Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 249-528-5 | CAS number: 29232-93-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to birds
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to birds: dietary toxicity test
- Remarks:
- reproduction
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- April 1975 to May 1975
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The studied laying hens were allowed a 28-day acclimatisation period prior to the start of egg production. This was followed by a 30-day period to ensure the birds had reached maximum egg production before the start of the 42-day experimental period. The birds were dietary exposed to the test substance for 28-day experimental period which followed by a 14-day recovery period. The number of eggs laid, quality and grade, fertility of eggs and their hatchability, chick (F1 generation) bodyweight and mortality up to 10 days of age and bodyweight and food consumption of parent birds were studied during this test.
- GLP compliance:
- no
- Dose method:
- feed
- Analytical monitoring:
- yes
- Remarks:
- See Methods used for determining residues in 'Any other information on materials and methdos incl. tables'
- Vehicle:
- yes
- Remarks:
- feed
- Details on preparation and analysis of diet:
- PREPARATION OF THE DIET
The commercial basic ration contain no additives. This ration was used for all birds with the required amount of test material added and the ration's composition was listed below:
- Wheat meal: 30%
- Maize meal: 20%
- Ex. soya meal: 20%
- Fish meal: 10%
- Maize starch: 10%
- Grass meal: 5%
- Limestone flour: 2.5%
- Coopers BTA40: 2.5%
The same ration was used for the chicks.
For the experimental diets a small premix was made after incorporating the required amount of the test substance in 0.5 kg of basal diet, This was mixed in a tumble mixer for 20 minutes, The premix was then added to the basal diet and incorporated mechanically. Fresh batches of experimental diet were prepared weekly. - Test organisms (species):
- other: Laying hen
- Details on test organisms:
- TEST ORGANISM
- Common name: Laying hen
- Source: Local breeder
- Age at arrival: 146 point-of lay (approximately 18 weeks old) pullets and sixteen 30 weeks old cockerels. On arrival the birds were examined to ensure there were no clinical signs of ill health, and they were allocated to pens- in treatment groups. - Limit test:
- no
- Total exposure duration (if not single dose):
- 42 d
- Remarks:
- 28-day experimental period on test diets with eggs collected for residue analysis and incubation and 14-day period with eggs collected for residue analysis only (all birds on control diet)
- Post exposure observation period:
- 14 days
- No. of animals per sex per dose and/or stage:
- - Group 1 (Basal diet only): 4 males + 37 females
- Group 2 (Basal diet + 4 mg test substance/kg diet): 4 males + 35 females
- Group 3 (Basal diet + 12 mg test substance/kg diet): 4 males + 37 females
- Group 4 (Basal diet + 40 mg test substance/kg diet): 4 males + 37 females - Control animals:
- yes, plain diet
- Nominal and measured doses / concentrations:
- - Nominal doses: 0 (negative control), 4, 12 and 40 mg test substance/kg diet.
- Measured doses: 4.1 – 5.2, 12.2 – 15.1 and 40.8 - 45.2 mg/kg diet, respectively. - Details on test conditions:
- ACCLIMATION
- Acclimation period: Four-week prior to the start of egg production. This was followed by a 30-day period to ensure that the birds had reached maximum egg production before the test stared.
PEN SIZE AND CONSTRUCTION MATERIALS (F0 generation)
- Description: The pens measured 2½ x 3 m and each pen housed one treatment group. Each pen contained an automatic drinker, food container, grit oontainer and nest boxes.
- Floor covering: The bedding used was wood shavings and these were replenished weekly,
NO. OF BIRDS PER SEX PER GROUP : See 'No.of animal per sex oer dose and/or stage'
NO. OF REPLICATES PER GROUP
- For vehicle control: 1
- For treated: 1
TEST CONDITIONS (F0 generation)
- Room temperature: The ambient temperature of thebuilding was 15 ˚C
- Photoperiod: 17 hours light and 7 hours darness
- Ventilation: Ventilation fans were adjusted to allow twelve air changes per hour.
- Feeding: The birds had access to food and water ad llbitum throughout the study.
EXPERIMENTAL PERCEDURE (F0 generation)
The cockerels were moved to a different treatment group after each 7-day experimental period. This was done to reduce possible effects of male infertility and meant that each group of 4 cockerels was placed with each group of hens for 7 days. This applied to the 28-day period of the study when eggs were collected for incubation. For the last 14-day period of the study when eggs were collected for residue analysis only, the cockerels were moved to the pens they occupied for the first 7-day period of the study.
All groups of birds were treated similarly, the only difference being in the diets offered.
TREATMENT OF CHICKS (F1 generation)
On hatching, the chicks remained in the incubator for approximately 12 hours before being transferred in treatment groups to a five-tiered brooder.
- Cage description: The birds were housed in groups in cages of metal construction measuring 80 x 36 x 24 cm.
- Room temperature: The ambient temperature of the brooder was maintained at 32 ˚C by means of thermostatically controlled heater units.
- Ventilation: The ventilation fans were adjusted to give eight air changes per hour.
- Feeding: The diet offered was similar to that offered to the parent generation and was given ad libitum. Water was available at all times. - Details on examinations and observations:
- MORTALITY / CLINICAL SIGNS
Recorded as they occurred.
BODY WEIGHT
The birds were weighed in groups on days 0, 7, 14, 21, 28 and 35 of the study.
FOOD CONSUMPTION
This was recorded over 7-day periods. The food consumption was that of the male and female birds combined.
BIRD HEALTH
All birds were examined daily for any toxic signs.
- Details on reproductive parameters:
- - Eggs incubation: All suitable eggs laid on days 1, 2, 4, 6, 8, 9, 11, 13, 15, 16, 18, 20, 22, 23, 25 and 27 of the study were incubated. After collection, selected eggs were stored at room temperature for 7 days and then placed in a incubator for 21 days at a temperature of 37 ˚C. On day 19 all eggs were moved into hatching trays in the bottom compartment of the incubator.
- Egg Production: This was recorded dally for the acclimatisation and test periods.
- Eggs quality: Each egg was examined for cracks and abnormal shape. Each egg was also graded using a modification of the weights specified by the 'Egg Marketing Board'. The eggs were weighed and allocated by weight to one of the following grades:
> Small: 0 - 42 g
> Medoum: 42 - 63 g
> Large: 64 - 112 g
- Candling: All eggs set in the incubator were candled for signs of fertility on days 7 and 18 of the incubation period,
- Hatching: The following results were recorded: 1) Number hatched alive; 2) Number dead in shell; 3) Number infertile; 4) Number broken during incubation and 5) Any abnormalities
- Chick rearing: All birds hatched were reared to 10 days of age and the following results were recorded: 1) Weight of chicks within 24 hours of hatching (day 0) and again on day 10 approximately, from which the bodyweight gain was obtained; 2) Mortalities and 3) Any abnormalities - Reference substance (positive control):
- no
- Key result
- Duration (if not single dose):
- 28 d
- Dose descriptor:
- NOEL
- Effect level:
- 40 mg/kg diet
- Conc. / dose based on:
- test mat.
- Basis for effect:
- reproductive parameters
- Mortality and sub-lethal effects:
- An overview of the results is provided in Table 2 - Table 3 in 'Any other information on results incl. tables'.
- Food consuption (F0 generation): The food consumption of the four treatment groups varied over the 7-day periods of the study. The results covering the 28-day inclusion period suggested that diets containing the test substance might be less palatable than the control diet. As only total food consumption figures for each group of birds over weekly periods were available for analysis and as time is a systematic factor and not a random factor, and also as the observations taken over different time periods on the same birds are not independent, it is not possible to do a strictly valid statistical analysis of the data. However, both non-parametric and classical two way analyses of variance were carried out on the data. No significant differences were obtained. Food consumption in all the groups was similar during the 2-week post-inclusion period when all groups were offered basal diet only.
- Bodyweight (F0 generation): The mean bodyweights of all groups increased over the test period. All bodyweight changes were considered to be within normal limits and the results indicate that no adverse effects on bodyweight resulted from the inclusion of the test substance in the diet of laying hens at concentrations up to 40 mg/kg diet.
- Mortality: One bird in the control group died on day 35 of the study. No other mortalities were recorded.
- Bird health: All surviving birds appeared to remain in good health and no signs of stress or abnormal behaviour were observed. - Effects on reproduction:
- An overview of the results is provided in Table 4 - Table 8 in 'Any other information on results incl. tables'.
- Egg production: All groups of birds offered diet containing the test substance laid more eggs than the control group. Results were analysed statistically by the two-way analysis of variance, the factors in the analysis being treatment groups and day of collection. Treatment with 4 mg/kg diet, 12 mg/kg diet and 40 mg/kg diet produced significantly more eggs than the control group (P < 0.001, P < 0.001 and P < 0.001 respectively).
- Egg quality: There was no marked difference between treatments in the number of cracked or broken eggs recorded. The results were confirmed statistically using non-parametric methods. From these results it appears that the inclusion of the test substance in the diet of laying hens had no adverse effects on egg quality. Results were confirmed statistically using Friedman's two-way analysis of variance.
(a) % of eggs laid that were grade S (small). There was no significant trend associated with dose.
(b) % of eggs laid that were grade M (medium). There was no significant trend associated with dose.
(c) % of eggs laid that were grade L (large). Group treated with 12 mg/kg diet was statistically different from the control group (P < 0.05) producing fewer grade L eggs. This result complements the fact that group treated with 12 mg/kg diet produced more grade M eggs than the control group.
- Fertility and hatchabillty:
(a) % of eggs incubated that were infertile: statistical analysis of the results indicate that there was no statistical difference between treatments.
(b) % of eggs incubated in which there were embryonic mortalities: statistical analysis of the results indicate that there was no statistical difference between treatments.
(c) % of eggs incubated which were hatched alive: statistical analysis of the results indicate that there was no statistical difference between treatments.
The fertility and hatchability results indicate that the inclusion of the test substance in the diet of laying hens had no adverse effects on the parameters measured.
- Chick weights (F1 generation): The results were analysed by non-parametric methods. No significant difference between treatments was observed. The group mean bodyweight increases of the four groups were as follows:
(a) Control: 37.1 g
(b) 4 mg/kg diet test substance treated group: 36.3 g
(c) 12 mg/kg diet test substance treated group: 37.2 g
(d) 40 mg/kg diet test substance treated group: 37.2 g
- Chick mortalities (F1 generation): The % mortalities in the four groups were as follows:
(a) Control: 11.2
(b) 4 mg/kg diet test substance treated group: 10.8
(c) 12 mg/kg diet test substance treated group: 11.1
(d) 40 mg/kg diet test substance treated group: 12.3
The mortalities of the four groups were considered to be within normal limits. - Validity criteria fulfilled:
- not specified
- Conclusions:
- Based on the findings, the NOEL was determined to be 40 mg/kg diet.
- Executive summary:
The effects of the test substance to birds (laying hen) was investigated in 28-day exposure study. The study did not follow any internationally established guidelines and was not in compliance with GLP criteria. However, the study was well documented and meets generally accepted scientific principles. The study was therefore considered to have a Klimisch 2 reliability score and was acceptable for assessment. 146 hens, purchased at 18 weeks of age, and 16 30-week-old cockerels were used in this study. The birds were allowed a 28-day acclimation period prior to the start of egg production. This was followed by a 30-day period to ensure the birds had reached maximum egg production before the start of the 28-day treatment plus 14-day recovery experimental period. The nominal doses were 0 (control, on plain diet only), 4, 12 and 40 mg/kg diet. The diet samples were analytically verified by gas chromatography and the mean measured doses were determined to be 4.1 – 5.2, 12.2 – 15.1 and 40.8 - 45.2 mg/kg diet, respectively. The measured test substance levels in egg white, liver and mixed flesh were below the levels of detection. For biological effect, the number of eggs laid, quality and grade, fertility of eggs and their hatchability, chick (F1 generation) bodyweight and mortality up to 10 days of age and bodyweight and food consumption of parent birds were studied in this test.
F0 generation: Birds offered diet containing the test substance laid more eggs than the control group. But there was no statistical difference between treatments. The number of cracked and broken eggs was recorded. All other eggs were graded according to weight. The results indicate that the dietary inclusion of the test substance had no adverse effects on egg quality and egg weight. The number of large eggs produced by 12 mg/kg diet treated group was lower than the other groups (significant at the P < 0.05 level). None of the other results were statistically significant. The three groups offered diet containing the test substance consumed less food than the control group over the 28-day inclusion period, although this difference was not statistically significant. The food consumptions of the three test groups and the control group were similar over the two-week post-inclusion period. The mean bodyweights of all groups increased over the test period. All bodyweight changes were considered to be within normal limits. The results indicate that the inclusion of the test substance in the diet at various levels had no adverse effects on bodyweight. One bird in the control group died on day 35 of the study. No other mortalities were recorded. All surviving birds appeared to remain in good health and no signs of stress or abnormal behaviour were observed. The results of all groups were considered to be within normal limits. Statistical evaluation of the results revealed no significant difference between treatments. No adverse effects were observed that could be related to treatment.
F1 generation: All results of chick mortality were considered to be within normal limits and there was no significant difference between treatments. There was no significant difference of chick bodyweights between treatments. Overall, all results were considered to be within normal limits and there was no significant difference between treatments. Therefore, the NOEL was determined to be 40 mg/kg diet.
Reference
Analytical data
- Egg yolk: There were indications that the test substance concentrations in egg yolks in 40 mg/kg diet reached a plateau level by day 7, as the concentrations on days 7, 14 and 21 were similar. The day 28 concentration was only slightly higher. Following the 7-day withdrawal periods, values were below the level of detection. The results for the other three groups following analysis for the test substance were on or below the level of detection. The results for all groups following analysis for the metabolites of the test substance were below the levels of detection.
- Egg white, liver and flesh: The measured test substance levels in egg white, liver and mixed flesh were below the levels of detection.
Table 2. Group mean food consumption (F0 generation)
Group |
Inclusion level (mg test substance/kg diet) |
Food consumption (kg/bird) |
||||||||
Period of study |
Total |
|||||||||
0 - 7 days |
8 - 14 days |
15 - 21 days |
22 - 28 days |
29 - 35 days |
36 - 42 days |
0 - 28 days |
29 - 42 days |
0 - 42 days |
||
1 |
0 |
1.14 |
1.03 |
1.03 |
0.98 |
1.15 |
0.83 |
4.18 |
1.98 |
6.16 |
2 |
4 |
0.89 |
0.97 |
1.03 |
0.95 |
1.05 |
0.77 |
3.84 |
1.82 |
5.66 |
3 |
12 |
0.99 |
1.26 |
0.83 |
0.95 |
1.12 |
0.86 |
4.03 |
1.98 |
6.01 |
4 |
40 |
0.95 |
0.96 |
1.10 |
0.82 |
1.13 |
0.79 |
3.83 |
1.92 |
5.75 |
Table 3. Group mean body weight (F0 generation, females only)
Group |
Inclusion level (mg test substance/kg diet) |
0 |
7 |
14 |
21 |
28 |
35 |
Increased (g/bird) |
1 |
0 |
1925 |
1930 |
1989 |
2019 |
1996 |
1930 |
5 |
2 |
4 |
1957 |
1911 |
1940 |
1969 |
1984 |
1975 |
18 |
3 |
12 |
1920 |
1971 |
1969 |
1961 |
1967 |
1977 |
57 |
4 |
40 |
1933 |
1926 |
1928 |
1950 |
1938 |
1999 |
66 |
Table 4. Summary of reproduction parameters
Group |
Treatment level (mg/kg diet) |
Egg quality as % of egg laid |
Total eggs laid |
Total egg incubated |
F1 generation |
|||||||||
Hatched alive % |
Embryonic mortalities % |
Infertile % |
Body weights (g) |
Mortalities % of eggs incubated |
||||||||||
Rejected |
small |
Medium |
Large |
0 days |
10 days |
Increased 1 - 10 days |
||||||||
1 |
0 |
6.8 |
3.6 |
80.5* |
7.4 |
365 |
182 |
63.5 |
22.1 |
14.3 |
41.3 |
78.4 |
37.1 |
11.2 |
2 |
4 |
6.3 |
2 |
84.4* |
5.9 |
507 |
270 |
66.3 |
20.8 |
13.0 |
41 |
77.3 |
36.3 |
10.8 |
3 |
12 |
3.7 |
4.8 |
89 |
2.5 |
481 |
278 |
61.9 |
23.4 |
14.7 |
40.5 |
77.7 |
37.2 |
11.1 |
4 |
40 |
6 |
3.4 |
83.8 |
6.8 |
498 |
268 |
68.3 |
21.3 |
10.4 |
40.6 |
77.8 |
37.2 |
12.3 |
Table 5. Number of eggs laid/group (0-28 days)
Group |
Inclusion level (mg test substance/kg diet) |
Eggs laid |
Total eggs laid |
|||
Week 1 |
Week 2 |
Week 3 |
Week 4 |
|||
1 |
0 |
96 |
77 |
114 |
78 |
365 |
2 |
4 |
101 |
119 |
154 |
133 |
507 |
3 |
12 |
136 |
123 |
121 |
101 |
481 |
4 |
40 |
124 |
121 |
150 |
103 |
498 |
Table 6. Number of cracked or broken eggs
Group |
Inclusion level (mg test substance/kg diet) |
Total eggs laid (days 0 -28) |
Eggs broken |
Eggs cracked |
||
actual |
% |
actual |
% |
|||
1 |
0 |
365 |
6 |
1.6 |
19 |
5.2 |
2 |
4 |
507 |
11 |
2.2 |
21 |
4.1 |
3 |
12 |
481 |
6 |
1.2 |
12 |
2.5 |
4 |
40 |
498 |
8 |
1.6 |
22 |
4.4 |
Table 7. Number of eggs in each weight range
Group |
Inclusion level (mg test substance/kg diet) |
Total eggs laid (days 0 -28) |
Egg rejected |
Small |
Medium |
Large |
||||
actual |
% |
actual |
% |
actual |
% |
actual |
% |
|||
1 |
0 |
365* |
25 |
6.8 |
13 |
3. 6 |
294 |
80.5 |
27 |
7.4 |
2 |
4 |
507* |
32 |
6.3 |
10 |
2 |
428 |
84.4 |
30 |
5.9 |
3 |
12 |
481 |
18 |
3. 7 |
23 |
4.8 |
428 |
89.0 |
12 |
2.5 |
4 |
40 |
498 |
30 |
6.0 |
17 |
3.4 |
417 |
83.8 |
34 |
6.8 |
* Some eggs were broken after collection and prior to grading (Group 1: 6 eggs, Group 2: 7 eggs).
Table 8. Summary of hatchability results (actual and %)
Group |
Hatched alive |
Embryonic mortalities |
Infertile |
|
||||||||||
Inclusion level (mg test substance/kg diet) |
Egg incubated |
actual |
% |
Early |
Fully formed |
Total |
actual |
% |
|
|||||
actual |
% |
actual |
% |
actual |
% |
|
||||||||
1 |
0 |
181* |
115 |
63.5 |
24 |
13.3 |
16 |
8.8 |
40 |
22.1 |
26 |
14.3 |
||
2 |
4 |
270 |
179 |
66.3 |
34 |
12.6 |
22 |
8.1 |
46 |
20.7 |
35 |
13 |
||
3 |
12 |
278 |
172 |
61.9 |
30 |
10.8 |
35 |
12.6 |
65 |
23.4 |
41 |
14.7 |
||
4 |
40 |
268 |
183 |
68.3 |
34 |
12.7 |
23 |
8.6 |
57 |
21.3 |
28 |
10.4 |
* Results from day 15 not included in calculation of means as only one egg was incubated.
Description of key information
All available data was assessed and the study representing the worst-case effects was included here as key studies. These results are selected for the CSA.
28-d NOEL = 40 mg/kg diet, laying hen, reproduction parameters, no guideline followed, Ross 1978
Key value for chemical safety assessment
- Long-term EC10, LC10 or NOEC for birds:
- 40 mg/kg food
Additional information
The effects of the test substance to birds (laying hen) were investigated in a 28-day exposure study. The study did not follow any internationally established guidelines and was not in compliance with GLP criteria. However, the study was well documented and meets generally accepted scientific principles. The study was therefore considered to have a Klimisch 2 reliability score and was acceptable for assessment. 146 hens, purchased at 18 weeks of age, and 16 30-week-old cockerels were used in this study. The birds were allowed a 28-day acclimation period prior to the start of egg production. This was followed by a 30-day period to ensure the birds had reached maximum egg production before the start of the 28-day treatment plus 14-day recovery experimental period. The nominal doses were 0 (control, on plain diet only), 4, 12 and 40 mg/kg diet. The diet samples were analytically verified by gas chromatography and the mean measured doses were determined to be 4.1 – 5.2, 12.2 – 15.1 and 40.8 - 45.2 mg/kg diet, respectively. The measured test substance levels in egg white, liver and mixed flesh were below the levels of detection. For biological effect, the number of eggs laid, quality and grade, fertility of eggs and their hatchability, chick (F1 generation) bodyweight and mortality up to 10 days of age and bodyweight and food consumption of parent birds were studied in this test.
F0 generation: Birds offered diet containing the test substance laid more eggs than the control group. But there was no statistical difference between treatments. The number of cracked and broken eggs was recorded. All other eggs were graded according to weight. The results indicate that the dietary inclusion of the test substance had no adverse effects on egg quality and egg weight. The number of large eggs produced by 12 mg/kg diet treated group was lower than the other groups (significant at the P < 0.05 level). None of the other results were statistically significant. The three groups offered diet containing the test substance consumed less food than the control group over the 28-day inclusion period, although this difference was not statistically significant. The food consumptions of the three test groups and the control group were similar over the two-week post-inclusion period. The mean bodyweights of all groups increased over the test period. All bodyweight changes were considered to be within normal limits. The results indicate that the inclusion of the test substance in the diet at various levels had no adverse effects on bodyweight. One bird in the control group died on day 35 of the study. No other mortalities were recorded. All surviving birds appeared to remain in good health and no signs of stress or abnormal behaviour were observed. The results of all groups were considered to be within normal limits. Statistical evaluation of the results revealed no significant difference between treatments. No adverse effects were observed that could be related to treatment.
F1 generation: All results of chick mortality were considered to be within normal limits and there was no significant difference between treatments. There was no significant difference of chick bodyweights between treatments. Overall, all results were considered to be within normal limits and there was no significant difference between treatments. Therefore, the NOEL was determined to be 40 mg/kg diet.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.