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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2018
Report date:
2018

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
Lithium tetrafluoroborate, anhydrous
EC Number:
238-178-9
EC Name:
Lithium tetrafluoroborate, anhydrous
Cas Number:
14283-07-9
Molecular formula:
BF4.Li
IUPAC Name:
lithium tetrafluoroborate, anhydrous
Test material form:
solid: particulate/powder

Study design

Test type:
not specified
Water media type:
other: tap water
Limit test:
no
Total exposure duration:
3 h

Test conditions

Test temperature:
19.2 – 23.1 °C (first experiment (pre-test))
19.0 – 23.0 °C (second experiment (limit test))
pH:
7.0
Details on test conditions:
Duration 3 hours
Replicates
Positive Control 1 replicate/concentration (all experiments)
Test item 1 replicate/concentration (first experiment pre-test, lower concentrations)
5 replicates/concentration (first experiment pre-test,highest concentration)
6 replicates/ concentration 1000 mg/L (second experiment limit test)
Blank Control 2 replicates before and 2 after measuring positive control and test item, respectively
Water tap water, see annex 2, page 28
Feeding nutrient solution, 16 mL/vessel
Temperature 19.2 – 23.1 °C (first experiment (pre-test))
19.0 – 23.0 °C (second experiment (limit test))
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol (CAS-No. 591-35-5)

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
NOEC
Effect conc.:
ca. 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Duration:
3 h
Dose descriptor:
EC10
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Details on results:
Two valid experiments were performed.

In the first experiment (Pre-Test)the test item was tested using 4 concentrations ranging from 1000 to 1 mg/L nominal concentration. Duration of the test was 3 hours. Activated sludge was used as inoculum. It was taken from a domestic sewage treatment plant and washed before usage. The dry matter was determined as 2.38 g suspended solids/L, giv-ing a concentration of 1.19 g suspended solids/L in the test.
3,5-Dichloro-phenol was used as positive control. Four concentrations were tested; an EC50 of 9.5 mg/L (95% confidence interval: not determinable) was determined, which lies within the recommended rangeof 2– 25mg/L stated in the OECD guideline.
Because no significant inhibition was observed, no additional experiment had to be per-formed. Because only 3 replicates could be evaluated instead of 5 replicates, the experi-ment was repeated.

In the second experiment (Limit Test) the test item was tested using 6 replicates for the treatment 1000 mg/L nominal concentration.

The dry matter of the inoculum was determined as 3.10 g suspended solids/L, giving a concentration of 1.55 g suspended solids/L in the test.
For the positive control an EC50 of 8.1 mg/L (95% confidence not determinable) was de-termined. The value lies within the recommended range of the OECD guideline.
The following results for the test item Lithium Tetrafluoroborate were determined:

3h NOEC = 1000 mg/L
3h EC10 > 1000 mg/L
3h EC50 > 1000 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The following results for the test item Lithium Tetrafluoroborate were determined:

3h NOEC = 1000 mg/L
3h EC10 > 1000 mg/L
3h EC50 > 1000 mg/L