Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 601-147-9 | CAS number: 111988-49-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 21 Sep - 13 Oct 1994
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- Version / remarks:
- adopted 2009
- Deviations:
- yes
- Remarks:
- MMAD and GSD slightly out of range
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- Version / remarks:
- adopted 1981
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- traditional method
- Limit test:
- no
Test material
- Reference substance name:
- 3-(2-chlor-5-pyridyl-methyl)-cyanimino-1,3-thiazolidin
- EC Number:
- 601-147-9
- Cas Number:
- 111988-49-9
- Molecular formula:
- C10H9ClN4S
- IUPAC Name:
- 3-(2-chlor-5-pyridyl-methyl)-cyanimino-1,3-thiazolidin
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Harlan-Winkelmann, Borchen, Germany
- Females nulliparous and non-pregnant: yes
- Rationale for use of males: male and female rats were used in this study
- Age at study initiation: 2 - 3 months
- Weight at study initiation: 160.0 - 210.0 g (males), 162.0 - 191.0 g (females)
- Housing: individually in Makrolon Type II cages equipped with type S8/15 low dust wood granulate (Ssniff, Soest, Germany) as bedding material
- Diet: Altromin 1324 pellets maintenance diet for rats and mice (Altromin GmbH, Lage, Germany), ad libitum
- Water: tap water, ad libitum
- Acclimation period: at least 5 days
- Method of randomisation in assigning animals to test and control groups: computerized list of random numbers
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 - 24
- Humidity (%): approx. 50
- Air changes (per hr): approx. 10
- Photoperiod (hrs dark / hrs light): 12 / 12
Administration / exposure
- Route of administration:
- inhalation: dust
- Type of inhalation exposure:
- nose only
- Vehicle:
- clean air
- Mass median aerodynamic diameter (MMAD):
- >= 3.1 - <= 9.1 µm
- Geometric standard deviation (GSD):
- >= 1.7 - <= 3.5
- Remark on MMAD/GSD:
- MMAD (Mass median aerodynamic diameter) / GSD (Geometric st. dev.): 3.1 µm / 1.7 µm (0.080 mg/L), 3.1 µm / 1.7 µm (0.481 mg/L), 5.8 µm / 3.5 µm (1.523 mg/L), 9.1 µm / 2.4 µm (2.535 mg/L)
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: Plexiglas exposure tubes
- Exposure chamber volume: about 3.8 L
- Method of holding animals in test chamber: plexiglas exposure tubes that were chosen to fit the size of the animals
- Source and rate of air (airflow): 28 L/min
- Method of conditioning air: via compressed air dryer, i.e. water, dust and oil were removed
- System of generating particulates/aerosols: EXACTOMAT 4200 (TSE, Bad Homburg, Germany) and for the lower concentrations (0.080 and 0.481 mg/L) a Wright-Dust-Feeder (BGI Inc., Waltham, MA, USA)
- Method of particle size determination: Cascade impactors
- Treatment of exhaust air: purified via cotton-wool/activated charcoal and HEPA filters
- Temperature, humidity, pressure in air chamber: 23 - 24 °C, 10 - 38%, not specified
TEST ATMOSPHERE
- Brief description of analytical method and equipment used: test-substance concentration was determined by gravimetric analysis using cellulose-acetate filters (Sartorius, Göttingen, Germany)
- Samples taken from breathing zone: yes, once per hour
- Time needed for equilibrium of exposure concentration before animal exposure: < 1 min
VEHICLE
- Composition of vehicle: conditioned air
TEST ATMOSPHERE
- Particle size distribution: number of particles < 3 µm: 49% (0.080 mg/L), 48% (0.481 mg/L), 31% (1.523 mg/L), 10% (2.535 mg/L)
- MMAD (Mass median aerodynamic diameter) / GSD (Geometric st. dev.): 3.1 µm / 1.7 µm (0.080 mg/L), 3.1 µm / 1.7 µm (0.481 mg/L), 5.8 µm / 3.5 µm (1.523 mg/L), 9.1 µm / 2.4 µm (2.535 mg/L) - Analytical verification of test atmosphere concentrations:
- yes
- Duration of exposure:
- 4 h
- Concentrations:
- 0.080, 0.481, 1.523 and 2.535 mg/L
- No. of animals per sex per dose:
- 5
- Control animals:
- yes
- Remarks:
- Control studies are performed regularly under GLP conditions, but not assigned to a specific study.
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: body weights were measured before exposure, on days 3, 7 and weekly thereafter. Individual weights were also recorded at death, if applicable. Appearance and behavior were examined several times on the day of exposure and twice daily thereafter (once on weekends)
- Necropsy of survivors performed: yes
- Other examinations performed: rectal temperature, reflex measurements - Statistics:
- Please refer to 'any other information on materials and methods incl. tables' section for details.
Results and discussion
Effect levelsopen allclose all
- Key result
- Sex:
- female
- Dose descriptor:
- LC50
- Effect level:
- ca. 1.223 mg/L air (analytical)
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Key result
- Sex:
- male
- Dose descriptor:
- LC50
- Effect level:
- > 2.535 mg/L air (analytical)
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Mortality:
- Mortality was not observed in males at any dose level and not in females of the control, 0.080 and 0.481 mg/L dose groups. At 1.523 mg/L 3/5 females died 1 to 2 days after exposure and at 2.535 mg/L 4/5 females died 1 to 7 days after exposure.
- Clinical signs:
- other: Please refer to 'any other information on results incl. tables' section for details.
- Body weight:
- Males and females of the 0.080 mg/L dose group tolerated the exposure without marked effects on body weights when compared to controls. However, at levels of 0.481 mg/L of test item and higher doses, rats lost body weight during the first three days after exposure (2.535 mg/L dosed females lost body weight until one week after exposure). Thereafter, all rats gained body weight until the close of the study, but body weight gain was overall reduced at dose levels of 0.481 mg/L and higher when compared to controls.
- Gross pathology:
- In rats sacrificed at the end of the observation period (survivors), no compound-related increase in the incidence of macroscopical findings could be observed. In rats sacrificed during the observation period, lungs with reddish colour and red foci, intestine with red mucosa, reddish-slimy content, pale and lobulated livers and red appearance of renal pelvis was observed.
- Other findings:
- All animals showed normal reflexes, except for some alteration in reflexes in the 1.523 mg/L dose group (males and females). Rectal temperature was concentration-dependently decreased in animals exposed to the test item when compared to controls. Changes observed in the 0.080 mg/L dose group were considered toxicologically not relevant.
Any other information on results incl. tables
Clinical signs
No clinical signs were observed in the control and 0.080 mg/L dose group. At 0.481 mg/mL, bradypnoea, tremor, reduced motility, haircoat ungroomed and piloerection were observed starting from 4 h and lasting until 2 days after exposure. In the 1.523 mg/mL groups, bradypnoea, laboured breathing pattern, rales, prostration (lying on side or belly), blepharospasm, mydriasis, chromodacryorrhea, tremor, reduced motility, apathy, haircoar ungroomed and piloerection were noted from 4 h and lasting until 4 (females) or 6 (males) days after exposure. In the top dose group (2.535 mg/mL), bradypnoea, dyspnoea, laboured breathing pattern, rales, nose/snout area with red encrustation, salivation, blepharospasm, mydriasis, tremor, reduced motility, haircoat ungroomed and piloerection were observed from 4 h until 4 (males) or 6 (females) days after study.
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- The study was performed in accordance to OECD TG 403 under GLP conditions and is considered reliable. Under the conditions chosen, the acute inhalation LC50 was above 2.535 mg/L for male Wistar rats and approximately 1.223 mg/L for female Wistar rats. According to criteria of the CLP Regulation (EU) No. 1272/2008, classification of the test item for acute inhalation toxicity category 4 (H332) is needed.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.