Fats and Glyceridic oils, shea butter, unsaponifiable fraction

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

30.11.2012-28.12.2012

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2012

Report date:

2012

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Version / remarks:

SS-EN ISO 9408:2000/OECD 301 F

Deviations:

yes

Remarks:

Minor

GLP compliance:

no

Remarks:

It is assumed that the study was not perfomed according to GLP

Test material

Test material information

Constituent 1

Reference substance name:

Fats and Glyceridic oils, shea butter, unsaponifiable fraction

EC Number:

607-097-4

Cas Number:

225234-14-0

Molecular formula:

UVCB substance

IUPAC Name:

Fats and Glyceridic oils, shea butter, unsaponifiable fraction

Specific details on test material used for the study:

Commercial name of the registered substance "Lipex Shea Tris" has been used in the test report.
Batch number: 1322716

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

Activated sludge from Kavlinge wastewater plant, Sweden, was used as inoculum. The inoculum was nor pre-adapted. The final concentration of suspended solids in the mediums was 30 mg/l.

Duration of test (contact time):

ca. 28 d

Initial test substance concentration

Initial conc.:

ca. 78 mg/L

Based on:

ThOD

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

A measured volume of inoculated mineral medium, containing a known concentration of test substance (100 mg test substance/l giving at leats 50-100 mg Theoretical Oxygen Deman, ThOD/l) as the nominal sole source of organic carbon, is tirred in a closed flask at a constant temperature for 28 days. The consumption of oxygen is determined from the change in pressure in the apparatus. Evolved carbon is absorbed in a solution of potassium hydroxide. The amount of oxygen taken up by the microbial population during biodegradation of the test substance (corrected for uptake by blank inoculum, run in parallel) is expressed as a percentage of ThOD or Chemical Oxygen Demand (COD).

Reference substance

Reference substance:

other: Sodium acetate

Results and discussion

% Degradation

% Degradation 1

Key result

Parameter:

other: ThOD

Value:

69

Sampling time:

10 d

% Degradation 2

Key result

Parameter:

other: ThOD

Value:

105

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

Not included

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

The test article "Lipex Shea Tris" was degraded by approximately 69% within the 10-day window and by approximately 105% after 28 days.

The test article "Lipex Shea Tris" can be regarded as readily biodegradable, subject to the COD measurement.

Executive summary:

According to SS-EN ISO 9 408:2000, the test is valid if the degradation of the reference compound in the control bottle exceeds 50% after 5 days, if the degradation of the reference compound exceeds 40% in the inhibition bottle and if the pH values in the test bottles are between 6 and 8. All these criteria are fulfilled.

According to OECD guidelines for testing chemicals a test compound is regarded as readily biodegradable if the removal of ThOD within 28 days is greater than 60%. The pass value has to be reached in a 10-day window within the 28-day period of the test. The 10-day window begins when the degree of biodegradation has reached 0% ThOD (or COD) and must end before day 28 of the test.

The test article “Lipex Shea Tris” reached a biodegradation of 105% af 28 days and can be regarded as readily biodegradable, subject to the COD measurement.