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Diss Factsheets
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EC number: 209-665-3 | CAS number: 589-92-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Repeated dose toxicity: inhalation
Administrative data
- Endpoint:
- short-term repeated dose toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- significant methodological deficiencies
- Remarks:
- Insufficient information on substance identity and purity.
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 943
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Version / remarks:
- The study was conducted before OECD test guidelines and GLP were established.
- Principles of method if other than guideline:
- Test animals were exposed in metal cages or plywood cages. The test air was mixed with a measured amount of test substance vapour, and was then passed into the top of a cage and was thoroughly distributed in the chamber. The flow rate of air ranged from 300 to 800 liters per minute, resulting in one or two air changes per minute. Animals were exposed whole body for 6 hours per day, 5 days per week during a period of 3 or 10 weeks.
- GLP compliance:
- no
- Limit test:
- no
Test material
- Reference substance name:
- 4-methylcyclohexanone
- EC Number:
- 209-665-3
- EC Name:
- 4-methylcyclohexanone
- Cas Number:
- 589-92-4
- Molecular formula:
- C7H12O
- IUPAC Name:
- 4-methylcyclohexan-1-one
- Reference substance name:
- 3-methylcyclohexanone
- EC Number:
- 209-710-7
- EC Name:
- 3-methylcyclohexanone
- Cas Number:
- 591-24-2
- Molecular formula:
- C7H12O
- Test material form:
- liquid
- Details on test material:
- - Density: 0.914
- Boiling point: 169-170.5°C
- Refractive index: 1.4458
Constituent 1
Constituent 2
Test animals
- Species:
- rabbit
- Strain:
- not specified
- Sex:
- not specified
- Details on test animals or test system and environmental conditions:
- Young, healthy rabbits were used in the tests.
Administration / exposure
- Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- whole body
- Vehicle:
- clean air
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: metal or plywood chambers
- Method of holding animals in test chamber: not specified
- Source and rate of air: drawn from an indoor floor
- Method of conditioning air: filtered through a steel filter and passed through an electric precipitator, then passed over a cooling coil and afterwards over a steam reheating coil before entering the tubes leading from a main duct to the individual test chambers; before entering a cage the air was mixed with a measured amount of test substance vapour that was generated by pumping defined amounts of liquid substance into a glass well and the substance was evaporated at the same rate as liquid substance was delivered by the pump.
- Temperature, humidity, pressure in air chamber: 24 °C (22 to 25.5 °C), 45% humidity
- Air flow rate: 350 to 800 L/min
- Air change rate: 1 to 2 per minute
- Treatment of exhaust air: exhaust air was removed by an orifice at the rear of a cage and not re-circulated into a test chamber.
TEST ATMOSPHERE
- Brief description of analytical method used: the test substance in the air was determined colorimetrically by measuring the intensity of pink colour produced by a Zimmerman typ reaction with m-dinitrobenzene.
- Samples taken from breathing zone: no - Analytical verification of doses or concentrations:
- yes
- Details on analytical verification of doses or concentrations:
- Samples of air from the test chamber were drawn by using an evacuated 1L glass bulb that was placed in the cage for 3 minutes. Absolute ethyl alcohol was used to extract the test substance from the air. An aliquot of 10 mL of the alcoholic extract was filled into 25 mL volumetric flask and 1 mL of 1% m-dinitrobenzene in absolut alcohol was added, followed by 10 mL water. The sample was incubated at 60 °C for 15 minutes, then 2 mL of 0.3N aqueous potassium hydroxide were added and the sample incubated at 60 °C for 10 minutes. It was then refrigerated at 4-5 °C for 12 to 18 hours before being diluted to 25 mL with 50% ethyl alcohol. The sample was then compared with a freshly prepared standard in the cell of a wedge photometer at 540 nm.
- Duration of treatment / exposure:
- 3 weeks for two higher doses, 10 weeks for two lower doses
6 hours per day - Frequency of treatment:
- 5 days per week
Doses / concentrationsopen allclose all
- Dose / conc.:
- 0.82 mg/L air (nominal)
- Dose / conc.:
- 2.31 mg/L air (nominal)
- Dose / conc.:
- 5.12 mg/L air (nominal)
- Dose / conc.:
- 8.19 mg/L air (nominal)
- No. of animals per sex per dose:
- 4
- Control animals:
- yes, concurrent no treatment
Examinations
- Observations and examinations performed and frequency:
- Body weight was determined daily prior to exposure, during the period of exposure and for at least two monther after the exposure period.
Erythrocytes, leucocytes and various types of leucocytes were counted weekly and haemoglobin concentration was measured weekly prior to exposure, during the period of exposure and for at least two monther after the exposure period. - Sacrifice and pathology:
- Surviving animals were studied for 2 months after exposure ended. Animals were then sacrificed and a detailed gross and microscopic pathologic examination was done.
- Statistics:
- None
Results and discussion
Results of examinations
- Clinical signs:
- effects observed, treatment-related
- Description (incidence and severity):
- Lethargy, distention of the superficial veins of the ear, and salivation during exposure to the two higher concentrations.
- Mortality:
- no mortality observed
- Body weight and weight changes:
- not specified
- Description (incidence and severity):
- The animals had an increase in body weight during the exposure period. The highest average gain in any group was 1105 g per rabbit.
- Ophthalmological findings:
- effects observed, treatment-related
- Description (incidence and severity):
- Signs of conjunctival irritation during exposure to the two higher concentrations.
- Haematological findings:
- no effects observed
- Clinical biochemistry findings:
- not examined
- Urinalysis findings:
- not examined
- Behaviour (functional findings):
- not examined
- Immunological findings:
- not examined
- Organ weight findings including organ / body weight ratios:
- not specified
- Gross pathological findings:
- not specified
- Neuropathological findings:
- not examined
- Histopathological findings: non-neoplastic:
- not examined
- Histopathological findings: neoplastic:
- not examined
Target system / organ toxicity
- Critical effects observed:
- no
- Lowest effective dose / conc.:
- 2.31 mg/L air (nominal)
- System:
- eye
Any other information on results incl. tables
A light form of conjunctival irritation, apparent as reddening, was observed at an exposure concentration of 2.31 mg/L. There was no evidence of adverse effects at the exposure concentration of 0.82 mg/L.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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