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EC number: 700-774-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 10 May to XY July 2011
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: studiy performed according to OECD 301 B guideline and GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Absorbent N grade A
- IUPAC Name:
- Absorbent N grade A
- Details on test material:
- - Name of test material (as cited in study report): Absorbent N grade A
- Substance type: UVCB
- Physical state: Brown liquid
- Stability under test conditions: months
- Storage condition of test material: room temperature, in the dark
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Prior to use a sample of activated sludge from the sewage plant at Taunusstein-Bleidenstadt was washed twice with mineral nutrient solution of the CO2-Evolution-Test to eliminate organic components and carbonates from the sludge. After resolution with mineral nutrient medium the sludge was aerated by means of compressed humidified air for about four hours. Before use as inoculum for the CO2-Evolution-Test the sludge was homogenised in a "Waring Blender" at low speed for 2 minutes and then filtered through a cotton filter previously carefully rinsed with deionised water. The filtrate was used as inoculum (1% of the final volume of the test solution) on the same day of preparation.
- Duration of test (contact time):
- ca. 28 d
Initial test substance concentration
- Initial conc.:
- ca. 50.5 - 51.5 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: as mentioned in the guideline
- Additional substrate: none
- Solubilising agent: none
- Test temperature: Values were in the range of 20.6 to 23.7 °C (mean 21.8°C)
- pH: data not available
- pH adjusted: no
- Aeration of dilution water: the aeration rate of the test system was controlled at 4 L/h
- Continuous darkness: data not available
TEST SYSTEM
- Culturing apparatus: ≈5-litre amber carboys served as test vessels. They were closed with stoppers with tubing for gas inlet and outlet (gas exit line)
- Number of culture flasks/concentration: two vessel with the test item, one vessel with sodiumbenzoate (control item), one toxicity control
- Method used to create aerobic conditions: aeration
SAMPLING
- Sampling frequency: Titrations were performed at t3d, t5d, t10d, t14d, t20d, t28d, and t29d
- Sample storage before analysis: data not available
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Toxicity control: yes
STATISTICAL METHODS: not applicable
Reference substance
- Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 20 mg TOC/L
Results and discussion
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 5
- Sampling time:
- 28 d
- Remarks on result:
- other: mean value
- Details on results:
- Calculated from the organic carbon content of the test item and the measured CO2 generation, 0.5 % of the theoretical CO2 (ThCO2) has been generated by the test item within 28d of test period in the first culture with 51.5 mg of test item. 10.4 % of the theoretical CO2 (ThCO2) has been generated by the test item within 28d in the second culture with 50.5 mg of test item. The calculated mean degradation value of the test item was 5 %.
BOD5 / COD results
- Results with reference substance:
- The control item sodium benzoate was degraded 79 %, the "10-days-window" being met within 7 days (71 %).
Any other information on results incl. tables
Table 1: Results of Testing Biodegradability according to OECD 301 B – 1stTest Solution, Test Item
Date |
Time [d] |
1stTest Solution with»13 mg C/L |
% TCO2 (= % Biodegradation)3) |
|
mg CO2generated in the test solution, cumulative |
||||
03-Jun-2011 07-Jun-2011 10-Jun-2011 14-Jun-2011 21-Jun-2011 28-Jun-2011 29-Jun-2011 |
3 7 10 14 21 28 29 |
0.00 0.00 0.00 0.12 0.34 0.34 0.81 |
|
0.0 0.0 0.0 0.1 0.2 0.24) 0.55) |
1)TOC: Total Organic Carbon
2)ThCO2: Theoretical amount of CO2that can be generated by the test item
3)% TCO2: Percentage of total CO2the test item has generated in relation to the ThCO2
4) The test solution was stopped by the addition of 1 mL conc. HCl;
5) The difference between this titration and that before is due to the liberation of CO2after acidification of the test solutiTable 2: Results of Testing Biodegradability according to OECD 301 B - 2ndTest Solution, Test Item
Date |
Time [d] |
2ndTest Solution with»13 mg C/L |
% TCO2 (= % Biodegradation)3) |
|
mg CO2generated in the test solution, cumulative |
||||
03-Jun-2011 07-Jun-2011 10-Jun-2011 14-Jun-2011 21-Jun-2011 28-Jun-2011 29-Jun-2011 |
3 7 10 14 21 28 29 |
0.30 2.25 3.98 7.25 11.28 15.23 17.04 |
|
0.2 1.4 2.4 4.4 6.9 9.34) 10.45) |
1)TOC: Total Organic Carbon
2)ThCO2: Theoretical amount of CO2that can be generated by the test item
3)% TCO2: Percentage of total CO2the test item has generated in relation to the ThCO2
4) The test solution was stopped by the addition of 1 mL conc. HCl;
5) The difference between this titration and that before is due to the liberation of CO2after acidification of the test solution.Table 3: Results of Testing Biodegradability according to OECD 301 B – 3rdTest Solution, Sodium Benzoate
Date |
Time [d] |
3rdTest Solution with»22 mg C/L Sodium Bezoate |
% TCO2 (= % Biodegradation)3) |
|
mg CO2generated in the test solution, cumulative |
||||
03-Jun-2011 07-Jun-2011 10-Jun-2011 14-Jun-2011 21-Jun-2011 28-Jun-2011 29-Jun-2011 |
3 7 10 14 21 28 29 |
135.63 182.21 190.62 198.15 202.90 203.80 203.80 |
|
53 71 74 77 79 794) 795) |
1)TOC: Total Organic Carbon
2)ThCO2: Theoretical amount of CO2that can be generated by the test item
3)% TCO2: Percentage of total CO2the test item has generated in relation to the ThCO2
4) The test solution was stopped by the addition of 1 mL conc. HCl;
5) The difference between this titration and that before is due to the liberation of CO2after acidification of the test solution.
Table 4: Results of Testing Biodegradability according to OECD 301 B - 4thTest Solution, Toxicity Contro
Date |
Time [d] |
4thTest Solution with ≈20 mgC/L Sodium Benzoate +»12 mg C/L TS |
% TCO2 (= % Biodegradation)3) |
|
mg CO2generated in the test solution, cumulative |
||||
03-Jun-2011 07-Jun-2011 10-Jun-2011 14-Jun-2011 21-Jun-2011 28-Jun-2011 29-Jun-2011 |
3 7 10 14 21 28 29 |
121.41 164.79 177.80 192.16 216.00 217.89 221.72 |
|
29 40 43 46 52 524) 535) |
1)TOC: Total Organic Carbon
2)ThCO2: Theoretical amount of CO2that can be generated by the test item
3)% TCO2: Percentage of total CO2the test item has generated in relation to the ThCO2
4) The test solution was stopped by the addition of 1 mL conc. HCl;
5) The difference between this titration and that before is due to the liberation of CO2after acidification of the test solution.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: not readily biodegradable
- Conclusions:
- the test item may be regarded as “not readily biodegradable".
- Executive summary:
“Absorbent N grade A“was tested for biodegradability according to 'CO2-Evolution-Test' (OECD Guideline 301B). Calculated from the organic carbon content of the test item and the measured CO2generation, 0.5 % of the theoretical CO2(ThCO2) has been generated by the test item within 28d of test period in the first culture with 51.5 mg of test item. 10.4 % of the theoretical CO2(ThCO2) has been generated by the test item within 28d in the second culture with 50.5 mg of test item. The calculated mean degradation value of the test item was 5 %. Thus, the test item may be regarded as “not readily biodegradable". The control item sodium benzoate was degraded 79 %, the "10-days-window" being met within 7 days (71 %). The total CO2-evolution of the Blank was 93 mg CO2. Thus the test is regarded to be valid. The results obtained with the toxicity control indicate that there was no toxicity of the test item towards microorganisms at the concentration used within the test.
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