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EC number: 433-460-1 | CAS number: 210880-92-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 29 Jul - 15 Sep, 1999
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 216 (Soil Microorganisms: Nitrogen Transformation Test)
- Version / remarks:
- 1999
- Deviations:
- yes
- Remarks:
- Lucerne ratio C/N specified in the report. According to the current OECD guideline 216: The formation rate in mg/kg dws/day is not reported
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 217 (Soil Microorganisms: Carbon Transformation Test)
- Version / remarks:
- 1999
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on preparation and application of test substrate:
- Sandy Loam soil was used, with a sand content between 50-75%, organic carbon 0.5-1.5% and pH of 5.5-7.5.
Soil samples corresponding to 1.2 kg soil at 40% MWHC were weighed into 2.5 L capacity plastic containers.
A stock solution was prepared by reconstituting 506 mg of test material in a total volume of 250 mL of distilled-grade water. A 10.0 mL aliquot of the stock solution was diluted to a total volume of 100 mL with distilled-grade water to give a treatment solution concentration of 0.100 mg a.i./mL, equivalent to the 5x rate. A 2.0 mL aliquot of the stock solution was diluted to a total volume of 100 mL with distilled-grade water to give a treatment solution concentration of 0.020 mg a.i./mL, equivalent to the 1x rate.
Soil replicates were treated by evenly dispensing 12.0 mL aliquots of the treatment solution over a thin layer of soil using a glass pipette. Droplet size was as small as possible and the soil was thoroughly mixed during and after treatment. Untreated soil controls were prepared by adding 12.0 mL distilled-grade water to soil in the same manner as that applied to treated soils. After treatment, soil samples in the nitrogen transformation test were amended with 0.5% (w/w) lucerne meal and thoroughly mixed. - Test organisms (inoculum):
- soil
- Total exposure duration:
- 28 d
- Test temperature:
- 20 ± 2°C
- Moisture:
- 40% MWHC
- Organic carbon content (% dry weight):
- 1.1
- Nitrogen content (% dry weight):
- 0.16
- Details on test conditions:
- Containers were sealed with snap-top lids containing holes to maintain aeration and incubated in the dark at 20 ± 2°C for 6 days to pre-equilibrate soil to laboratory conditions.
Soil samples were incubated at 20 ± 2°C in darkness under aerobic conditions for 28 days. The soil moisture content was adjusted to 40% MWHC at weekly intervals by weight, using distilled-grade water, throughout the study.
For each test, 3 replicate soil samples were prepared at each treatment rate. At intervals of 0-3 h, 7, 14 and 28 days post-application samples of soil (ca 50 g oven dry equiv.) were removed from each replicate for analysis.
For the determination of soil respiration activity at each sample point single subsamples of soil from each soil replicate were removed for measurement of soil respiration.
For the determination of nitrate at each sample point, single subsamples of lucerne-amended soil were removed from each replicate for analysis. - Nominal and measured concentrations:
- Nominal: 150 g a.i./ha equivalent to 0.2 mg a.i./kg dws and 5 times this rate, 750 g a.i./ha equivalent to 1.0 mg a.i./kg dws
- Reference substance (positive control):
- yes
- Remarks:
- dinoseb acetate
- Key result
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- other: Nitrate concentrations
- Key result
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- respiration rate
- Details on results:
- Soil samples used to evaluate pesticide effects were supplemented with 5.0 g glucose per kg oven dry soil equiv. prior to measuring soil respiration.
At 0-3 h, 7, 14 and 28 days post-application, the CO2 evolution were followed over a 12 h period in the glucose amended subsamples.
Levels of nitrate in soil, which had not been amended with lucerne or treated with test material, increased from 5.2 mg to 23.3 mg N./kg over the incubation period, demonstrating significant nitrification activity. - Results with reference substance (positive control):
- At 0-3 h, 14 and 28 days post-application, with dinoseb acetate, the short-term substrate-induced respiration of treated soil deviated from untreated controls by greater than 25%, all of which were statistically significant.
- Validity criteria fulfilled:
- not specified
- Remarks:
- According to the current OECD guideline the validity criteria are fullfiled, please refer to “Any other information on results incl. tables”.
- Conclusions:
- The present guideline study was conducted in compliance with GLP. Under the test conditions used, the NOEC (28-day) was > 1.0 mg a.s./kg dws for nitrate content and respirtaion rate of soil microorganisms.
Reference
Please refer to "overall remark/ attached background material" field for result tables.
Table 1: Validity criteria for OECD 216 and OECD 217 (2000)
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
The variation of nitrate concentrations between replicate control samples should be less than ± 15%. |
max RSD nitrogen content: 0.06% max RSD carbon rate: 0.04% |
Yes |
Description of key information
The test material, applied at 0.2 and at 1 mg a.s./kg dry wt. soil, had no negative influence on nitrogen transformation carbon transformation in a sandy loam.
Key value for chemical safety assessment
- Long-term EC10 or NOEC for soil microorganisms:
- 1 mg/kg soil dw
Additional information
A key study investigating the toxicity of test material to N-cycle and C-cycle of soil microorganisms was available (1999). This test was conducted according to OECD guideline No. 216 and 217. The test material was incubated in the soil for 28 days at two concentration rates (150 and 750 g a.s./ha equivalent to 0.2 and 1 mg a.s./kg dws, respectively). The results found in the treatment groups regarding nitrogen concentrations and carbon transformation rates did not show any impact of this substance on soil microorganisms after 28 days.
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