Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 240-282-4 | CAS number: 16111-62-9
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
The 48-hour EC50 of bis(2-ethylhexyl) peroxydicarbonate to Daphnia magna was 9.4 mg/L (EU C.2, OECD 202).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 9.4 mg/L
Additional information
The 48-hr acute toxicity of bis(2-ethylhexyl) peroxydicarbonate (75.3 % in isododecane) to Daphnia magna was studied under static conditions according to method EU C.2 and OECD 202. Daphnids were exposed to nominal concentrations of 0, 9.9, 14.8, 22.2, 33.3, and 50 mg/L for 48 hours. The test item is a mixture of 2 constituents with different solubilities and physicochemical properties. Test solutions were prepared according to the WAF methodology (water accommodated fraction). Mortality/immobilisation was observed daily.
Concentrations of the test item were measured by gas chromatography coupled with mass spectrometry. The parent substance was quantified via its degradation product 2-ethyl hexanol. The final concentrations of bis(2-ethylhexyl) peroxydicarbonate were not all maintained within the designated limit of 80 % of initial. Thus effective concentrations were calculated using geometric means of final and initial measured concentrations of the test item.The 48-hour EC50was 9.4 mg/L.
This study is considered as acceptable and satisfies the guideline requirements for an acute toxicity study with freshwater invertebrates.
Results synopsis
EC50 (24 h) and EC50 (48 h) values are summarized in the following table:
Effective concentration (mg/l) |
Value |
95 % CI |
EC50 (24 h) |
32 |
ND |
EC50 (48 h) |
9.4 |
ND |
CI=confidence interval; ND=not determined
Please note: The test was done according to the WAF procedure (since the test item contains 25 % isododecane). Measurements were made for the degradation product 2-ethylhexanol by GC-MS. Isododecane is stated to be practically insoluble in water (no exact values available). The water solubility of the degradation product 2-ethyl hexanol is much higher compared to isododecane. However, isododecane may contribute to the overall toxicity observed in the test. Therefore, the test can be regarded as a worst-case that can be used in the assessment of the pure substance (which is, however, not stable without the additive).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
