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EC number: 203-818-8 | CAS number: 110-95-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study with acceptable restrictions (occlusive treatment)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 982
- Report date:
- 1982
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Deviations:
- yes
- Remarks:
- (occlusive treatment)
- GLP compliance:
- not specified
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- N,N,N',N'-tetramethyltrimethylenediamine
- EC Number:
- 203-818-8
- EC Name:
- N,N,N',N'-tetramethyltrimethylenediamine
- Cas Number:
- 110-95-2
- Molecular formula:
- C7H18N2
- IUPAC Name:
- N,N,N',N'-tetramethyltrimethylenediamine
- Details on test material:
- - Name of test material (as cited in study report): Tetramethylpropandiamin-1,3
- Analytical purity: 99%
- Physical state: liquid
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Dr. K. Thomae GmbH, Biberach, Germany
- Age at study initiation: about 12 weeks
- Mean weight at study initiation: 212 g (males); 190 g (females)
- Housing: housing of individual animals during the exposure period (24 hours). lf there are no lesions afterward, the animals are kept in groups.
- Diet: Ssniff R; Ssniff Versuchstierdiaeten, Soest, Germany; ad libitum
- Water: fulIy demineralized water ad libitum each workday; tap water ad libitum on public holidays
- Acclimation period: at least 1 week.
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20-26 °C
- Humidity (%): 45-75 %
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- water
- Details on dermal exposure:
- TEST SITE
- Area of exposure: 50 cm²
REMOVAL OF TEST SUBSTANCE
- Washing (if done): warm water
- Time after start of exposure: 24 h
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 10 ml
- Concentration (if solution): 50%, 10% or 4.64% - Duration of exposure:
- 24 h
- Doses:
- 2500, 1000 or 464 mg/kg bw
- No. of animals per sex per dose:
- 5
- Control animals:
- not specified
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations: Recording of signs and symptoms <15min, 15min, 30min, 1h, 2h, 4h and 5h after test substance administration and then once each workday. Check for moribund and dead animals twice each workday and once daily at weekends and on public holidays.
- Frequency of weighing: day 0, 2, 7 and 13
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- ca. 1 180 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- > 1 000 - < 2 500
- Remarks on result:
- other: 3/10 animals died within 7 days in the 1000 mg/kg bw dose group; LD50 determined by interpolation
- Mortality:
- - 2500 mg/kg bw: all animals died within 24 h.
- 1000 mg/kg bw: 1 male and 1 female died within 48 h and 1 further female died within 7 days.
- 464 mg/kg bw: no animal died within the observation period. - Clinical signs:
- other: Symptoms: dyspnoea, apathy, excitation, spastic gait, poor general state (see details in table in remarks on results) Local findings: erythema, necrosis, ruptured necrosis, edema, scaling, anaemia, scar formation (see details in table in remarks on result
- Gross pathology:
- Animals that died: application site: severe hyperemia of muscles and subcutis; peritoneum blunt; general passive hyperemia.
Sacrificed animals: several animals with scabbed, extensive necroses at the margin of the application area; striated necroses in isolated animals.
Any other information on results incl. tables
Mortality:
Dose (mg/kg) |
Gender |
Conc. (%) |
1 h |
24 h |
48 h |
day 7 |
day 14 |
2500 |
male |
50 |
0 |
5 |
5 |
5 |
5 |
2500 |
female |
50 |
0 |
5 |
5 |
5 |
5 |
1000 |
male |
10 |
0 |
0 |
1 |
1 |
1 |
1000 |
female |
10 |
0 |
0 |
1 |
2 |
2 |
464 |
male |
4.64 |
0 |
0 |
0 |
0 |
0 |
464 |
female |
4.64 |
0 |
0 |
0 |
0 |
0 |
Weight: (g)
Dose (mg/kg) |
Gender |
day 0 |
day 2 |
day 7 |
day 13 |
2500 |
male |
207 |
- |
- |
- |
1000 |
male |
215 |
197 |
223 |
271 |
464 |
male |
214 |
203 |
241 |
277 |
2500 |
female |
186 |
- |
- |
- |
1000 |
female |
192 |
181 |
191 |
217 |
464 |
female |
194 |
186 |
204 |
216 |
Signs and symptoms:
Symptoms: |
2500 mg/kg bw |
1000 mg/kg bw |
464 mg/kg bw |
|||
|
male |
female |
male |
female |
male |
female |
Dyspnoea |
15 min - 4 h |
15 min - 4 h |
30 min - 6 d |
30 min - 6 d |
30 min - 5 d |
30 min - 5 d |
Apathy |
15 min - 4 h |
15 min - 4 h |
30 min - 6 d |
30 min - 6 d |
30 min - 5 d |
30 min - 5 d |
Excitation |
< 15 min |
< 15 min |
- 15 min |
- 15 min |
- 15 min |
- 15 min |
Spastic gait |
15 min - 4 h |
15 min - 4 h |
2 d |
2 d- 5 d |
2 d |
2 d |
Aggressiveness |
- |
- |
- |
2 d |
- |
- |
Poor general state |
< 15 min - 4 h |
< 15 min - 4 h |
30 min - 6 d |
15 min - 6 d |
30 min - 5 d |
30 min - 5 d |
min: minutes; h: hours; d: day
Local findings:
Dose (mg/kg) |
2500 |
1000 |
464 |
Erythema |
- |
7 d -13 d |
7 d - 13 d |
Necrosis |
- |
1 d - 13 d |
1 d - 13 d |
Ruptured necrosis |
- |
13 d |
- |
Edema |
- |
1 d - 13 d |
1 d - 7 d |
Scaling |
- |
7 d - 13 d |
7 d |
Anaemia |
- |
1 d |
1 d |
Scar formation |
- |
- |
13 d |
h: hours; d: day
Applicant's summary and conclusion
- Executive summary:
The acute dermal toxicity of N,N,N',N'-tetramethylpropane-1,3-diamine (TMPDA) was evaluated in rats according to OECD N° 402 guideline. TMPDA was applied to the skin of groups of 5 male and 5 female Wistar rats at doses of 464, 1000 and 2500 mg/kg under an occlusive dressing for 24 hours. The application area was then washed with warm water. Following treatment, rats were observed daily and weighted weekly. A gross necropsy examination was performed at the time of scheduled euthanasia (Day 14). At 2500 mg/kg, all animals died within 24 h. At 1000 mg/kg, 1 male and 1 female died within 48 h and 1 further female died within 7 days. Systemic clinical signs observed were: dyspnea, apathy, excitation, spastic gait, poor general state. Local effects observed were: erythema, necrosis, ruptured necrosis, edema, scaling, anemic, scar formation. Animals found dead showed severe hyperemia of muscles and subcutis, peritoneum blunt and general passive hyperemia at the application site. Sacrificed animals showed scabbed, extensive necroses at the margin of the application area, striated necroses in isolated animals. Under these experimental conditions, the dermal LD50 of TMPDA is 1180 mg/kg in male and female Wistar rats.
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