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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2007

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
1992
Deviations:
yes
Remarks:
temperature 21-24°C with a range of 3°C
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
3-bromo-5-chlorophenol
EC Number:
611-435-6
Cas Number:
56962-04-0
Molecular formula:
C6H4BrClO
IUPAC Name:
3-bromo-5-chlorophenol

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
samples were taken at start of the test and after 96 hours
samples with test substance concentration 2.1 mg/l were taken after 24 hours, 4.5 and 10 mg/l were taken after 2 hours, because all fish were dead

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Origin of test organisms: Institute of Zoology, Heidelberg (Germany), breed at the testing facility
juvenile fish
male and female
mean length: 2.45 cm

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
2.5 mmol/L
Test temperature:
21-24 °C with a range of 3 °C
pH:
6.5 - 8.5
Dissolved oxygen:
8.3 - 8.8
Nominal and measured concentrations:
10, 4.5, 2.1, 0.94 and 0.43 mg/l nominal
analytical measurements: 87 % of nominal concentration at test start, 86 % of nominal concentration after 96 hours

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
1.8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: 1.2 -2.9
Reported statistics and error estimates:
calculation of LC50 values and 95 % confidence limits by Probit analysis

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
GHS relevant (LC50 <10 mg/L)