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EC number: 201-899-4 | CAS number: 89-33-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1988 - 1989
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: well performed OECD study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- not specified
- Principles of method if other than guideline:
- In the Closed Bottle Test the biodegradation is determined by measuring the O2 consumption of the bacteria and calculation of the ratio of it to the theoretical maximum of O2 demand during total combustion of the test substance. This method is used to test for ready biodegradability. It specifies that the amount of O2 consumed within 14 days after adaptation (when the 10% threshold is exceeded) and at the most 28 days overall must attain 60% of the measured COD (chemical oxygen demand) or of the calculated thOD (theoretical oxygen demand) of the test material in the test batches.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Ethyl 5-oxo-1-phenyl-2-pyrazoline-3-carboxylate
- EC Number:
- 201-899-4
- EC Name:
- Ethyl 5-oxo-1-phenyl-2-pyrazoline-3-carboxylate
- Cas Number:
- 89-33-8
- Molecular formula:
- C12H12N2O3
- IUPAC Name:
- ethyl 5-oxo-1-phenyl-4,5-dihydro-1H-pyrazole-3-carboxylate
- Details on test material:
- purity: 99.0 %
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Origin: Sewage treatment plant at Frankfurt am Main-Sindlingen
Type of inoculum: Supernatant from settled domestic sewage sludge
Content of suspended solids: Not determined
Amount used: One drop per one litre of the final volume of the batch
Pre-treatment: No pre-treatment
State of adaption: Not pre-adapted to the test substance or the reference substance. - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 50 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (O2 consumption)
- Value:
- 4
- Sampling time:
- 7 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 6
- Sampling time:
- 14 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 91
- Sampling time:
- 21 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 99
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- O2 consumtion
in % of the COD Time (d)
0 0
78 7
75 14
76 21
69 28
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The substance is readily biodegradable
- Executive summary:
The substance was tested for ready biodegradability according to OECD guideline 301 D (Closed Bottle Test) with activated, not adapted sludge from a domestic waste water treatment plant.
During a test period of 21 days the oxygen consumption achieved on average 91 % of the calculated oxygen demand for complete combustion of the added test substance in the batches. The adaptation phase took 14 days. It is defined as the time period until attainment of the 10 % threshold. The "14-days-window" for the biodegradation phase in the closed bottle test was kept. The total degradation period, defined as the time window from the 10 % threshold until 60 % were passed and the slope of the degradation curve was significantly lowered, took < 7 days.
Therefore, the substance has to be considered as readily biodegradable according to the OECD guideline 301 D.
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