Ethyl 2-methyl-1,3-dioxolane-2-acetate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

24 August 1999 - 26 August 1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Vehicle:

no

Details on test solutions:

To produce the only test concentration, the test substance was weighed into water, treated for 60 seconds at 8000 rpm with an ultra-turrax and afterwards stirred for 24 hours on a magnetic stirrer. To remove undissolved particles, the resulting suspension was filtered using a folded filter of pore size 7-12 µm.

Test organisms (species):

Daphnia magna

Details on test organisms:

Test organism
- Species: Daphnia magna STRAUS, parthenogenetic females
-Origin of species: cloned at the Federal Health Office (BGA) in Berlin
- Test animals: laboratory bred at Bayer AG Leverkusen
- Age of test animals: 0 - 24 hours
- Keeping water: M4-Medium according to Elendt and BGA (1992)
-Separation of test animals according to size before start of test: 1st sieving on 23/08/1999 at 2.00 p.m.; 2nd sieving on 24-08-1999 at 8.00 a.m.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

Hardness of M4-Medium 14.7 °dH

Test temperature:

20.3 °C

pH:

8.1

Dissolved oxygen:

8.4 - 8.6 mgO2/L

Nominal and measured concentrations:

Nominal concentration of 100 mg/L

Details on test conditions:

STUDY CONDITIONS
-Operating conditions: static system in accordance with the study method
-Photoperiod: 16 hours light/8 hours dark
-Test procedure: - cylindrical test vessels diameter 4.0 cm, height 6.5 cm; 20 ml test medium; not ventilated.
-Method of administration: Direct weighing
- Test concentration/s: 100 mg/L
- Solubilizer or other auxiliaries: ultra-turrax; magnetic stirrer; folded filter.
- Control: M4-Medium according to Elendt and BGA (1992)
- Water for dilution: M4-Medium according to Elendt and BGA (1992)
-Water hardness (M4-Medium): 14.7 °dH
-Test parameters during the test: oxygen [mg/L]; pH; water temperature [°C]; number of immobile daphnids after 24 hours and 48 hours

STUDY EVALUATION
-No. parallel solutions: 2
-Test volume: 20 mL
-No. daphnids per vessel: 10

Key result

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 99 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Nominal concentration test substance (mg/L)	Results after 24 and 48 hours
	Immobile daphnids				Oxygen (mg/L)  48 h	pH   48 h	Temperature (°C)  48 h
	Absolute 24 h	Absolute 48 h	% 24 h	% 48 h
Control 100	0 0	0 0	0 0	0 0	8.6 8.4	8.1 8.1	20.3 20.3

The values of the abiotic parameters (oxygen, pH and temperature) were determined from the total volume of the parallel solutions.

Analysis

Nominal concentration test substance (mg/L)	Results by start of test and after 48 hours (mg/L)
	0 hours	48 hours
Control	<2	2
100	56	54

Comments: all values refer to TOC determinations. According to the relevant product informations, 1mg/L TOC equals to 1.8 mg/L of the test substance (structural formula: C8H14)4; molecular weight: 174.22 g/mol)

Results

After 24 hours	After 48 hours
Nominal concentration	Arithmetic mean of analytical values
EC0: ≥ 100 mg/L	EC0: ≥ 99 mg/L

Comments relevant to result: the above test result refers to the concentration of the test substance which was directly calculated from analytically determined TOC values. According to the relevant product informations, 1mg/L TOC equals to 1.8 mg/L of the test substance (structural formula: C8H14)4; molecular weight: 174.22 g/mol)

Validity criteria fulfilled:

yes

Conclusions:

48 h EC0 ≥ 99 mg/L (arithmetic means of analytical values)

Executive summary:

48 h EC0 ≥ 99 mg/L (arithmetic means of analytical values) in a test carried out in accordance with "Acute Toxicity for Daphnia" Council Directive 92/69/EEC C.2 (1992) and OECD 202 "Daphnia sp. Acute Immobilisation Test".

Description of key information

48 h EC0 ≥ 99 mg/L (arithmetic means of analytical values) in a test carried out in accordance with "Acute Toxicity for Daphnia" Council Directive 92/69/EEC C.2 (1992) and OECD 202 "Daphnia sp. Acute Immobilisation Test".

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

In a study conducted in accordance with "Acute Toxicity for Daphnia" Council Directive 92/69/EEC C.2 (1992) and OECD 202 "Daphnia sp. Acute Immobilisation Test" the 48 h EC0 was determined to be greater than or equal to 99 mg/L based on arithmetic means of analytical values and greater than 100 mg/L based on nominal concentrations.