Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 876-151-9 | CAS number: 2292123-68-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- April 13, 2004
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: "Daphnia sp. Acute immobilization test" - Testing method for New Chemical Substances of Japanese Chemical Substances Control Law
- Version / remarks:
- July 1, 2019
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guidance Document No. 23
- Version / remarks:
- February 2019
Guidance Document on Aqueous-phase Aquatic Toxicity Testing of Difficult Test Chemicals - GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: single concentration (nominal concentration: 100 mg/L)
- Sampling method: Solution sampled separately from the preparation container (at start of exposure); mixed solution was taken out with equal volumes from the middle layer of the test solution in the test vessels in each test level (at the end of exposure)
- Sample storage conditions before analysis: samples were analysed straightaway after collection. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A saturated solution was prepared. 100g test item and 1000mL dilution water were mixed to produce the test concentration 100 mg/L and stirred by a magnetic stirrer for 48h at approx. 20°C to prepare the test solution. The test solution was divided into each test vessel.
- Controls: Dilution water (negative control) prepared using the same method as the test solution but no test item added
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): No used; not applicable
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): not applicable
- Test concentration separation factor: not applicable (limit test)
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): yes: white suspension, precipitation and insoluble matter were observed.
- Other relevant information: undissolved test item had no physical effect on the test organisms - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia manga (water flea)
- Strain/clone: Clone A
- Justification for species other than prescribed by test guideline: Species recommended by test guideline
- Age at study initiation (mean and range, SD): Less than 24 hours
- Method of breeding: Young daphnids produced by parents in the test facility. The parents to obtain the young daphnids were bred in the same quality of water as used in the study.
- Source: University of Sheffield, UK
- Age of parental stock (mean and range, SD): 21 days old
- Feeding during test: no
- Food type: not applicable
- Amount: not applicable
- Frequency: not applicable
ACCLIMATION
- Acclimation period: not specified
- Acclimation conditions (same as test or not): yes
- Type and amount of food: Chlorella vulgaris 0.1-0.2 mgC (organic carbon content)
- Feeding frequency: once per day
- Health during acclimation (any mortality observed): not reported - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- As recommended in the guideline
- Post exposure observation period:
- Not applicable.
- Test temperature:
- 20°C
- pH:
- 7.9
- Dissolved oxygen:
- 8.7 mg/L at start of exposure
8.5 mg/L at end of exposure - Nominal and measured concentrations:
- Nominal: 100 mg/L
Measured: 0.00124 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL glass beaker
- Type (delete if not applicable): open - transparent plastic lid
- Volume of solution: 100 mL
- Aeration: no
- No. of organisms per vessel: 5 daphnids/vessel
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water, aerated sufficiently and temperature controlled
- Total organic carbon: <0.5 mg/L
- Particulate matter: <1 mg/L
- Metals:- Pesticides: - Chlorine: <0.02 mg/L
- Alkalinity: 39 mg/L
- Ca/mg ratio: 44 mg/L
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: Room light, 16-hour light, 8 hours dark
- Light intensity: not reported
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilisation, 24 hours and 48 hours after exposure. Immobilisation considered when daphnids not able to swim for 15 seconds after gentle agitation of the test vessel
VEHICLE CONTROL PERFORMED: no - no vehicle used
RANGE-FINDING STUDY
- Test concentrations: 100 mg/L (saturated solution)
- Results used to determine the conditions for the definitive study: yes. - Reference substance (positive control):
- yes
- Remarks:
- Not conducted in parallel with study but lab conducts positive controls periodically to confirm the reproducibility of the test system. Period of study - April 16 - April 18 48h EC50 = 0.22 mg/L
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks on result:
- other: No immobilisation was observed during the test.
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.001 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Remarks on result:
- other: Limit of solubility of the test item. No immobilisation was observed during the test.
- Details on results:
- - Other adverse effects control: no
- Immobilisation of control: no
- Abnormal responses:
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no effects observed - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: 48h EC50 = 0.22 mg/L
- Limit test: yes - Reported statistics and error estimates:
- None
- Validity criteria fulfilled:
- yes
- Remarks:
- Dissolved oxygen was >3 mg/L at the end of exposure
- Conclusions:
- No immobilisation was observed during the limit test. The 48h EC50 was calculated as >0.00124 mg/L greater than the solubility of the test item in the test water.
Reference
Table 1: Immobility results
Measured concentrationa (mg/L) | Vessel | 24hours | 48 hours | ||
No. of immobilised daphnids/Total daphnids | Immobility (%) | No. of immobilised daphnids/Total daphnids | Immobility (%) | ||
Control | A | 0 / 5 | 0 | 0 / 5 | 0 |
B | 0 / 5 | 0 / 5 | |||
C | 0 / 5 | 0 / 5 | |||
D | 0 / 5 | 0 / 5 | |||
0.00124 | A | 0 / 5 | 0 | 0 / 5 | 0 |
B | 0 / 5 | 0 / 5 | |||
C | 0 / 5 | 0 / 5 | |||
D | 0 / 5 | 0 / 5 |
aTime-weighted mean of the measured concentrations (also expressed as mesured concentration in the following tables)
Table 2: Observed abnormal response
Measured concentration (mg/L) | Observed abnormal response | |
24 hours | 48 hours | |
Control | - | - |
0.00124 | - | - |
Table 3: EC50 values to Daphnia magna
Exposure duration | EC50 (mg/L) |
24 hours | >0.00124 |
48 hours | >0.00124 |
Table 4: Measured concentration of the test item in test solution
Nominal concentration (mg/L) | Measured concentration (mg/L) (percentage of measured concentration versus that at the start %) | ||
At the start | At the end | Time-weighted average mean | |
Control | <LOQ | <LOQ | - |
100 | 0.000360 | 0.00299 (830) | 0.00124 |
LOQ = 0.0002 mg/L
The time weighted mean is calculated by the following calculation:
[48 (C0-C48)/(lnC0-lnC48]/48
Cn: the measured concentration at n hours
lnCn: the natural logarithm of Cn
Description of key information
In a study conducted in accordance with OECD Guideline 202 on Daphnia Magna, the 48h EC50 >100 mg/L (nominal) was reported. Based on measured concentrations, no immobilisation was observed up to the limit of solubility of the test item (TWA measured with analytical verification at 0.00124 mg/L).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- > 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.