Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 217-199-7 | CAS number: 1772-25-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Key value for chemical safety assessment
Skin sensitisation
Link to relevant study records
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2022.09.05-2022.09.26
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 442B (Skin sensitisation: Local Lymph Node Assay: BrdU-ELISA or –FCM)
- GLP compliance:
- yes (incl. QA statement)
- Type of study:
- mouse local lymph node assay (LLNA): BrdU-ELISA
- Species:
- mouse
- Strain:
- CBA:J
- Sex:
- female
- Details on test animals and environmental conditions:
- TEST ANIMALS
- Source:KOATECH_Korea
- Females (if applicable) nulliparous and non-pregnant: yes
- Microbiological status of animals, when known: NONE
- Age at study initiation: 9 weeks
- Weight at study initiation: 21.2-25.6g
- Housing:Rodents room
- Diet (e.g. ad libitum):libitum
- Water (e.g. ad libitum):libitum
- Acclimation period:The animals of receipt were quarantined for more than 3 days in quarantine room, and acclimated to the laboratory conditions for more than 5 days. All animals were observed once a day for general symptoms and only healthy animals were used in this study.
ENVIRONMENTAL CONDITIONS
- Temperature (°C):21.2 ~ 23.7 ºC
- Humidity (%):49.6 ~ 57.4 %
- Air changes (per hr):10 - 15 air exchanges / hour
- Photoperiod (hrs dark / hrs light):12 hours (8 AM light on ~ 8 PM light off ) - Vehicle:
- dimethylformamide
- Remarks:
- SIGMA-ALDRICH, STBK2956
- Concentration:
- The preliminary test and the main test were prepared at a concentration of 100%, 50% (v/v) and 25% (v/v).
- No. of animals per dose:
- 4
- Positive control substance(s):
- eugenol (CAS No 97-53-0)
- Parameter:
- SI
- Value:
- 2.439
- Test group / Remarks:
- positive control group
- Parameter:
- SI
- Value:
- 1.314
- Test group / Remarks:
- Test substance (100 %(v/v))
- Parameter:
- SI
- Value:
- 1.174
- Test group / Remarks:
- Test substance (50 %(v/v))
- Parameter:
- SI
- Value:
- 1.128
- Test group / Remarks:
- Test substance (25 %(v/v))
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The stimulation index (SI) in animals treated with vehicle control group, 100 %, 50 % and 25 %(v/v) concentrations of test substance and positive control group were calculated as 1.000, 1.314, 1.174, 1.128 and 2.439, respectively. The SI obtained with 1,3,6-Hexanetricarbonitrile concentrations up to 100 % in the present study were below 1.6 and, therefore, support non-sensitizing properties of 1,3,6-Hexanetricarbonitrile when tested in a positive control group.
- Executive summary:
This study was performed to evaluate skin sensitization of 1,3,6-Hexanetricarbonitrile in female CBA/J mouse. All animals were observed once daily for clinical signs and mortality throughout the study period. Body weights were recorded on Day 1 and Day 6. On Day 5, 25 μL of test solution was applied to the dorsum of both ears of a mouse daily for 3 consecutive days. 0.5 mL of BrdU solution was administered intra-peritoneally. Approximately 24 hours (Day 6) after BrdU solution injection, euthanasia was performed with CO2 gas. After that, excised the draining auricular lymph nodes from each mouse ear, and the lymph nodes were subjected to cell proliferation and absorbance was measured at the ELISA reader.
The results were as follows.
1) No death of animal was observed during the study period.
2) No clinical signs related to the test substance administration were observed.
3) No abnormal body weight changes were observed in preliminary or main tests.
4) No abnormal ear thickness changes were observed in preliminary or main tests.
5) No abnormal local skin irritation changes were observed in preliminary or main tests.
6) The stimulation index in animals treated with vehicle control group, 100 %, 50 % and 25 %(v/v) concentrations of test substance and positive control group were calculated as 1.000, 1.314, 1.174, 1.128 and 2.439, respectively. The SI obtained with 1,3,6-Hexanetricarbonitrile concentrations up to 100 % in the present study were below 1.6 and, therefore, support non-sensitizing properties of 1,3,6-Hexanetricarbonitrile when tested in a positive control group.
As a result, the test substance(1,3,6-Hexanetricarbonitrile) was not a dermal sensitizer in the Local Lymph Node Assay.
The concentrations according to purity of test substances 1,3,6-Hexanetricarbonitrile 100 %, 50 % (v/v) and 25 % (v/v) were 99.67 %, 49.835 %, and 24.9175 %.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not sensitising)
Respiratory sensitisation
Endpoint conclusion
- Endpoint conclusion:
- no study available
Justification for classification or non-classification
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.