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EC number: 203-047-7 | CAS number: 102-69-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline study (OECD 471), with a deviation (2-AA was used as sole indicator of the efficiancy of the S9-mix
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- 2-AA was used as sole indicator of the efficacy of the S9-mix.
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Tripropylamine
- EC Number:
- 203-047-7
- EC Name:
- Tripropylamine
- Cas Number:
- 102-69-2
- Molecular formula:
- C9H21N
- IUPAC Name:
- tripropylamine
- Test material form:
- other: solution
- Details on test material:
- - Name of test material (as cited in study report): Tripropylamin
- Physical state: colorless liquid
- Analytical purity: 98.7 g/100 g (GC)
- Lot/batch No.: Behälter 960
- Storage condition of test material: room temperature
Constituent 1
Method
- Target gene:
- his operon, trp operon
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
- Metabolic activation:
- with and without
- Metabolic activation system:
- The S9 fractions were prepared from the liver of Aroclor 1254-induced male Sprague-Dawley rats
- Test concentrations with justification for top dose:
- Standard plate test: 0, 20, 100, 500, 2500, 5000 µg/plate (S. typhimurium and E. coli)
Preincubation test: 0, 4, 20, 100, 500, 2500 µg/plate (S. typhimurium); 0, 20, 100, 500, 2500, 5000 µg/plate (E. coli). - Vehicle / solvent:
- - Vehicle/solvent used: acetone
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- acetone
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: with S-9 mix: all strains 2-aminoanthracene; without S-9 mix: strains TA100, TA1535: N-methyl-N'-nitro-N-nitrosoguanidine; TA98: 4-nitro-o-phenylendiamine; TA1537: 9-aminoacridine; E.coli WP2 uvrA: N-ethyl-N'-nitro-N-nitrosoguanidine.
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation
DURATION
- Preincubation period: 20 min
- Exposure duration: 48 h
NUMBER OF REPLICATIONS: 3
DETERMINATION OF CYTOTOXICITY
- Method: relative total growth
METHOD OF APPLICATION: standard plate test
DURATION
- Exposure duration: 48 h
NUMBER OF REPLICATIONS: 3
DETERMINATION OF CYTOTOXICITY
- Method: relative total growth - Evaluation criteria:
- The test chemical is considered positive in this assay if the following criteria are met: A dose-related and reproducible increase in the number of revertant colonies, i.e. about doubling of the spontaneous mutation rate in at least one tester strain either without S-9 mix or after adding a metabolizing system.
A test substance is generally considered non-mutagenic in this test if: The number of revertants for all tester strains are within the historical negative control range under all experimental conditions in two experiments carried out independently of each other.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Standard plate test: depending on the strain and test conditions from about 2,500 µg/plate onward. Preincubation assay: depending on the strain and test conditions from about 100 µg - 500 µg/plate onward.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- In the preincubation assay bacteriotoxicity was observed depending on the strain and test conditions from about 2500 µg/plate onward.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation: No test substance precipitation was found. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Standart plate test:
Dose (µg/plate) | TA1535 | TA100 | TA1537 | TA98 | E. coli WP2 uvrA | |||||
-S9 | +S9 | -S9 | +S9 | -S9 | +S9 | -S9 | +S9 | -S9 | +S9 | |
0 | 19±1 |
19±1 |
105±4 |
112±3 |
9±1 |
10±1 |
24±2 |
33±3 |
32±4 |
31±4 |
20 |
18±2 |
15±1 |
103±6 |
108±8 |
10±1 |
11±2 |
21±3 |
30±1 |
29±4 |
30±3 |
100 |
14±3 |
16±2 |
98±7 |
108±2 |
9±1 |
11±2 |
18±1 |
34±3 |
27±1 |
32±5 |
500 |
15±2 |
16±3 |
103±3 |
117±6 |
6±2 |
10±2 |
19±1 |
27±6 |
24±3 |
34±1 |
2500 |
6±3 |
13±2 |
100±2 |
106±8 |
7±2 |
7±1 |
20±1 |
25±3 |
24±2 |
28±3 |
5000 |
10± |
14±4X |
102±3 |
94±6X |
7±2 |
4±2X |
16±2 |
29±4 |
25±5 |
28±7 |
2 -AA |
- |
156±1 |
- |
1191±131 |
- | 187±4 |
- |
1823±73 |
- |
199±7 |
MNNG |
848±44 |
- |
1005±8 |
- |
- |
- |
- |
- |
- |
- |
AAC |
- |
- |
- |
- |
1204±7 |
- |
- |
- |
- |
- |
NOPD |
- |
- |
- |
- |
- |
- |
698±17 |
- |
- |
- |
ENNG |
- |
- |
- |
- |
- |
- |
- |
- |
940±50 |
- |
Mean ± SD
Preincubation-test:
Dose (µg/plate) |
TA1535 |
TA100 |
TA1537 |
TA98 |
E. coli WP2 uvrA |
|||||
|
-S9 |
+S9 |
-S9 |
+S9 | -S9 | +S9 | -S9 | +S9 | -S9 | +S9 |
0 | 21±1 |
20±2 |
115±3 |
111±9 |
11±2 |
11±1 |
29±2 |
39±1 |
28±3 |
37±6 |
4 |
19±0 |
17±1 |
110±15 |
106±10 |
9±2 |
10±2 |
26±3 |
26±5 |
- |
- |
20 |
18±3 |
17±3 |
103±8 |
112±9 |
9±2 |
9±1 |
20±2 |
25±6 |
26±4 |
27±5 |
100 |
17±1 |
16±3 |
103±8 |
108±7 |
9±2 |
8±2 |
18±2 |
23±2 |
23±2 |
22±2 |
500 |
15±2 |
11±2 |
113±10 |
105±14 |
10±1 |
6±2 |
17±4 |
21±2 |
21±2 |
19±3 |
2500 |
X |
X |
X |
X |
X |
X |
X |
X |
18±4 |
16±4 |
5000 |
- |
- |
- |
- |
- |
.- |
- |
- |
X |
X |
2-AA |
- |
140±33 |
- |
1039±31 |
- |
150±8 |
- |
973±20 |
- |
210±12 |
MNNG |
1166±47 |
- |
1329±21 |
- |
- |
- |
- |
- |
- |
- |
AAC |
- |
- |
- |
- |
697±27 |
- |
- |
- |
- |
- |
NOPD |
- |
- |
- |
- |
- |
- |
677±4 |
- |
- |
- |
ENNG | - | - | - | - |
- | - | - | 903±66 | - |
Mean ± SD
X: reduced background growth
2-AA: 2-aminoanthracene;
MNNG; N-methyl-N-nitro-N-nitrosoguanidine
ENNG; N-ethyl-N-nitro-N-nitrosoguanidine
NOPD: 4-nitro-o-phenylendiamine
AAC: 9-aminoacridine chloride monohydrate
Under the conditions tested Tripropylamine is non mutagenic in the bacterial AMES-test.
The positive controls gave the expected values.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Tripropylamine was negative in the Standard Plate Test and in the Preincubation Test. - Executive summary:
Tri-n-propylamine was tested for its mutagenic activity in the Salmonella - Escherichia coli/Mammalian-Microsome Reverse Mutation Assay. The tester strains used in the mutagenicity assay were Salmonella typhimurium tester strains TA98, TA100, TA1535, TA1537, and Escherichia coli tester strain WP2uvrA. The assay was conducted with five doses of the test article in the presence and the absence of S9 mix along with concurrent vehicle and positive controls using three plates per dose. The doses tested were 0, 20, 100, 500, 2500, 5000 µg/plate in the standard plate test (Salmonella typhimurium and E. coli) and 0, 4, 20, 100, 500, 2500 µg/plate (Salmonella typhimurium) and 0, 20, 100, 500, 2500, 5000 µg/plate (E. coli) in the preincubation test. The experiments were performed in the presence and absence of S9 mix.
The results show that, under the conditions of this study, tri-n-propylamine did not cause a positive increase in the number of revertants per plate of any of the tester strains either in the presence or absence of microsomal enzymes prepared from Aroclor™-induced rat liver (S9).
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