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EC number: 500-046-6 | CAS number: 26183-52-8 1 - 2.5 moles ethoxylated
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Justification for type of information:
- Please refer to the Category Approach Justification provided in Section 13
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 1.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 20 - 24 JAN 1997 and 03 - 07 FEB 1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Trade Name: Marlowet BL
- Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- TEST ORGANISM
- Common name: common carp (Cyprinus carpio)
- Source: Bio International B.V., Someren, NL
- Length at study initiation (length definition, mean, range and SD): 3 - 4 cm
- Feeding during test: no feeding
ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions (same as test or not): 200 L dechlorinated drinking water in 300 L glass aquaria at 20°C ± 2°C, flow-through system
- Type and amount of food: Truovit, 1% of body weight
- Feeding frequency: daily
- Health during acclimation (any mortality observed): 7 days prior test start: <= 5% - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 1st test: 12.5 ° dH
2nd test: 12 ° dH - Test temperature:
- 1st test: mean: 20 °C (19.4 - 20.7 °C)
2nd test: mean: 21 °C (20.5 - 20.9 °C) - pH:
- 1st test: 8.0 - 8.2
2nd test: 8.0 - 8.3 - Dissolved oxygen:
- 1st test: 93% - 97%
2nd test: 72% - 97% - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 20 L aquaria filled with 10 L test medium
- Renewal rate of test solution (frequency/flow rate): daily
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.67 g
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: drinking water Gelsenwasser AG
OTHER TEST CONDITIONS
- Photoperiod: 16 light / 8 h darkness - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 1.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 1.0 - 1.7
- Sublethal observations / clinical signs:
Concentration
[mg/L]Test period (h)
24 h
48 h
72 h
96 h
Mortality [%]
Mortality [%]
Mortality [%]
Mortality [%]
1st test
Control
0
0
0
0
1.0
0
0
20
20
1.7
10
100
100
100
3.0
100
100
100
100
5.0
100
100
100
100
9.0
100
100
100
100
2nd test
Control
0
0
0
0
0.3
0
0
0
0
0.6
0
0
0
0
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 07 - 11 NOV 1994
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Trade name: Marlipal 24/20
- Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 1 g of test substance/L was diluted in demineralized water and stirred over 18 h and filtered subsequently - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: zebrafish
- Source: West Aquarium, Bad Lauterberg, Germany
- Length at study initiation (length definition, mean, range and SD): 3 cm +/- 0.5 cm
- Feeding during test: no feeding
ACCLIMATION
- Acclimation period: 14 days quarantine after treatment with malachit-green
- Acclimation conditions (same as test or not): dechlorinated drinking water in 200 L stone ware aquaria or 300 L glas aquaria, 20 °C +/- 2 °C, flow-
through conditions
- Type and amount of food: TetraMin approx. 1% of body weight
- Feeding frequency: daily
- Health during acclimation (any mortality observed): animals were healthy at test start, mortality <= 5% prior test start - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 10.8 ° dH (2nd test), 10.5 ° dH (3rd test)
- Test temperature:
- 20 °C
- pH:
- 7.8 - 8.3 (2nd test)
7.8 - 8.4 (3rd test) - Dissolved oxygen:
- 80 - 101% (2nd test)
98 - 106% (3rd test) - Nominal and measured concentrations:
- nominal: 0.12, 0.25, 0.50, 1.0, 2.0 mg/L (2nd test) and 3.6 and 6.0 mg /L (3rd test)
mean measured (geometric mean): 0.12, 0.12, 0.13, 0.46, 0.66 mg/L (2nd test) and 2.4 and 4.4 mg/L (3rd test) - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 20 L test vessels filled with 10 L test water
- Aeration: continuous
- Renewal rate of test solution (frequency/flow rate): semi-static test - daily renewal of test water
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.25 g/fish (2. test), 0.20 g/fish (3. test)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: drinking water (Gelsenwasser AG)
- Intervals of water quality measurement: daily
OTHER TEST CONDITIONS
- Photoperiod: 16 light / 8 h darkness - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 1.2 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.66 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Sublethal observations / clinical signs:
Mean measured concentration
[mg/L]Test period (h)
24 h
48 h
72 h
96 h
Mortality [%]
Mortality [%]
Mortality [%]
Mortality [%]
2nd test
Control
0
0
0
0
0.12
0
0
0
0
0.12
0
0
0
0
0.13
0
0
0
0
0.46
0
0
0
0
0.56
0
0
0
0
3rd test
Control
0
0
0
0
2.4
100
100
100
100
4.4
100
100
100
100
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 96-hour LC50 of Alcohols, C12-14, ethoxylated (2 EO) to Brachydanio rerio was investigated in accordance to EU Guideline 92/69 EWG Part C 1. The LC50 was determined to be 1.2 mg/l.
Referenceopen allclose all
Description of key information
LC50 (96h) = 1.2 mg/L for fish (Cyprinus carpio and Danio rerio)
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 1.2 mg/L
Additional information
Since no data for decan-1-ol, ethoxylated, 1-2.5EO (CAS 26183-52-8), are available the acute toxicity on fish is assessed on the basis of data from studies with similar alcohol ethoxylates (C12, 4 EO; CAS 9002-92-0, and C12-14, 2EO; CAS 68439-50-9). As aquatic toxicity increases with an increase in the length of the hydrocarbon chain the resulting LC50 values can be considered as worst case.
The first study was carried out according to GLP and EU Method C.1 (Acute Toxicity for Fish) with analytical verification of the test concentrations (Sasol, 1997). Carp (Cyprinus carpio) was exposed for 96 h under semi-static conditions. Based on nominal concentrations, an LC50 (96h) of 1.2 mg/L is reported.
The second study (Sasol, 1995) was carried out according to GLP and EU Method C.1 (Acute Toxicity for Fish) as well. And analytical verification of the test concentrations was performed. Zebrafish (Danio rerio) was exposed for 96 h under semi-static conditions. Based on measured concentrations (geo. mean), an LC50 (96h) of 1.2 mg/L was derived.
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