2-(oxolan-2-ylmethoxy)ethanol

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

05 Jul - 08 Aug 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Version / remarks:

1992

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)

Version / remarks:

2008

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

Hessisches Ministerium für Umwelt, Klimaschautz, Landwirtschaft und Verbraucherschutz, Wiesbaden, Germany

Test material

Test material information

Constituent 1

Reference substance name:

2-[(tetrahydrofurfuryl)oxy]ethanol

EC Number:

227-407-8

EC Name:

2-[(tetrahydrofurfuryl)oxy]ethanol

Cas Number:

5831-59-4

Molecular formula:

C7H14O3

IUPAC Name:

2-(tetrahydrofuran-2-ylmethoxy)ethanol

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge: Aerobic activated sludge from sewage treatment plant Bensheim, Germany
- Storage conditions: aerated
- Storage length: sludge was stored overnight
- Preparation of inoculum for exposure: Activated sludge was washed by centrifugation and the supernatant liquid phase was decanted; solid material was resuspended in test water and centrifuged again and repeated three times; based on the the ration of wet sludge to its dry weight calculated aliquots of washed sludge suspension, corresponding to 3.5 g dry material per litre were mixed with test water and aerated overnight.
- Pretreatment: An aliquot of sludge suspension was weighed, dried and the ratio of wet sludge to its dry weight was determined.
- Concentration of sludge: 3.5 g dry material per litre

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

101.2 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

167 mg/L

Based on:

COD

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium: according the OECD guideline 301
- Test temperature: 22 ± 1°C
- pH: 7.3 to 7.7
- pH adjusted: yes, adjusted with 1 N HCl to pH 7.5
- Aeration of dilution water: Aeration overnight
- Continuous darkness: yes
- Other: continuous stirring

TEST SYSTEM
- Culturing apparatus and measuring equipment: BSB/BOD-Sensor-System, Aqualytic Dortmund, Germany (Manometric Test System, 500 mL fill volume in flasks)
- Number of culture flasks/concentration: 2
- Details of trap for CO2: potassium hydroxide solution (45%)

SAMPLING
- Sampling method: change of pressure in the test flasks was measured continuously by means of a manometric method

CONTROL AND BLANK SYSTEM
- Inoculum control: 2 replicates
- Abiotic sterile control: 1 replicate (poisoned with HgCl2)
- Toxicity control: 1 replicate
- Other: procedure control - 1 replicate

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

49

Sampling time:

28 d

Details on results:

Test item is not inhibitory to aerobic activated sludge microorganisms since degradation was >25% within 14 days (56% after 14 d and 63% after 28 d) in the toxicity control.

BOD5 / COD results

Results with reference substance:

The reference item was sufficiently degraded to 82% after 14 days and to 88% after 28 days of incubation.

Any other information on results incl. tables

Table 1: Cumulative Biochemical Oxygen Demand (mg O2/L) in Test Flasks during the Test Period of 28 Days

Time	Flask No.
(days)	1	2	3	4	5	6	7
1	0	0	0	0	55	0	60
2	5	5	5	5	80	0	80
3	5	5	5	5	120	0	120
4	10	20	5	10	130	0	130
5	30	35	5	10	135	0	130
6	40	45	5	10	140	0	145
7	45	50	5	10	145	0	160
8	55	55	5	10	145	0	170
9	65	60	5	15	150	0	175
10	80	70	5	15	150	0	185
11	80	75	5	15	155	0	190
12	85	80	10	15	155	0	190
13	85	80	10	15	155	0	195
14	85	80	10	15	155	0	200
15	85	80	10	15	155	0	200
16	85	85	10	15	155	0	205
17	90	85	10	15	160	0	205
18	90	85	10	15	160	0	210
19	90	85	10	15	160	0	210
20	90	85	10	15	160	0	210
21	90	90	10	15	160	0	215
22	95	90	10	15	160	0	215
23	95	90	10	15	160	0	220
24	95	90	10	15	160	0	220
25	95	90	10	15	160	0	220
26	95	95	10	15	165	0	225
27	95	95	10	15	165	0	225
28	95	95	10	15	165	0	225

 Flasks 1 and 2: Glycofurol

 Flasks 3 and 4: inoculum control

 Flask 5: reference (procedure control)

 Flask 6: abiotic control

 Flask 7: toxicity control

Table 2: Validity criteria

Criterion from the guideline	Outcome	Validity criterion fulfilled
Difference of extremes of replicate values of the removal of the test chemical at the plateau, at the end of the test or at the end of the 10-d window, as appropriate, is less than 20%.	0%	yes
Percentage degradation of the reference compound has reached the pass levels by day 14.	82%	yes
The oxygen uptake of the inoculum blank is normally 20-30 mg O2/L and should not be greater than 60 mg/L in 28 days.	12.5 mg/L	yes

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable