Methyl tetrahydro-2-furancarboxylate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From april 22 to april 26, 1996

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP, guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Methyl tetrahydro-2-furancarboxylate

EC Number:

420-670-1

EC Name:

Methyl tetrahydro-2-furancarboxylate

Cas Number:

37443-42-8

Molecular formula:

C6H10O3

IUPAC Name:

methyl oxolane-3-carboxylate

Details on test material:

Identity: Methfat
Chemical name: Methyl tetrahydro-2-furoate
Batch number: 17-5C03 (GLFC No.002)
Expiry: 7 November 1996
Purity: 99.4%
Appearance: Clear liquid
Storage conditions: In darkness at room temperature
Date received: 17 November 1995

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Oncorhynchus mykiss
- Source: Westacre Trout Farm, Norfolk, UK.
- Length at study end (mean): 5.1 cm (SD = 0.36 cm)
- Weight at study (mean): 1.81 g (SD = 0.37g)
- Feeding during test: fish were fed daily to repletion but food was not given during the 24 hourperiod immediately prior to exposure or during the exposure period itself.
- Food type: commercial fry pellet

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Test conditions

Hardness:

calculated from daily measurements during the exposure period = 141 mgCaCO3/l (SD = 8.5 mg)

Test temperature:

15 +/- 2°C

Dissolved oxygen:

9.6 +/- 5 mgO2/l

Nominal and measured concentrations:

Nominal concentrations: 4.6, 10, 22, 46 and 100 mg/l
Mean measured concentrations: 4.5, 10, 22, 46 and 99 mg/l

4.6, 10, 22, 46 and 100 mg/1
4.5, 10, 22, 46 and 99 mg/1

Details on test conditions:

TEST SYSTEM
- Material, size, headspace, fill volume: Animals were placed at random in glass aquaria containing prepared test media or test water only. Each vessel contained 40 litres of medium to a depth of 26 cm (approximate dimensions of vessel: 52.5 x 32 x 38 cm).
- Aeration: Supplementary aeration was not provided in this study, since methfat was initially considered to be volatile.
- Renewal rate of test solution (frequency/flow rate): Animals were exposed to the test or control conditions for a period of 96 hours with daily batchwise renewal of the teat media to ensure the maintenance of satisfactory environmental conditions and near nominal exposure levels.
- No. of organisms per vessel: Five test concentrations plus one control. Seven animals per test concentration.
- Biomass loading rate: 0.32 g bw/l (static volume)

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Laboratory tap water filtered, dechlorinated and softened by passage through an Elga® water purification system.
- Chlorine: remained below the limit of detection throughout the acclimatisation and exposure periods.

OTHER TEST CONDITIONS
- Photoperiod: 16 h light : 8 h dark
- Treatment and control groups were maintained at 14 ± 1°C throughout the exposure period.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

3 h

Dose descriptor:

LC50

Effect conc.:

> 99 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

6 h

Dose descriptor:

LC50

Effect conc.:

> 99 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

56 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 41-76 mg/l

Effect concentrations 4

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

40 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 28-57 mg/l

Effect concentrations 5

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

32 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 22-46 mg/l

Effect concentrations 6

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

32 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 22-46 mg/l

Effect concentrations 7

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

10 mg/L

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Measured concentrations ranged from 97- 110% of nominal in fresh media and from 93 - 104% of nominal in expired media, demonstrating that near nominal concentrations were achieved with each media renewal and maintained throughout each subsequent 24 hour exposure period of the study.

Highest test concentration resulting in 0% mortality: 22 mg/l
Lowest test concentration resulting in 100% mortality: 46 mg/l

Marked reactions to exposure (other than death) were increased pigmentation, swimming at surface, loss of equilibrium and slight lethargy.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 96 h LC50 value for Methfat with rainbow trout is 32 mg/l. The NOEC is 10 mg/l.