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EC number: 433-460-1 | CAS number: 210880-92-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26 Jul - 21 Jul, 2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 1993
- Deviations:
- no
- Remarks:
- stated in the report. According to the current OECD guideline: fish length is higher than the recommended range of maximum 3 cm, environmental parameters not measured in each test vessel. These deviations have no impact on the outcome of the study.
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Water samples were collected from each replicate test chamber at approximately 0 and 96 hours to measure concentrations of the test substance. The samples were collected from mid-depth in each test chamber, placed in glass scintillation vials and analyzed immediately.
- Vehicle:
- no
- Details on test solutions:
- A primary stock solution was prepared by dissolving the test material in dilution water at a concentration of 120 mg a.i./L. The primary stock was mixed and sonicated overnight. The resultant solution appeared clear and colorless. 50 L aliquots of this test solution were distributed into each of three replicate test chambers for the definitive test.
- Test organisms (species):
- Lepomis macrochirus
- Details on test organisms:
- All fish used in the test were from the same source and year class. The bluegill were held for approximately 15 weeks prior to the test. The fish were acclimated to test conditions for approximately 54 hours prior to test initiation.
During the holding period, the bluegill were fed daily a commercially-prepared diet and live brine shrimp nauplii (Artemia sp.). The fish were not fed during the acclimation period (at least two days prior to the test) or during the test.
The test organisms were transferred to the test chambers within 30 minutes of preparation. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 128 mg/L
- Test temperature:
- 21.0 to 22.0°C
- pH:
- 7.8 to 8.5
- Dissolved oxygen:
- 7.6 to 8.4 mg/L
- Salinity:
- Not applicable
- Conductivity:
- 330 µmhos/cm
- Nominal and measured concentrations:
- Nominal: 201 mg a.i./L
Mean measured: 117 mg a.i./L - Details on test conditions:
- The water used for culturing and testing was freshwater obtained from a well approximately 40 m deep located on the Wildlife International, Ltd. site. The well water is characterized as moderately-hard water.
Test chambers were 19 L glass aquaria containing 15 L of test solution. The depth of water in a representative test chamber was approximately 18.2 cm. - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 117 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Details on results:
- Bluegill in the negative control group appeared normal and healthy throughout the test, with no mortalities or overt signs of toxicity observed. Similarly, all bluegill in the treatment group appeared normal and healthy throughout the test, with no mortalities or overt signs of toxicity observed.
- Sublethal observations / clinical signs:
Please refer to "overall remark/ attached background material" field for result tables.
Validity criteria for OECD 203 (2019)
Criterion from the guideline
Outcome
Validity criterion fulfilled
The mortality in the control(s) should not exceed 10% (or one fish if less than ten are used) at the end of the test
0%
Yes
The dissolved oxygen concentration must have been at least 60 per cent of the air saturation value throughout the test
Ranged between 7.6 to 8.4 mg/L
(5.2 mg/L = 60%)
Yes
There must be evidence that the concentration of the substance being tested has been satisfactorily maintained, and preferably it should be at least 80% of the nominal concentration throughout the test. If the deviation from the nominal concentration is greater than 20%, results should be based on the measured concentration.
The measured values were greater than 80 % of the nominal value in all aquaria over the whole test period
Yes
- Validity criteria fulfilled:
- not specified
- Remarks:
- in the report. Valid according to the current guideline, for further details please refer to “Any other information on results incl. tables”
- Conclusions:
- The present guideline study was conducted in compliance with GLP. Under the test conditions used, the LC50 96h for Lepomis macrochirus was > 117 mg a.s./L.
Reference
Description of key information
From short-term key study, the 96-hour LC50 for fish exposed to the present test material was > 117 mg a.s./L in freshwater.
From short-term supportive study, the 96-hour LC50 for fish exposed to the present test material was > 100 mg a.s./L for in saltwater.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- > 117 mg/L
Marine water fish
Marine water fish
- Dose descriptor:
- LC50
- Remarks:
- supportive study
- Effect concentration:
- > 100 mg/L
Additional information
Four GLP-studies are available for short-term toxicity testing in fish and one of them was identified as key study.
In the key study (2000), the acute toxicity of clothianidin to fish was investigated in a 96-hours static test with the bluegill sunfish (Lepomis macrochirus) according to guideline OECD 203. Ten fish per replicate and three replicate per concentration were exposed for 96 hours under static conditions to a limit nominal concentration of 120 mg as/L, alongside with a control. The 96-hour LC50was determined to be > 117 mg as/L. based on mean measured concentration values. Based on this study, the LC50for bluegill sunfish exposed to clothianidin in the freshwater was > 117 mg as/L.
There are three additional studies available, conducted with Oncorhynchus mykiss (rainbow trout),Cyprinodon variegatus (sheepshead minnow) and Lepomis macrochirus (bluegill); the resulting LC50values were > 100 mg a.s./L. These studies were considered suitable for supporting purpose.
In the first supporting study (2000), the acute toxicity of test material to rainbow trout was determined in a 96-h-static limit test according to guideline OECD 203.The 96-hour LC50of the technical active ingredient was determined to be > 100 mg as/L based on the nominal concentration.
In the second supporting study (1999), the acute toxicity of test material to sheepshead minnow was determined in a 96-h-static limit test according to OPPTS 850.1075 guideline.The 96-hour LC50of the technical active ingredient was determined to be > 100 mg a.s./L, based on the nominal concentration. Based on this study, the LC50for sheepshead minnow exposed to clothianidin in the saltwater was > 100 mg as/L.
In the third supporting study (2000), the acute toxicity of test material to bluegill sunfish was determined in a 96-h-static limit test according to OECD 203 guideline.The 96-hour LC50of the technical active ingredient was determined to be > 100 mg a.s./L, based on the nominal concentration.
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