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EC number: 231-626-4 | CAS number: 7659-86-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- November 26, 2008 to February 05, 2009
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Principles of method if other than guideline:
- The test item 2-Ethylhexyl Thioglycolate is a volatile substance and therefore the test conditions according to the OECD 209 test guideline were not applicable. A modified test design was used to fulfil first the requirements of the OECD 209 and second the volatile character of the test item. The
activated sludge microorganisms were exposed to the test item for three hours in a closed system with a headspace of air and intensively mixing.
This test method of application and the test system are recommended by the test guidelines for volatile test items. - GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-ethylhexyl mercaptoacetate
- EC Number:
- 231-626-4
- EC Name:
- 2-ethylhexyl mercaptoacetate
- Cas Number:
- 7659-86-1
- Molecular formula:
- C10H20O2S
- IUPAC Name:
- 2-ethylhexyl 2-sulfanylacetate
- Details on test material:
- - Name of test material (as cited in study report): 2-Ethylhexyl Thioglycolate
- Physical state: Liquid at RT, Colorless
- Analytical purity: 99.90%
- Density: 0.974 g/ml (20¿)
- Vapor Pressure: 0.01 hPA(20 ¿)
- Lot/batch No.: 26.05.08.D
- Expiration date of the lot/batch: May 2010
- Storage condition of test material: In original container, at room temperature (10 - 30 ¿), in the dark
Constituent 1
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Test System Species/Origin: Activated sludge, micro organisms from a domestic waste
water treatment plant was supplied by the municipal sewage treatment plant Darmstadt, Germany.
Conditioning: The activated sludge was washed by centrifugation and the supernatant liquid phase was decanted, The solid material was resuspended in tap water and again centrifuged, The latter procedure was repeated three times, The washed sludge was resuspended in tap water and sewage feed (50 mL per L, see was added, The sludge was kept at room temperature under continuous aeration until use for two days. To this mix-ture, 50 mL synthetic sewage feed per litre was added daily, starting three days prior to use, and the sludge was kept at room temperature under continuous aeration until use, Immediately before use, the dry weight of the activated sludge was determined, and diluted to 3,8 giL with tap water. The pH of the activated sludge was 7,6 and therefore no adjustment was necessary.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 180 min
Test conditions
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 180 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 180 min
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- Inhibition of Respiration Rate: The influence of 2-Ethylhexyl Thioglycolate on the respiration rate of activated sludge is presented in Table 2. In comparison to the inoculum controls the respiration rate of the activated sludge was slightly inhibited by 5,1 % and 7,7% % at the nominal concentrations from 1 to 32 mg/L, respectively. At the highest nominal concentration of 100 mg/L, the respiration rate was moderately inhibited by 15.4%, respectively, Test item concentrations exceeding 100 mg/L were not tested.
3-Hours EC20 and 3-hours EC50: Based on measured inhibition rates, no 3-hour EC20 and EC50 could be quantified because up to the highest nominal test concentration of 100 mg/L less than 20 % inhibition was noted after three hours incubation, Nevertheless, the 3-hour EC20 and EC50 are clearly higher than 100 mg/L under the present test conditions. - Results with reference substance (positive control):
- Inhibition of Respiration Rate: The inhibition of the activated sludge treated with the reference item was in the range of 20% to 80 % for the test concentrations of 3,2, 10 and 32 mg/L.
3-Hours EC50 of 3,5-Dichlorophenol: The 3-hour EC10, EC20 and EC50 and their 95 % confidence limits of 3,5-Dichlorophenol were calculated. The results are listed below in Table 3.
Any other information on results incl. tables
Table 2. Influence of 2~Ethylhexyl Thioglycolate and the Reference Item 3,5-Dichlorophenol on |
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Oxygen Consumption of Activated Sludge
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Flask |
Treatment |
Concen- |
Oxygen |
Inhibition |
pH-values |
Oxygen |
||
No. |
|
tration |
consumption |
|
|
|
concentration |
|
|
|
|
|
|
|
|
[mg O2/L] |
|
|
|
[mg/L] |
[mg O2/L min] |
% |
start* |
end* |
start* |
end* |
1 |
control |
--- |
0.42 |
--- |
7.4 |
8.0 |
7.7 |
8.2 |
10 |
control |
--- |
0.36 |
--- |
7.4 |
7,9 |
7.6 |
8.0 |
|
|
|
-- |
|
|
|
|
|
|
|
mean |
0.39 |
|
|
|
|
|
|
% replicate deviation |
7,7 |
|
|
|
|
|
|
2 |
3,5-DCP |
32 |
0.09 |
76,9 |
7.4 |
7,9 |
7.6 |
8.3 |
3 |
3,5-DCP |
10 |
0.20 |
48,7 |
7.4 |
7.9 |
7.5 |
8.0 |
4 |
3,5-DCP |
3.2 |
0.31 |
20,5 |
7.4 |
7.9 |
7.5 |
8.1 |
|
- |
|
|
|
|
|
|
|
5 |
Test item |
100 |
0.33 |
15.4 |
n. d. |
8.0 |
n. d, |
8.0 |
6 |
Test item |
32 |
0.37 |
5.1 |
n. d. |
7,9 |
n. d, |
8.0 |
7 |
Test item |
10 |
0.37 |
5.1 |
n. d. |
7.9 |
n. d. |
8.1 |
8 |
Test item |
3.2 |
0,37 |
5,1 |
n. d. |
7,9 |
n. d. |
8.1 |
9 |
Test item |
1 |
0,36 |
7,7 |
n. d. |
7,9 |
n. d, |
8.0 |
*: Start and end of 3-hour aeration |
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n. d.: not determined due to the volatility of the test item |
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DCP: 3,5-Dichlorophenol |
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Test item: 2-Ethylhexyl Thioglycolate |
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Table 3: Summary of EC10, EC20, and EC50-values with 3,5-Dichlorophenol.
Parameter |
EC10 |
EC20 |
EC50 |
Value [mgll] |
1.6 |
3.1 |
10,6 |
lower 95%-cl |
0,9 |
2.1 |
8.8 |
upper 95%-cl |
2.3 |
4.1 |
12,8 |
|
The following nominal concentrations of the positive control 3,5 Dichlorophenol were tested on the same activated sludge and under identical conditions as the test item: 32, 10 and 3.2 mg/L. In comparison to the controls the respiration rate of the activated sludge was moderately inhibited by 20.5% at the lowest nominal concentration of 3.2 mg/L. At the nominal concentrations of 10 and 32 mg test item/L, the respiration ate was inhibited by 48.7% and 76,9%, respectively.
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Applicant's summary and conclusion
- Executive summary:
-
The influence of the test item 2-Ethylhexyl Thioglycolate on the activity of activated sludge was evaluated by measuring the respiration rate under defined conditions. The respiration rate (oxygen consumption) of an aerobic activated sludge fed with a standard amount of synthetic sewage was measured in the presence of various concentrations of the test item after an incubation period of 3 hours, according to the OECD Guideline 209.
In comparison to the inoculum controls the respiration rate of the activated sludge in presence of the thest item 2-Ethylhexyl Thioglycolate was slightly inhibited by 5,1 % and 7,7% % at the nominal concentrations from 1 to 32 mg/L, respectively. At the highest nominal concentration of 100 mg/L, the respiration rate was moderately inhibited by 15.4%, respectively, Test item concentrations exceeding 100 mg/L were not tested.
Based on measured inhibition rates, no 3-hour EC20 and EC50 could be quantified because up to the highest nominal testitem concentration of 100 mg/L less than 20 % inhibition was noted after three hours incubation. Nevertheless, the 3-hour EC20 and EC50 are clearly higher than 100 mg/L under the present test conditions.
The inhibition of the respiration rate in the presence the positive test control item 3,5-Dichlorophenol was in the range of 20% to 80 % for the test concentrations of 3,2, 10 and 32 mg/L. The 3-hour EC10, EC20 and EC50 and their 95 % confidence limits of 3,5-Dichlorophenol were calculated and found to 1.6, 3.1, and 10.6 mg/L, repectively.
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