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Diss Factsheets
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EC number: 439-590-3 | CAS number: 12158-75-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- May 1 -June 7, 1975
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non GLP Study (dated 1977)
Data source
Referenceopen allclose all
- Reference Type:
- secondary source
- Title:
- Unnamed
- Year:
- 1 977
- Reference Type:
- publication
- Title:
- Toxicity of Metal Mining Wastes
- Author:
- Hale J.G.
- Year:
- 1 977
- Bibliographic source:
- Bulletin of Environmental Contamination and Toxicolgy, Vol 17, N°1
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: U.S. EPA Standard Methods No. 810 B.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Nitric Acid, Copper (2+) salt
- IUPAC Name:
- Nitric Acid, Copper (2+) salt
- Details on test material:
- CAS# 3251-23-8
Constituent 1
Sampling and analysis
- Details on sampling:
- Test water fo bioassay was prepared by addinig a stock concentration of reagent grade chemical to Whitewood Creek water. The chemical used wascupric nitrate Cu(NO3)2. Stock solution was made-up on the basis of active ingredient (Copper).
Test organisms
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- Young-of-the-year (2-month-old) rainbow trout (Salmo gairdineri Richardson). The total length of these fish ranged from 51 to 76 mm.
Study design
- Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- pH:
- pH ranged from 6.4 to 8.3
- Dissolved oxygen:
- Dissolved oxygen ranged from 4.8 to 9.0 mg/L
- Salinity:
- Total alkalinity ranged from 82 to 132 mg/L as CaCO3.
- Nominal and measured concentrations:
- Cu < 30 µg/L
- Details on test conditions:
- Test chambers were made of glass and had a 8-liter capacity. Bioassay exposures were in duplicate at each test concentration with ten fish in each chamber. The minimum volumetric turnover for any chamber was 6.5 times in 24 hours.
Test water was monitored daily for pH, temperature, dissolved oxygen, total alkalinity and heavy metals. One test chamber was continuously monitored for temperature with a recording thermocouple.
Fish mortalities were recorded at 24-hours intervals. LC50 values were estimated using a straight-line graphic interpolation (Standard methods, 1971). - Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 253 µg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- element
- Basis for effect:
- mortality (fish)
- Details on results:
- Under the conditions of the test, the 96-hour LC50 value for Copper, using a 2-month-old rainbow trout, was 253 µg Cu/L. Range tested was 0.078 to 1.250 mg Cu/L.
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Free ionic copper is considered the active ingredient in inorganic copper salts, and is believed to be responsible for adverse effects. The bioavailablity of the copper ion at target sites determines the severity of effects. Copper nitrate is more water soluble than Copper hydroxide nitrate and provides a conservative estimate of acute aquatic toxicity.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the test, the 96-hour LC50 value for Copper, using a 2-month-old rainbow trout, was 253 µg Cu/L. Based on a content of 53% Copper in Basic Copper Nitrate, the 96-hour LC50 value for this substance is estimated to be 477 µg/L.
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