Lithium tetrafluoroborate, anhydrous

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Lithium tetrafluoroborate, anhydrous

EC Number:

238-178-9

EC Name:

Lithium tetrafluoroborate, anhydrous

Cas Number:

14283-07-9

Molecular formula:

BF4.Li

IUPAC Name:

lithium tetrafluoroborate, anhydrous

Test material form:

solid: particulate/powder

Study design

Test type:

not specified

Water media type:

other: tap water

Limit test:

no

Total exposure duration:

3 h

Test conditions

Test temperature:

19.2 – 23.1 °C (first experiment (pre-test))
19.0 – 23.0 °C (second experiment (limit test))

pH:

7.0

Details on test conditions:

Duration 3 hours
Replicates
Positive Control 1 replicate/concentration (all experiments)
Test item 1 replicate/concentration (first experiment pre-test, lower concentrations)
5 replicates/concentration (first experiment pre-test,highest concentration)
6 replicates/ concentration 1000 mg/L (second experiment limit test)
Blank Control 2 replicates before and 2 after measuring positive control and test item, respectively
Water tap water, see annex 2, page 28
Feeding nutrient solution, 16 mL/vessel
Temperature 19.2 – 23.1 °C (first experiment (pre-test))
19.0 – 23.0 °C (second experiment (limit test))

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol (CAS-No. 591-35-5)

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

3 h

Dose descriptor:

NOEC

Effect conc.:

ca. 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Effect concentrations 2

Duration:

3 h

Dose descriptor:

EC10

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Effect concentrations 3

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Details on results:

Two valid experiments were performed.

In the first experiment (Pre-Test)the test item was tested using 4 concentrations ranging from 1000 to 1 mg/L nominal concentration. Duration of the test was 3 hours. Activated sludge was used as inoculum. It was taken from a domestic sewage treatment plant and washed before usage. The dry matter was determined as 2.38 g suspended solids/L, giv-ing a concentration of 1.19 g suspended solids/L in the test.
3,5-Dichloro-phenol was used as positive control. Four concentrations were tested; an EC50 of 9.5 mg/L (95% confidence interval: not determinable) was determined, which lies within the recommended rangeof 2– 25mg/L stated in the OECD guideline.
Because no significant inhibition was observed, no additional experiment had to be per-formed. Because only 3 replicates could be evaluated instead of 5 replicates, the experi-ment was repeated.

In the second experiment (Limit Test) the test item was tested using 6 replicates for the treatment 1000 mg/L nominal concentration.

The dry matter of the inoculum was determined as 3.10 g suspended solids/L, giving a concentration of 1.55 g suspended solids/L in the test.
For the positive control an EC50 of 8.1 mg/L (95% confidence not determinable) was de-termined. The value lies within the recommended range of the OECD guideline.
The following results for the test item Lithium Tetrafluoroborate were determined:

3h NOEC = 1000 mg/L
3h EC10 > 1000 mg/L
3h EC50 > 1000 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The following results for the test item Lithium Tetrafluoroborate were determined:

3h NOEC = 1000 mg/L
3h EC10 > 1000 mg/L
3h EC50 > 1000 mg/L