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EC number: 620-365-5 | CAS number: 9016-72-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1998/05/04 to 1998/10/01
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 1992
- Deviations:
- yes
- Remarks:
- Although the analytical results are partly less than 80 % of nominal the acute toxicity is based on nominal concentrations.
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Remarks:
- HPLC-UV
- Details on sampling:
- The samples were taken at the end of the test and at test termination. The samples were stored in a refrigerator until they were analysed.
- Vehicle:
- no
- Details on test solutions:
- Test concentrations were 0.125, 0.25, 0.50, 1.00, 2.00 and 4.00 mg a.s./L (nominal).
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- All test fish were held in culture tanks on a 16/8 hour light/dark photoperiod and observed for at least 14 days prior to testing. Less than 3 % mortality was noted prior to the test initiation and all unsuitable fish (e.g. injured, deformed, etc.) were eliminated from inclusion in the test prior to assignment to test groups. A continuous record offish observations during the holding period, along with any prophylactic and therapeutic disease treatments, if applicable, is included in the raw data of the fish lot.
During the acclimation period they were fed a commercial trout diet. They were not fed 48 h before and during the study. The feed was not analyzed for unwanted contaminants. In this test mean body wet weight of the fish at the beginning of the test was 0.7 ± 0.1 g (Mean ±SD), mean body total length was 4.2 + 0.2 cm (Mean + SD). The biomass loading was 0.18 g fish/L test medium. - Test type:
- static
- Water media type:
- other: Reconstituted water prepared by adding salt stock solutions to demineralized water
- Total exposure duration:
- 96 h
- Hardness:
- 40 - 60 mg CaCO3/L
- Test temperature:
- 12.3°C - 12.5°C
- pH:
- 7.0 - 7.3
- Dissolved oxygen:
- 97% - 101%
- Nominal and measured concentrations:
- Nominal concentrations: 0.125, 0.25, 0.50, 1.00, 2.00 and 4.00 mg a.s./L, Mean measured concentrations: 0.082, 0.182, 0.368, 0.723, 1.29 and 2.54 mg a.s./L
- Details on test conditions:
- The aquaria were made of glass and had a size of 32 x 36 x 38 cm (l x d x h). The test volumes
amounted to 40 L. For every test concentration one aquarium was used, labelled with study number, a consecutive number and the nominal concentration of the test substance. The aquaria were placed in a temperature controlled room. Immediately before the start of the test water samples were taken from the center of the aquaria for analytical determination of the active ingredient concentration.
At the start of the test, ten fish were introduced into each aquarium in an order determined by a table of random numbers. The time between preparing the test solution and the addition of fish were 30 min. During the test, fish were examined after four hours and then daily for mortalities and symptoms of intoxication. In case 100% mortality was reached in test concentrations prior to the end of the test, the analytical determinations were made at those times. - Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 329 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.103 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.206 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- Study was performed with the formulated product Antracol U with a active substance content of 82.3%.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- An acute 96 h toxicity test was conducted to estimate the toxicity of the test substance to Rainbow Trout (Oncorhynchus mykiss). Ten fish per test concentration were exposed to nominal concentrations of 0.125, 0.25, 0.50, 1.00, 2.00 and 4.00 mg a.s./L. Based on nominal concentrations of propineb, the LC50 (96 h) was determined to be 0.40 mg a.s./L.
- Executive summary:
An acute 96 h toxicity test was conducted to estimate the toxicity of the test substance to Rainbow Trout (Oncorhynchus mykiss). The primary measure for acute toxicity was mortality. Sublethal and behavioural observations were made during the course of the study. Ten fish per test concentration were exposed to nominal concentrations of 0.125, 0.25, 0.50, 1.00, 2.00 and 4.00 mg a.s./L.
In test water mean measured values for propineb between 77% and 89% of nominal were found. There were neither symptoms of intoxication nor mortality in the control group. The lowest lethal concentration (LLC) was 0.50 mg a.s./L. The lowest-observed-effect concentration (LOEC) was 0.25 mg a.s./L. The minimum concentration causing 100% mortality (96 h) was 1.00 mg a.s./L. The no-observed-effect-concentration (NOEC) was 0.125 mg a.s./L. Based on nominal concentrations of propineb, the LC50 (96 h) was determined to be 0.40 mg a.s./L.
Reference
Description of key information
An acute 96-h toxicity test was conducted to estimate the toxicity of propineb to Rainbow Trout (Oncorhynchus mykiss). Ten fish per test concentration were exposed to nominal concentrations of 0.125, 0.25, 0.50, 1.00, 2.00 and 4.00 mg a.s./L. This study was conducted according to OECD 203. Based on nominal concentrations of propineb, the LC50 (96 h) was determined to be 0.329 mg a.s./L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Remarks:
- mortality
- Effect concentration:
- 0.329 mg/L
Additional information
In addition, a study by Dogerloh (2004) as available. Test conditions met all validity criteria, given by the mentioned guidelines. The LC50 (96 h) was determined to be >66.7 mg a.s./L.
A second additional study by Bruns (2011) is available. The test conditions met all validity criteria, given by the mentioned guidelines. The LC50 (96 h) was determined to be 4.73 mg a.s./L.
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