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Diss Factsheets
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EC number: 254-159-8 | CAS number: 38861-78-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 1-[4-(2-methylpropyl)phenyl]ethan-1-one
- EC Number:
- 254-159-8
- EC Name:
- 1-[4-(2-methylpropyl)phenyl]ethan-1-one
- Cas Number:
- 38861-78-8
- Molecular formula:
- C12H16O
- IUPAC Name:
- 1-[4-(2-methylpropyl)phenyl]ethan-1-one
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Samples from all controls and treatment groups were taken at the test start and at the test end.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The definitive test was performed using the test item loading rate of 60 mg/L, and 2.5-fold, 6.25-fold, 15.63-fold, 39.06-fold diluted filtrates of the loading rate of 60 mg/L plus the filtrate of the control.
Appropriate amount of the test item was weighed in a glass crystallizer, quantitatively transferred into a glass vessel and filled up to full volume (without headspace) with the AAP medium. The glass vessel was tightly closed with a glass cap.
The loading rate of 60 mg/L was visually heterogeneous with visible undissolved particles. The test item loading rate and the control (the AAP medium, 35 L) were mechanically shaken for 24 h (room temperature, 50 revolutions per minute). After 24 h of mechanical shaking, the loading rate (heterogeneous) and the control were stored at room temperature for a settling phase (no shaking). After 24 h of settling phase, the loading rate was still visually heterogeneous. Therefore, the loading rate and the control were filtered through a conditioned nitrocellulose membrane filter (0.22 µm, GSTF, Millipore), [SOP/W/37]. This is a WAF-approach followed by filtering, which results in a water soluble fraction. Each filtrate was visually homogeneous and transparent. The filtrate of the loading rate of 60 mg/L was sequentially diluted with filtrate of the control to prepare the lower test item concentrations.
All exposure levels and the control were inoculated with the algal pre-culture to obtain the initial algal density of 0.01 x 106 cells/mL.
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Green algae
- Source (laboratory, culture collection): Unicellular freshwater green algae Pseudokirchneriella subcapitata (Reinsch) Korshikov (syn. Selenastrum capricornutum Prinz, Raphidocelis subcapitata Korshikov), specification SAG 61.81, cultured in the Institute of Industrial Organic Chemistry, Branch Pszczyna, Department of Ecotoxicology, Laboratory of Aquatic Toxicology, stock from the Culture Collection of Algae at Göttingen University, Germany.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 21.7 - 22.1 °C
- pH:
- 7.43 - 8.81
- Conductivity:
- 139.6 µS/cm
- Nominal and measured concentrations:
- Nominal: 0, filtrate of the test item loading rate of 60 mg/L and 2.5-fold, 6.25-fold, 15.63-fold,and 39.06-fold diluted filtrates of the loading rate of 60 mg/L.
Measured: < 0.18 (control); 0.73, 1.69, 4.05, 9.46, 24.54 mg/L
Analytical measured concentrations were within 89.6 – 97.5% of the initial concentrations in all treatment groups. - Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flasks
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 250 ml
- Aeration: no
- Initial cells density: 1E+04
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to OECD 201
- Intervals of water quality measurement: daily
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: no
- Photoperiod: cointinuous illumination
- Light intensity and quality: 7000 – 7494 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : cell density after 24, 48 and 72 h
- Determination of cell concentrations: [counting chamber; electronic particle counter; fluorimeter; spectrophotometer; colorimeter]Countng, indiecte spectophotometric measurements at 670 nm.
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 5.11 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.69 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Results with reference substance (positive control):
- 72 h ErC50: 3.23 mg/L
- Reported statistics and error estimates:
- Probit method calculations and analysis by Shapiro-Wilk’s Test on Normal Distribution, Levene’s Test on Variance Homogeneity (with Residuals), Williams Multiple Sequential t-test Procedure.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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