4-amino-6-chlorotoluene-3-sulphonic acid

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non GLP study according to national standards without analytical test item concentration verification

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1987

Report date:

1987

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: German Industrial Standard Test Guideline DIN 38412

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-amino-4-chloro-5-methyl-benzene sulfonic acid sodium salt

IUPAC Name:

2-amino-4-chloro-5-methyl-benzene sulfonic acid sodium salt

Details on test material:

- Name of test material (as cited in study report): Chlor-p-Toluidinsaeure Na-Salz
- Analytical purity: 99.5%

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Great waterflea
- Source: Originally obtained from the Institut national de Recherche Chimique Appliquee, France, since 1978 in-house culture
- Feeding during test: yes
- Food type: green algae culture
- Amount: starting on day 0 of the test with 5.8 mg/L CSB increasing until day 8 to 20 mg/L CSB. Afterwards daily 20 mg/L CSB. Data for a single daphnid parental animal
- Frequency: daily

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: offspring was counted using a particle counter (PC-320 , HIAC). Each particle greater than 350 µm were counted as a daphnid.

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Test conditions

Hardness:

2.5 ± 0.25 mM

pH:

7.71 - 8.24

Dissolved oxygen:

7.2 - 9.55 mg/L

Nominal and measured concentrations:

nominal: control, 15.625, 31.3, 62.5, 125, 250, 500, 1000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beakers
- Material, size, headspace, fill volume:glass, 50 mL fill volume
- Renewal rate of test solution (frequency/flow rate): on mondays, wednesdays and fridays
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated and char coal filtered tap water, acidity constant adjusted to 4.3 using H2SO4, hardness adjusted with distilled water
- Ca/mg ratio: 4:1
- Conductivity: 450 - 650 µSiemens/cm
- Intervals of water quality measurement: daily

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 16 : 8 hours light : dark
- Light intensity: 5 - 6 µEinstein (wavelength 400 - 750 nm)

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : survival of parent animals, number of offspring, reproduction rate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

21 d

Dose descriptor:

other: EC0

Effect conc.:

125 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Effect concentrations 2

Duration:

21 d

Dose descriptor:

other: EC0

Effect conc.:

125 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

- Mortality of parent animals: at the highest test concentration of 1000 mg/L 6 out of 10 daphnids
- Other biological observations: resting egg production in the higher test item concentrations