Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
Phenol, paraalkylation products with C10-15 branched olefins (C12 rich) derived from propene oligomerization, carbonates, calcium salts, overbased, sulfurized, including distillates (petroleum), hydrotreated, solvent-refined, solvent-dewaxed, or catalytic dewaxed, light or heavy paraffinic C15-C50
EC number: 701-251-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- 2nd April to 4th May 1998
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study following GLP. Reliability rating reduced to 2 as study read across from Phenol, tetrapropenyl-, sulfurized, calcium salts CAS No. 68855-45-8.
- Justification for type of information:
- See attached category justification in section 13
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- not specified
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure):
Activated sludge was collected from the Prospect Bay Wastewater Treatment Facility on April 01, 1998. The sludge was sieved using a 2 mm screen and then aerated for approximately four hours. The sludge was homogenized in a blender at medium speed for approximately 2 minutes and then was allowed to settle for approximately 30 minutes. The supernatant was used as the inoculum for this study and was used the same day that it was prepared. A total suspended solids measurement and standard plate count were performed on the inoculum using procedures based on Standard Methods. The plates were incubated at 20 ± 3 °C for approximately 48 hours and then the number of colony forming units was enumerated. - Duration of test (contact time):
- 28 d
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
The test medium was a modified biochemical oxygen demand (BOD) test dilution water and was prepared using high quality water as described in the Protocol.
- Test temperature: 20 ± 3ºC.
- pH: The pH values ranged from 5.2 to 6.5.
- Aeration of dilution water: The sludge was sieved using a 2 mm screen and then aerated for approximately four hours.
- Suspended solids concentration: The average measured total suspended solids (TSS) concentration of the inoculum was 78.6 mg/L. The solids concentration of the test solution was 0.786 mg/L and was within the acceptable limit specified in the test guideline.
Concentration of test chemical: An amount of test substance necessary to deliver 10 mg Carbon/L was added to the treatment group by direct
weight addition.
TEST SYSTEM
- Culturing apparatus:
The test chambers were ambered 4-liter bottles. The air entering the chambers was passed through Drierite to remove ambient moisture and then through Ascarite to produce CO2 free air. The air exiting the test chambers was passed through a series of three gas washing bottles each containing approximately 100 mL of 0.5 N KOH to trap the CO2 that had evolved within the chamber. An additional set of gas washing bottles that were not connected to a chamber were maintained concurrently with the traps connected to the chambers. These bottles contained approximately 100 mL of 0.5 N KOH and the amount of CO2 detected in the KOH solution was subtracted from the CO2 in the blank control traps to determine the amount of CO2 produced by the inoculum in the blank control. The test was conducted at 20 ± 3 °C. Test chambers were identified by project number, test substance ID, test concentration, and vessel number. Magnetic stir bars and stir plates were used to mix the contents of the test chambers. Stir plate motors were cycled on and off approximately every 15 minutes to prevent the heating of the stirrer motors.
SAMPLING
- Sampling frequency:
The CO2 traps were removed for analysis on days 1, 4, 8, 11, 14, 19, 21, 25, and 29. The CO2 trap nearest the chamber was removed and analyzed for inorganic carbon. The two remaining traps were placed one position closer to the test chamber and a new trap was placed on the end of the series.
- Sampling method:
On the 28th day of the test, an aliquot of the contents of each test chamber was removed and the pH determined. The contents of each chamber then were acidified by the addition of 3 mL of concentrated hydrochloric acid to drive off inorganic carbonate. The chambers were aerated overnight after which the trapping solutions closest to the test chambers were analyzed for inorganic carbon.
- Sample storage before analysis: The test substance was stored under ambient conditions.
CONTROL AND BLANK SYSTEM
- Inoculum blank: The blank control was used to measure the background CO2 production of the inoculum and was not dosed with a carbon source.
- Toxicity control: The reference chambers were used to check the viability of the inoculum and were dosed with sodium benzoate, a substance known to be biodegradable, at a concentration of 10 mg Carbon/L. - Reference substance:
- benzoic acid, sodium salt
- Test performance:
- The viability of the inoculum and validity of the test were supported by the results of the reference substance, sodium benzoate, from which 99.3% of theoretical CO2 was evolved. An average percent biodegradation of greater than 60% was achieved within 7 days, thereby fulfilling the criteria for a valid test by reaching the pass level by day 14
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 13.4
- Sampling time:
- 28 d
- Details on results:
- The average cumulative percent of theoretical carbon dioxide produced by the test substance at 10 mg C/L was 13.4%.
- Results with reference substance:
- Reference (sodium benzoate) – 99.3% (28d). An average percent biodegradation of >60% was achieved within 7 days, thereby fulfilling the criteria for a valid test reaching the pass level by day 14.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: Not readily biodegradable
- Conclusions:
- The test substance may not be considered readily biodegradable under the conditions tested since the pass level of 60% TCO2 was not achieved. However since tests of ready biodegradability provide limited opportunity for biodegradation to occur, a negative result does not necessarily mean that the test substance will not be biodegraded under relevant environmental conditions.
- Executive summary:
In a study on the ready biodegradability of the test substance in a CO2 Evolution Test (WILDLIFE INTERNATIONAL LTD. PROJECT NO.: 331E-114) conducted to OECD guideline 301 B (Ready Biodegradability: CO2Evolution Test) under conditions of GLP the test substance can be described as not readily biodegradable under the conditions of the test and only 13.4% degradation was seen in 28 days.
Evidence of ready biodegradability in a Carbon Dioxide Evolution Test is 60% TCO2 within the 28-day test period. In addition, the pass value must be reached within 10 days of achieving 10% TCO2. The test substance may not be considered readily biodegradable under the conditions tested since the pass level of 60% TCO2 was not achieved. However since tests of ready biodegradability provide limited opportunity for biodegradation to occur, a negative result does not necessarily mean that the test substance will not be biodegraded under relevant environmental conditions.
There was no available data to fulfil this endpoint for the test material and so the study was read-across from a supporting substance (EC 701-249-4).
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- 16th September to 17th October 1996
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Well conducted and documented study following a guideline and conducted according to GLP. Reliability rating reduced to 2 as study read across from EC 701-249-4
- Justification for type of information:
- See section 13 for attached read across justification
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- See section 13 for category and read across assessment
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure):
The test system was activated sludge collected on 16 September 1996 from the Downingtown Regional Water Pollution Control Center (DRWPCC) in Downingtown, Pennsylvania. The sludge was screened through a 2 mm sieve prior to determining the total suspended solids (TSS). Based on the TSS result approximately 1.5 liters of the sludge was adjusted to a target solids level of 2500 mg/L by diluting with tap water. The adjusted sludge was aerated in a semi-continuous activated sludge (SCAS) unit until used in the preparation of the inoculum added to all flasks.
The inoculum was prepared for addition to the CO2 flasks as follows:
Activated sludge was homogenized in a blender for ~2 minutes. The homogenized sample was poured into a beaker and allowed to settle for ~30 minutes. The supernatant was decanted and added to the flasks at a concentration of 1% v/v. On the same day, a standard plate count (SPC) was performed on the inoculum. The plates were incubated at test temperature. The result was 2.1 x 10ˆ5 CFU/mL.
- Storage conditions: Store the stock solution under refrigeration for a maximum of three days prior to test initiation. - Duration of test (contact time):
- 28 d
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
The test medium was modified BOD water containing the following standard reagent solutions per liter of water:
1 mL magnesium sulfate solution (2.25%), VWR, (Lot - 9502168)
1 mL calcium chloride solution (2.75%), VWR, (Lot - 9502027)
2 mL phosphate buffer (pH 7.2), VWR, (Lot - 9502056)
4 mL ferric chloride solution (0.025%), VWR, (Lot 9503395)
1 mL of a 4% (w/v) solution of (NH4)2SO4, EM Science, (Lot - 35065517)
- Solubilising agent (type and concentration if used): The test substance has limited solubility in water and was hence added to the appropriate test flasks at a concentration of 10 mg C/L by direct weight addition
- Test temperature: 21.6°C-23.4°C
- Aeration of dilution water: Yes, overnight aeration
- Suspended solids concentration: Based on the TSS result approximately 1.5 liters of the sludge was adjusted to a target solids level of 2500 mg/L by diluting with tap water.
TEST SYSTEM
- Culturing apparatus:
CO2-free air was provided to the test flasks through a scrubbing apparatus prepared as follows:
One empty 1-liter plastic bottle to prevent backflow into the air line.
Five 1-liter plastic bottles containing 700 mL 10 N NaOH (EM Science, Lot - 36051).
One 1-liter flask containing 700 mL 0.024 N Ba(OH)2 (to serve as a CO2 indicator trap).
One empty 1-liter plastic bottle to prevent liquid carryover into the test containers.
Six glass 4-liter Erlenmeyer flasks were used as the test vessels for this study. Duplicate flasks were required for the test substance (test suspension) and for the blank control. Single flasks were used for the reference substance and for the toxicity control. The flasks were identified by test substance identification number (TSIN), test concentration, study number, flask number, flask content code, and replicate number where applicable.
CONTROL AND BLANK SYSTEM
- Inoculum blank: Duplicate blank controls containing test medium and inoculum were included to monitor background CO2 production.
- Toxicity control: A single flask receiving sodium benzoate at a concentration of 10 mg C/L (reference substance) was included in the test - Reference substance:
- benzoic acid, sodium salt
- Test performance:
- The CO2 production from the reference chemical exceeded the 60% of theoretical necessary to consider the test valid. The %TCO2 result for the toxicity flask (IC) was > 25 as of day 12, therefore, the test substance is considered to be non-inhibitory at the concentration tested.
- Parameter:
- % degradation (CO2 evolution)
- Value:
- >= 4.7 - <= 10.8
- Sampling time:
- 28 d
- Details on results:
- Breakdown products were not noted or identified.
- Results with reference substance:
- Reference (sodium benzoate): 88.1% (0.45 day lag period)
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: Not readily biodegradable
- Conclusions:
- The test substance is not readily biodegradable.
- Executive summary:
Roy F. Weston, Inc. (WESTON.) conducted a CO2 evolution test on a test substance. This test was designed to determine the rate and extent of the ultimate biodegradation of the test substance under aerobic conditions.
The test was conducted according to the EU method: "Carbon Dioxide (CO2) Evolution (Modified Sturm Test)" Method C.4-C; ISSN 0378-6978, L 383 A, vol. 35; 29 December 1992 under conditions of GLP.
The test apparatus consisted of six glass 4-liter Erlenmeyer flasks containing two liters of modified biochemical oxygen demand (BOD) water. The test system was activated sludge collected on 16 September 1996 from the Downingtown Regional Water Pollution Control Center (DRWPCC) in Downingtown, Pennsylvania. The sludge was not exposed to the test substance in the laboratory prior to addition to the test flasks.
The test substance has limited solubility in water and was hence added to the appropriate test flasks at a concentration of 10 mg C/L by direct weight addition. A toxicity control in single replication, duplicate flasks receiving no test substance (blank control), and a single flask receiving sodium benzoate at a concentration of 10 mg C/L (reference substance) were included in the test
design. The flasks were placed on a rotary platform shaker and mixed at 110 ± 10 rpm for the duration of the study.
The CO2 produced in each flask reacted with 0.024 N Ba(OH)2 and precipitated as BaCO3. The amount of CO2 produced was determined by titrating the remaining Ba(OH)2 with 0.05 N standardized hydrochloric acid (HCl). After 28 days the contents of the flasks were acidified with concentrated sulfuric acid (H2SO4) and aerated overnight. One final titration was performed.
Based on these results the test substance would not be considered readily biodegradable under the European Economic Community (EC) criteria, which requires 60% biodegradation within 28 days, achieved within 10 days of reaching 10% biodegradation.
The CO2 evolved from the reference chemical exceeded the 60% of theoretical necessary to consider the test valid. The %TCO2 result for the toxicity flask (TC) was >25 as of day 12, therefore, the test substance is considered to be non-inhibitory at the concentration tested.
There was no available data to fulfil this endpoint for the test material and so the study was read-across from a supporting substance (EC701 -249 -4).
Referenceopen allclose all
Test material degraded 13.4% in 28 days. The reference substance, sodium benzoate, reached a level of 88.8% in the same test period.
Based on the results the test substance would not be considered readily biodegradable under the European Economic Community (EC) criteria, which requires 60% biodegradation within 28 days, achieved within 10 days of reaching 10% biodegradation.
The reference substance, sodium benzoate, degraded 88.1% in the same test period and the toxicity test showed the test substance to be non-inhibitory, so the test was valid.
Description of key information
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
Additional information
The key studies for Biodegradation are considered to have a reliability rating of 1, however these are being used as read across to a structurally similar substance (EC 701-249-4) as there was no available data to fulfil this endpoint for the test material and so the reliability rating will be reduced to 2, according to the criteria of Klimisch, 1997.
See category assessment attached in section 13 for justification of read across for this endpoint.
There are 2 studies for this endpoint which can be considered key studies:
Edwards et al, 1996 (WESTON report number: 96-043) conducted a CO2 evolution test on a test substance. 4.7 - 10.4% degradation was seen in 28 days. Based on these results the test substance would not be considered readily biodegradable.
In a study on the ready biodegradability of the test substance in a CO2 Evolution Test by Schafefer et al, 1998 (WILDLIFE INTERNATIONAL LTD. PROJECT NO.: 331E-114) the test substance can be described as not readily biodegradable under the conditions of the test and 13.4% degradation was seen in 28 days.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.