(methoxymethyl)triphenylphosphonium chloride

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2003

Report date:

2003

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

(methoxymethyl)triphenylphosphonium chloride

EC Number:

223-664-5

EC Name:

(methoxymethyl)triphenylphosphonium chloride

Cas Number:

4009-98-7

Molecular formula:

C20H20OP.Cl

IUPAC Name:

(methoxymethyl)triphenylphosphanium chloride

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

The sludge was kept under continuous aeration until further treatment. The concentration of suspended solids was 3.9 g/l in the concentrated sludge
(information obtained from the municipal sewage treatment plant). Before use, the sludge was allowed to settle (30-90 minutes) and the liquid
decanted for use as inoculum at the amount of 10 mI/I of mineral medium.

Duration of test (contact time):

29 d

Initial test substance concentration

Initial conc.:

14.5 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

Test duration
28 days (last CO2-measurement on the 29th day). During the test period aeration and stirring took place.
Test vessels
2 litre all-glass brown coloured bottles.
Milli-RO / Milli-Q water
Tap-water purified by reverse osmosis (Milli-RO) and subsequently passed over activated carbon and ionexchange cartridges (Milli-Q) (Millipore
Corp., Bedford, Mass., USA).
Sampling
0, 2, 5, 7, 9, 14, 19, 23, 27 and 29 days

Reference substance

Reference substance:

acetic acid, sodium salt

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (CO2 evolution)

Value:

0

Sampling time:

0 d

Remarks on result:

other: mean of bottle A and B

% Degradation 2

Parameter:

% degradation (CO2 evolution)

Value:

1

Sampling time:

5 d

Remarks on result:

other: mean of bottle A and B

% Degradation 3

Parameter:

% degradation (CO2 evolution)

Value:

1

Sampling time:

14 d

Remarks on result:

other: mean of bottle A and B

% Degradation 4

Parameter:

% degradation (CO2 evolution)

Value:

1

Sampling time:

23 d

Remarks on result:

other: mean of bottle A and B

% Degradation 5

Parameter:

% degradation (CO2 evolution)

Value:

1

Sampling time:

29 d

Remarks on result:

other: mean of bottle A and B

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

MMC was not readily biodegradable under the conditions of the modified Sturm test presently performed.