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EC number: 224-226-6 | CAS number: 4253-90-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- May 2000
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Procedures: 100 mg of TPS 44 were introduced in 1 litre of dilution water, vigorously shaken at 30°C for 4 hours then at 20°C for 20 hours (preliminary assay) or 62 hours (main study). Daphnids were exposed to a concentration range of 0.073 to 0.270 mg/l forming a geometric progression with a factor of 1.3. After 24 hours exposure a count of immobile Daphnia was carried out and another count was taken 48 hours after the start of the test. After decantation, the saturated solution was filtered on a 0.22µm filter. pH and dissolved oxygen were measured in the highest concentration and the control at the start of the test and in all concentrations at the end of the test.
- Vehicle:
- not specified
- Details on test solutions:
- The saturated stock solution was diluted using ultrapure water to produce concentrations ranging from 13.5 - 50% (vol). Concentrations were then produced in a geometric progression with a ratio of 1.3. The concentrations of the test substance were maintained to within 80% of the initial concentration throughout the duration of the test. Dilution water was prepared in the laboratory using pure water and salts according to ISO 6341.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Strain: Daphnia magna straus strain 5 or strain A. Source/supplier: Breeding colony was realized in the laboratory, organisms are selected by sieving and held in Elendt M4 media. Breeding method: Not available. Age: Less than 24 hours. Feeding: Microscopic algae Raphidocelis subcapitata. Pretreatment: No. Feeding during test: No. Control group: Yes.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- No data reported
- Hardness:
- According to ISO 6341 medium
- Test temperature:
- Test temperature: 19.5 +/-1°C.
- pH:
- 7.75 - 7.84
- Dissolved oxygen:
- 9.0 - 9.7 mg/l.
- Salinity:
- According to ISO 6341 medium
- Nominal and measured concentrations:
- Nominal: 13.5 - 50 (volume saturated). Nominal concentrations: 13.5, 17.5, 22.8, 29.6, 38.5, 50 % saturated solution volume. Initial measured concentrations: 0.073 - 0.270 mg/l (extrapolated from measured concentrations). Daphnids were exposed to a concentration range of 0.073 to 0.270 mg/l forming a geometric progression with a factor of 1.3.
- Details on test conditions:
- The test was conducted in darkness in tightly closed 120 ml vessels, with 5 organisms per vessel and 4 replicates per concentration.
- Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.27 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- not specified
- Basis for effect:
- not specified
- Remarks on result:
- other: no data available
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.24 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- not specified
- Basis for effect:
- not specified
- Remarks on result:
- other: no data available
- Details on results:
- No data reported
- Results with reference substance (positive control):
- 24 hour EC50: 1.5 mg/l
- Reported statistics and error estimates:
- Confidence intervals: 24 hour EC50: not determined; 48 hour EC50: 0.16 - 21.
- Validity criteria fulfilled:
- yes
- Conclusions:
- In an ecotoxicity test using Daphnia magna the 24 hour EC50 for polysulfides, di-tert-butyl was > 0.27 mg/l and the 48 hour EC50 was 0.24 mg/l.
- Executive summary:
Acute toxicity of TPS44 to Daphnia magna was determined according to OECD TG 202 protocol following GLP. Daphnids were exposed to a graded series of concentrations up to TPS44 water solubility limit (range from 0.073 to 0.270 mg/L with a 1.3 spacing factor). Static exposure was performed in quadruplicates (5 daphnids in each) for 48 hour at 19.5°C in the dark. Daphnid immobilisation was checked after 24 and 48 hour. The study was conducted in hermetically sealed flasks with no head-space. TPS 44 concentrations were determined at the beginning and the end of the test in all treatments. Toxicity was calculated according to the measured concentrations. 48h-EC50 was 0.24 mg/L.
Reference
Table 1: % of immobilisation in each treatment after 24h and 48h exposure to the test substance.
Mean measured concentration (mg/L) |
Immobilisation % |
|
24 h |
48 h |
|
0 |
0 |
0 |
<DL |
0 |
20 |
<DL |
5 |
10 |
<DL |
0 |
35 |
0.16 |
10 |
40 |
0.21 |
5 |
35 |
0.27 |
30 |
60 |
Description of key information
In an ecotoxicity test using Daphnia magna the 24 hour EC50 for polysulfides, di-tert-butyl is > 0.27 mg/l and the 48 hour EC50 is 0.24 mg/l.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.24 mg/L
Additional information
Thiebaud (2000) is a 48 -hour, static, freshwater, GLP-compliant study on Daphnia magna with analytical monitoring, following OECD TG 202. The study is considered reliable and fully adequate for assessment. The EC50 result for aquatic invertebrates of 0.24 mg/l indicates that polysulfides, di-tert-bu would be 'very toxic to aquatic organisms'.
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