Ethanamine, N-ethyl-, reaction products with polyethylene-polypropylene glycol ether with trimethylolpropane (3:1) acrylate (>1 <6.5 mol EO and >1 < 6.5 mol PO)

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study without restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

1

Reference substance name:

Ethanamine, N-ethyl-, reaction products with polyethylene-polypropylene glycol ether with trimethylolpropane (3:1) acrylate (>1 <6.5 mol EO and >1 < 6.5 mol PO)

EC Number:

605-659-3

Cas Number:

173046-61-2

Molecular formula:

Mixture of C6H11O3-[C3H6O]p-[C2H4O]q-[C3H3O]r-C7H14ON and C6H11O3-[C3H6O]p-[C2H4O]q-[C3H3O]s With: 0

IUPAC Name:

Ethanamine, N-ethyl-, reaction products with polyethylene-polypropylene glycol ether with trimethylolpropane (3:1) acrylate (>1 <6.5 mol EO and >1 < 6.5 mol PO)

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Appropriate amounts of the test item were weighed separately in glass flasks partially filled with the
Elendt M7 medium and filled up with appropriate volume of the AAP medium (Table 4) [SOP/W/7]. The
mixtures were visually heterogeneous. Therefore, the mixtures and the control (the AAP medium,
1000 mL) were mechanically shaken for 24 h (room temperature, 60 revolutions per minute). After
shaking, the mixtures (visually heterogeneous) and the control were left for a settling phase (room
temperature, 2 h). Thereafter, the mixtures (visually heterogeneous) and the control were filtrated
through a conditioned nitrocellulose membrane filter (HAWG filter, 0.45 μm, Millipore), [SOP/W/37].
This is a WAF-approach followed by filtering which results in a water soluble fraction [9]. Filtration was
chosen as the separation method since centrifugation is not possible with the large volumes of test
solution. Each filtrate was visually homogeneous and transparent.

Test organisms

Test organisms (species):

Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Common name: green algae
- Strain: SAG 61.81
- Source (laboratory, culture collection): Algae Collection University of Göttingen, Germany, cultivated at the performing institute.
- Method of cultivation: Algae were transferred from agar bevels to the fresh medium (for Pseudokirchneriella subcapitata AAP medium is recommended, Appendix 2) in 250 mL Erlenmeyer flask and incubated in 21 – 24 C in constant illumination (pre-culture) [1], [SOP/W/65]. The algae culture was renewed (inoculated to a new medium) twice a week in sterile conditions [SOP/W/56, SOP/W/70].
A pre-culture with cell density of 1 x 104 per mL was renewed three days before the definitive test. The pre-culture was used for inoculation of the test concentrations and the control.

ACCLIMATION
- Culturing media and conditions (same as test or not): yes
- Any deformed or abnormal cells observed: no

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Hardness:

18.9 mg/L
as CaCO3

Test temperature:

22.4 – 23.0°C

pH:

The pH values measured at exposure initiation were in the range of 7.50 – 7.63 and at exposure
termination were in the range of 7.49 – 8.82

Salinity:

13.6 mg/L as NaHCO3

Conductivity:

The conductivity of the test medium measured
at exposure initiation in the control, before split up into replicates, was 82.5 μS/cm

Details on test conditions:

TEST SYSTEM
- Test vessel: glass Erlenmeyer flasks 250 mL
- Type (delete if not applicable): closed with air permeable stoppers
- Material, size, headspace, fill volume: 100 mL
- Aeration: none
- Initial cells density: 10E+04 cells/mL
- Control end cells density:
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 2

GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 light:dark
- Light intensity and quality:

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: spectrophotometric measurement

TEST CONCENTRATIONS
- Range finding study: yes

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

72 h

Dose descriptor:

EL50

Effect conc.:

165 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 95% confidence interval: 152 – 180

Effect concentrations 2

Key result

Duration:

72 h

Dose descriptor:

EL10

Effect conc.:

30 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 95% confidence interval: 25 – 36

Results with reference substance (positive control):

ErC50 = 2.57 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Not harmful to aquatic algae.