Registration Dossier
Registration Dossier
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EC number: 209-578-0 | CAS number: 586-62-9
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
An acute aquatic toxicity study was performed on zebrafish (Danio rerio) in accordance with OECD Guideline No 203.
The 96h-LC50 of terpinolene monoconstituent for freshwater fish is 0.805 mg/L.
Based on the results of this study, terpinolene monoconstituent is classified:
- dangerous for the environment according to Directive 67/548/EEC "R50/53: very toxic to aquatic organisms, may cause long-term adverse effects in the aquatic environment"
- and hazardous to the aquatic environment under Regulation (EC) No 1272/2008 (CLP) - Category Acute 1 and Category Chronic 1.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 0.805 mg/L
Additional information
Key study conducted according to OECD 203 Guideline and GLP without deviation.
Fish (Danio rerio) were exposed to terpinolene monoconstituent at nominal test concentrations of 0.6, 0.8, 1.1, 1.5 and 2.0 mg/L over a test period of 96 hours (semi-static test, daily renewal). Concentrations were measured at the start of the study and daily until the end of the test. The concentrations of the test item were not satisfactorily maintained within ± 20% of the initial concentrations throughout the test. Thus, the LC50 were based on the geometric mean of the measured concentrations. The 96h-LC50 determined in this study can be used as key value for chemical safety assessment.
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