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EC number: 202-046-9 | CAS number: 91-17-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1989-09-25 to 1989-10-31
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions: TA 102 or E.coli WP2 were not tested (not required by applied version of guideline).
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- Version / remarks:
- Cited as Directive 84/449/EEC, B.14
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Decahydronaphthalene
- EC Number:
- 202-046-9
- EC Name:
- Decahydronaphthalene
- Cas Number:
- 91-17-8
- Molecular formula:
- C10H18
- IUPAC Name:
- decahydronaphthalene
- Details on test material:
- Decahydronaphthalene of Hüls AG, purity 99 %, sample ID 734/881018
Constituent 1
Method
- Target gene:
- mutated gene loci responsible for histidine auxotrophy
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: strainsTA 1535, TA 1537, TA 1538, TA 98, TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254 induced rat S9 liver, male Bor: WISW (SPF/Cpb) rats
- Test concentrations with justification for top dose:
- 8; 40; 200; 1000; 5000 µg/plate
- Vehicle / solvent:
- solvent acetone (CAS No. 67-64-1)
Controls
- Untreated negative controls:
- yes
- Remarks:
- untreated
- Negative solvent / vehicle controls:
- yes
- Remarks:
- solvent controls
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- sodium azide
- other: Aminoacridine TA 1537
- Details on test system and experimental conditions:
- Ames test
SYSTEM OF TESTING
- Metabolic activation system: Aroclor 1254 induced rat S9 liver, male Bor: WISW (SPF/Cpb) rats
ADMINISTRATION:
- Dosing:
main test: 8/40/200/1000/5000 µg/plate (+/- metabolic activation)
pre-incubation test: 125/250/500/1000/2000 µg/plate (+/- metabolic activation)
- Number of replicates: 3
- Application:
solvent acetone (CAS No. 67-64-1) main test 100 g/l, pre-incubation test 80 g/l
- Positive and negative control groups and treatment:
positive, TA 98 and TA 1538: nitrofluorene
positive, TA 100 and TA 1535: sodium azide
positive, TA 1537: aminoacridine
negative: untreated + solvent controls
activity of metabolic system: aminoanthracene / TA 100
- Pre-incubation: 30 minutes at 30 °C
incubation 96 hours at 37 °C - Evaluation criteria:
- CRITERIA FOR EVALUATING RESULTS:
mutagenic effects (i.e ratio of revertant rates treated/control >= 2) at <= 5000 µg/plate with generally positive dose-response relationship in
any strain - Statistics:
- no data
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: strains TA 1535, TA 1537, TA 1538, TA 98, TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: > 5000 µg/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: other: Salmonella typhimurium TA 1535, TA 1537, TA 1538, TA 98, TA 100
Any other information on results incl. tables
GENOTOXIC EFFECTS:
- With metabolic activation: None
- Without metabolic activation: Slight effects in TA 98 and TA 1537 in the main test and slight effects in TA 1537 in the pre-incubation test were associated with very low revertant rates in solvent control and not dose-related.
Thus they were not considered to be signs of mutagenicity:
-------------------------------------------------------
Revertant rates in pre-incubation test without metabolic activation:
TA 1537, untreated: 8; solvent: 7; 125 µg: 7; 250 µg: 9; 500 µg: 7; 1000 µg: 15; 2000 µg: 15
-------------------------------------------------------
Revertant rates in main test without metabolic activation:
TA 98, untreated: 11; solvent: 4; 8 µg: 4; 40 µg: 10; 200 µg: 4; 1000 µg: 5; 5000 µg: 15, precipitation
TA 1537, untreated: 7; solvent: 4; 8 µg: 7; 40 µg: 4; 200 µg: 12; 1000 µg: 3; 5000 µg: 0, toxic and precipitation
-------------------------------------------------------
The positive controls were functional.
PRECIPITATION CONCENTRATION: 5000 µg/plate
CYTOTOXIC CONCENTRATION:
TA 98: None (+/- S9)
TA 100: None (+/- S9)
TA 1535: 5000 µg/plate (+/- S9)
TA 1537: 5000 µg/plate (- S9)
TA 1538: None (+/- S9)
Applicant's summary and conclusion
- Conclusions:
- Decahydronaphthalene was not genotoxic in both the presence or absence of metabolic activation in a microbial mutagenicity assay.
- Executive summary:
In a reverse gene mutation assay in bacteria, strains TA98, TA100, TA1535, TA1537 and TA1538 of Salmonella typhimurium were exposed to decahydronaphthalene using acetone as a vehicle at concentrations of 8 - 5000 µg/plate, with and without S-9 activation in a preincubation assay according to EEC guidance Dir. 84/449/EEC, B.14.
The test article was tested at six dose levels along with appropriate vehicle and positive controls on the tester strains mentioned above in the presence and absence of S9-mix. All dose levels, vehicle and positive controls were plated in triplicate.
Cytotoxicity was noted at 1000 -5000 µg/plate.
No positive mutagenic responses were observed with any of the strains used, in the presence as well as in the absence of microsomal enzymes.These results were confirmed in an independent assay.
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