Dehydratase, carbonate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Study period:

1995-02-13 to 1995-06-28

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Due to the similarity between the two enzymes, similar results are expected for carbonic anhydrase.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

Certificate included in the report

Test material

Test material information

Constituent 1

Reference substance name:

Active enzyme protein of alpha-amylase (EC no. 232-565-6, CAS no. 9000-90-2, EC name: Amylase, alpha-, Enzyme Class No.: 3.2.1.1)

Molecular formula:

Not available

IUPAC Name:

Active enzyme protein of alpha-amylase (EC no. 232-565-6, CAS no. 9000-90-2, EC name: Amylase, alpha-, Enzyme Class No.: 3.2.1.1)

Constituent 2

Reference substance name:

Protein as a constituent of enzyme deriving from the fermentation or extraction process

Molecular formula:

Not available

IUPAC Name:

Protein as a constituent of enzyme deriving from the fermentation or extraction process

Constituent 3

Reference substance name:

Inorganic salts as a constituent of enzyme deriving from the fermentation or extraction process

Molecular formula:

Not available. See remarks.

IUPAC Name:

Inorganic salts as a constituent of enzyme deriving from the fermentation or extraction process

Constituent 4

Reference substance name:

Carbohydrates constituent of enzyme deriving from the fermentation or extraction process

Molecular formula:

Not available. See remarks.

IUPAC Name:

Carbohydrates constituent of enzyme deriving from the fermentation or extraction process

Constituent 5

Reference substance name:

Lipids as a constituent of enzyme deriving from the fermentation or extraction process

Molecular formula:

Not available. See remarks.

IUPAC Name:

Lipids as a constituent of enzyme deriving from the fermentation or extraction process

Test material form:

solid: particulate/powder

Details on test material:

- Lot/batch No.: PPY 5067
- Expiration date of the lot/batch: 1997-01-01

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Domestic waste water treatment plant : ARA Ergolz II, Füllinsdorf, Switzerland.
- Preparation of inoculum for exposure: The sludge was washed three times with tap water and an amount corresponding to 4 g/L dry material (± 10%) was mixed with Sörensen buffer solution pH7 and then aerated prior to incubation. An amount of 0.5 mL sludge (filtered over cotton wool) was added to 1000 mL test medium.

Duration of test (contact time):

ca. 28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

44 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

>= 18.2 - <= 19 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

TEST CONDITIONS
- Composition of medium: according to OECD Guideline No. 301 E
- Test temperature: 19.7 - 21.5°C
- Continuous darkness: yes

TEST SYSTEM
- Number of culture flasks/concentration: 2

SAMPLING
- Sampling frequency: day 0 (treatment day), 7, 14, 21, 27 and 28
- Sampling method: Per sampling interval, two flasks of the samples containing the test article or the reference compound, one flask of the inoculum blank and one flask of the toxicity control were taken and analysed for DOC in triplicate.

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Toxicity control: Aniline and the test compound were dissolved in the test medium at concentrations of 45 mg/L SP 703 and 26 mg/L aniline corresponding to an actually measured concentration of 39.3 mg DOC/L

Reference substance

Reference substance:

aniline

Results and discussion

Preliminary study:

Test substance concentration was based on the DOC-values from a pre-test.

% Degradation

% Degradation 1

Key result

Parameter:

% degradation (DOC removal)

Value:

99

Sampling time:

28 d

% Degradation 2

Key result

Parameter:

% degradation (DOC removal)

Value:

95

Sampling time:

7 d

BOD5 / COD results

Results with reference substance:

The reference compound aniline reached complete biodegradation during the first seven exposure days. At the end of the test, aniline was readily biodegraded by an average of 95.1%.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

Alpha-amylase was found to be readily biodegradable in the 'Modified OECD Screening Test' (OECD 301E).

Executive summary:

Alpha-amylase was tested for ready biodegradability in the '28 -day Modified OECD Screening Test'. The test was performed according to OECD 301E and in compliance with GLP.

The concentration of DOC (Disssolved Organic Carbon) in the test flasks containing alpha-amylase decreased by an average of 95.0 %, 92.5 % and 93.8% within 7, 14 and 21 days of exposure, respectively. At the end of the test (day 28), alpha-amylase was biodegraded by an average of 99.0%. Consequently, alpha-amylase was found to be readily biodegradable.

The reference compound Aniline reached complete biodegradation during the first seven exposure days. At the end of the test (day 28) Aniline was readily biodegraded by an average of 95.1%.