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EC number: 812-745-6 | CAS number: 205041-15-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2020-03-06 to 2020-05-13
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- July 1992
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- Activated sludge from the municipal wastewater treatment plant Breisgauer Bucht was used as inoculum with a concentration corresponding to 30 mg dry solids per litre. The treatment plant clarifies predominantly domestic wastewater and has a capacity of 600.000 inhabitant equivaluents. Sampling date of activated sludge was 18 March 2020. The dry solids (d.s.) content of the activated sludge was 5.2 g/L. It was determined by weight measurements after drying at 105°C for about 4 hours (mean of triplicate measurements).
- Duration of test (contact time):
- >= 28 d
- Initial conc.:
- >= 44.4 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
Mineral medium:
A: Potassium dihydrogenphosphate KH2PO4 8.50 g
Dipotassium hydrogenphosphate K2HPO4 21.75 g
Disodium hydrogenphosphate dihydrate Na2HPO4 * 2 H2O 33.40 g
Ammonium chloride NH4Cl 0.50 g
are dissolved in demineralised water and made up to 1 litre. The pH of the solution should be 7.4.
B: Calcium chloride dihydrate CaCl2 * 2 H2O 36.4 g
is dissolved in demineralised water and made up to 1 litre.
C: Magnesium sulfate heptahydrate MgSO4 * 7 H2O 22.5 g
is dissolved in demineralised water and made up to 1 litre.
D: Iron (III) chloride hexahydrate FeCl3 * 6 H2O 0.25 g
is dissolved in demineralised water, made up to 1 litre and stabilised with one drop of concentrated HCl.
- Temperature: 21.6 - 21.9 °C
TEST SYSTEM
- Culturing apparatus: The systems OxiTop® (WTW / Xylem) and Sensomat (AQUALYTIC®) were used as test systems.
- Number of culture flasks/concentration: 3
- Method used to create aerobic conditions: magnetic stirrer
SAMPLING
- Sampling frequency: 112 min
- Sampling method: pressure measurement
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control: yes
- Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- ca. 2.4
- Sampling time:
- 28 d
- Details on results:
- Degradation was 2.4% based on ThOD (NO3) and 3.2% based on ThOD (NH4), each mean of two replicates.
- Results with reference substance:
- The reference compound sodium acetate reached the pass level of 60% ThOD within 8 days.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- After 28-d incubation, the test item showed 2.4% based on ThOD (NO3) and 3.2% based on ThOD (NH4) degradation, each mean of two replicates. Therefore, the test item is not readily biodegradable.
- Executive summary:
The purpose of this study was to determine the ready biodegradability of the test item N1,N3-diallylpropane-1,3-diamine dihydrochloride. The test item was exposed to aerobic activated sludge from the aeration tank of a domestic waste water treatment plant for 28 days. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. As a reference item, sodium benzoate was tested simultaneously under the same conditions as the test item, and functioned as a procedure control.
This study was designed to comply with the following methods:
- Commission Regulation 440/2008/EC, Method C.4-D of May 30, 2008: Manometric Respirometry Test (EEC Publication No. L 142/496, May 2008)
- OECD Guideline for Testing of Chemicals No. 301 F: "Ready Biodegradability: Manometric Respirometry Test", adopted July 17, 1992
All validity criteria were fulfilled, there were no deviations from the guidelines. The degradation rate of N1,N3-diallylpropane-1,3-diamine dihydrochloride never reached 60% within the incubation period of 28 days. The mean % biodegradation and biological oxygen demand of the test item, corrected for the inoculum blank, were 2.4% (based on ThODNO3) and 3.2% (based on ThODNH4) and 34 -31 mg/L O2, respectively, over the incubation period of 28 days. Therefore, N1,N3 -diallylpropane-1,3 -diamine dihydrochloride is not readily biodegradable under these test conditions.
Reference
Test item:
In one of the replicate test vessels, a significantly higher oxygen consumption was measured, resulting in a final degradation value of >100%. At the end of the test, when the bottles were emptied, it was found that this test vessel was contaminated with organic material that had not been noticed when filling the vessels. Since the contamination was obviously the cause of the increased values, this test replicate was regarded as an outlier, and was not considered in the test evaluation.
N-determinations:
|
NO3-N [mg/L] |
NO2-N [mg/L] |
Test start |
|
|
Blank before addition of inoculum |
0.023 |
<LOD |
Blank after addition of inoculum |
0.196 |
<LOD |
Stock solution test item before addition of inoculum |
0.061 |
<LOD |
Test end |
|
|
Blank vessel 1 |
2.43 |
<LOD |
Blank vessel 2 |
2.35 |
<LOD |
Blank vessel 3 |
2.47 |
<LOD |
Test vessel 2 |
2.40 |
<LOD |
Test vessel 3 |
2.35 |
<LOD |
LODNO2-N: 0.012 mg/L
The increase of the concentration of NO3-N during the test duration indicates, that nitrification took place.
The N-determinations are not considered in the test evaluation, because only insignificant differences between test and blank vessels were observed.
pH:
The pH in the blank, test and abiotic control vessels was inside the range 6 – 8.5. In the reference vessels, the pH was above >8.5, but the oxygen consumption by the reference substance was greater than 60% of ThOD. To apply this criterion to the toxicity control is not practical.
N1,N3 -diallylpropane-1,3 -diamine dihydrochloride - Biodegradation in % of ThODNO3
|
Test item** |
Toxicity control*** |
Reference item**** |
Abiotic control |
||||
Test item [ml/164 ml] |
164 |
164 |
164 |
164 |
|
|
|
164 |
Reference item [ml/164ml] |
|
|
|
164 |
164 |
164 |
164 |
|
ThODNO3[mg/l] |
99.9 |
99.9 |
99.9 |
199.9 |
100.1 |
100.1 |
100.1 |
99.9 |
Day |
Degradation [%] |
|||||||
0 |
0.0* |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
4 |
5.7 |
0.0 |
0.0 |
31.2 |
59.5 |
59.5 |
59.5 |
0.0 |
8 |
8.5 |
0.0 |
0.0 |
38.3 |
73.5 |
73.5 |
73.5 |
0.0 |
12 |
15.1 |
6.6 |
3.8 |
43.2 |
83.2 |
80.2 |
83.2 |
0.0 |
16 |
28.3 |
5.7 |
0.0 |
43.8 |
82.4 |
82.4 |
82.4 |
0.0 |
20 |
67.2 |
4.7 |
-3.8 |
43.5 |
80.8 |
80.8 |
80.8 |
0.0 |
24 |
88.1 |
2.8 |
-5.7 |
44.0 |
78.9 |
78.9 |
81.9 |
0.0 |
28 |
108.9 |
3.8 |
1.0 |
47.4 |
82.7 |
85.7 |
85.7 |
0.0 |
* This replicate was an outlier with >100% degradation. At the end of the study it was discovered, that the test vessel had been contaminated with organic substances. Therefore, this replicate was not considered for analysis of biodegradation of the test item.
** stock solution with 44.4 mg/L test item
***stock solution with 44.4 mg/L test item and 128.2 mg/L reference item
****stock solution with 128.2 mg/L reference item
N1,N3 -diallylpropane-1,3 -diamine dihydrochloride - Biodegradation in % of ThODNH4
|
Test item** |
Toxicity control*** |
Reference item**** |
Abiotic control |
||||
Test item [ml/164 ml] |
164 |
164 |
164 |
164 |
|
164 |
||
Reference item [ml/164ml] |
|
164 |
164 |
164 |
164 |
|
||
ThODNH4[mg/l] |
75.0 |
75.0 |
75.0 |
175.0 |
100.1 |
100.1 |
100.1 |
75.0 |
Day |
Degradation [%] |
|||||||
0 |
0.0* |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
4 |
7.6 |
0.0 |
0.0 |
35.6 |
59.5 |
59.5 |
59.5 |
0.0 |
8 |
11.3 |
0.0 |
0.0 |
43.7 |
73.5 |
73.5 |
73.5 |
0.0 |
12 |
20.1 |
8.8 |
5.0 |
49.3 |
83.2 |
80.2 |
83.2 |
0.0 |
16 |
37.7 |
7.6 |
0.0 |
50.0 |
82.4 |
82.4 |
82.4 |
0.0 |
20 |
89.4 |
6.3 |
-5.1 |
49.6 |
80.8 |
80.8 |
80.8 |
0.0 |
24 |
117.3 |
3.7 |
-7.6 |
50.3 |
78.9 |
78.9 |
81.9 |
0.0 |
28 |
145.0 |
5.1 |
1.3 |
54.2 |
82.7 |
85.7 |
85.7 |
0.0 |
* This replicate was an outlier with >100% degradation. At the end of the study it was discovered, that the test vessel had been contaminated with organic substances. Therefore, this replicate was not considered for analysis of biodegradation of the test item.
** stock solution with 44.4 mg/L test item
***stock solution with 44.4 mg/L test item and 128.2 mg/L reference item
****stock solution with 128.2 mg/L reference item
Description of key information
The purpose of this study was to determine the ready biodegradability of the test item N1,N3-diallylpropane-1,3-diamine dihydrochloride. The test item was exposed to aerobic activated sludge from the aeration tank of a domestic waste water treatment plant for 28 days. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. As a reference item, sodium benzoate was tested simultaneously under the same conditions as the test item, and functioned as a procedure control.
This study was designed to comply with the following methods:
- Commission Regulation 440/2008/EC, Method C.4-D of May 30, 2008: Manometric Respirometry Test (EEC Publication No. L 142/496, May 2008)
- OECD Guideline for Testing of Chemicals No. 301 F: "Ready Biodegradability: Manometric Respirometry Test", adopted July 17, 1992
All validity criteria were fulfilled, there were no deviations from the guidelines. The degradation rate of N1,N3-diallylpropane-1,3-diamine dihydrochloride never reached 60% within the incubation period of 28 days. The mean % biodegradation and biological oxygen demand of the test item, corrected for the inoculum blank, were 2.4% (based on ThODNO3) and 3.2% (based on ThODNH4) and 34 -31 mg/L O2, respectively, over the incubation period of 28 days. Therefore, N1,N3-diallylpropane-1,3-diamine dihydrochloride is not readily biodegradable under these test conditions.
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
- Type of water:
- freshwater
Additional information
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