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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
17th December 2012 to 20th December 2012
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study (DIN 38412) with acceptable restrictions.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2012
Report date:
2012

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
ISO 8692 (Water Quality - Fresh Water Algal Growth Inhibition Test with Scenedesmus subspicatus and Selenastrum capricornutum)
Deviations:
no
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
3-acetoxymethylen-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
EC Number:
213-485-0
EC Name:
3-acetoxymethylen-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
Cas Number:
957-68-6
Molecular formula:
C10H12N2O5S
IUPAC Name:
(6R,7R)-3-[(acetyloxy)methyl]-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder
Details on test material:
Name: 7-Aminocefalosporansäure (7-ACA)
Lot no.: B146988

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes
Details on test solutions:
A stock solution of the test item was prepared in water and diluted with water to concentrations between 138 mg/l and 440 mg/l.

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
Stock no.: Chodat, 86.81 SAG
Preparatory culture: the preparatory culture was incubated for 72 hours under the same test conditions as the test cultures.
Seeding of the preparatory culture: After the incubation time the cell density of the preparatory culture was adjusted to 10E05 cells/ml. 10 ml were added to each test preparation.

Study design

Test type:
static
Water media type:
not specified
Limit test:
no
Total exposure duration:
72 h
Post exposure observation period:
The cell density was measured after 24, 48 and 72 hours.

Test conditions

Test temperature:
23 +/- 2°C
pH:
In the test preparations and controls: between 8.1 and 9.4
Nominal and measured concentrations:
Nominal: 138, 182, 275, 369 and 440 mg/l
Details on test conditions:
The tests were performed with independent test preparations of different concentrations.

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
390 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
55 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The EbC50 value of 7-aminocephalosporanic acid is: 100 mg/l < ErC50 value < 1000 mg/l