was found to inhibit the growth of the freshwater green alga Desmodesmus subspicatus after 72 h with the following effect values (nominal test item concentrations): The EC50-values with 95 % confidence intervals for inhibition of specific growth rate (ErC50) and yield (EyC50) after 72 h were 66.5 (64.0 – 69.7) and 38.0 (34.2 – 41.2) mg/L, respectively.
All effect levels are given based on nominal concentrations of Phosphorpentoxid. - Executive summary:
The toxicity of Phosphorpentoxid(batch number DEH2 018965) to the unicellular freshwater green alga Desmodesmus subspicatus was determined according to the principles of OECD 201 at Dr.U.Noack-Laboratorienin 31157 Sarstedt, Germany from February 9th to July 2nd with the definitive exposure phase from June 29th to July 2nd, 2010. The aim of the study was to assess the effects on growth rate and yield over a period of 72 h.
The study was conducted under static conditions with an initial cell density of approximately 2 – 5 x 103cells/mL. Five concentration levels were tested in a geometrical series with a dilution factor of 2, nominal: 12.5 - 25.0 - 50.0 - 100 - 200 mg/L. In order to exclude possible effects due to the pH-value all test concentrations were tested by correction of the pH-value. Three replicates were tested for each test item concentration and six replicates for the control. Environmental conditions were determined to be within the acceptable limits.
The concentrations of Phosphorpentoxidwere analysed at all concentration levels after 0 and 72 h via standardized photometric test. The measured concentrations of Phosphorpentoxidat test start were in the range of 89 – 93 % of the nominal values. At the end of the test Phosphorpentoxidgave recoveries of 79 – 97 % of the nominal values. All effect values are given based on nominal concentrations of the test item.
NOEC, LOEC, EC - values and 95 % Confidence Intervals of Phosphorpentoxid(0-72 h)
based on nominal test item concentrations [mg/L]
Rate-related Inhibition
NOEC
25.0
LOEC
50.0
ErC10
39.4 (37.2 – 41.5)
ErC20
48.6 (47.3 – 50.0)
ErC50
66.5 (64.0 – 69.7)
Inhibition of Yield
NOEC
12.5
LOEC
12.5
EyC10
12.5
EyC20
16.3 ( 12.5 – 22.8)
EyC50
38.0 (34.2 – 41.2)
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