Amines, N-coco alkyltrimethylenedi-, acetates

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

02 February 2007 to 02 March 2007

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Qualifier:

according to guideline

Guideline:

OECD Guideline 306 (Biodegradability in Seawater)

Deviations:

no

GLP compliance:

yes

Oxygen conditions:

aerobic

Inoculum or test system:

natural water: marine

Details on inoculum:

- Natural seawater was collected from Anglesey Sea Zoo in North Wales on 29 January 2007.
- Temperature at collection: 13.1 °C
- pH at collection: 7.84
- Salinity at collection: 31.9 g/L
- Dissolved oxygen at collection: 100.0%
- After collection, the seawater was course filtered and maintained in the dark.
- Seawater was aged prior to use by gentle aeration at 15 ± 2 °C

Duration of test (contact time):

28 d

Initial conc.:

>= 1.5 - <= 3.5 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

METHODS
- Test medium: Prepared according to the method detailed in STL Runcorn SOP III.36
- Test material was prepared on the initial day of the study.
- Test vessels: Completely filled sealed glass BOD bottles of nominal 272 mL volume.
- Test conditions: All test bottles contained course filtered, natural seawater as inoculum.
- For each of the sample days, duplicate bottles were prepared for each of the test material concentrations and reference material.
- Three replicates for the control vessel were prepared.
- During the study, all bottles were incubated at 16.5 to 17.5 °C in the dark.

ANALYSIS OF STUDY DATA
- The calculated COD value and dissolved oxygen data recorded at each analysis point (including Day 0 readings) were processed to derive the percentage degradability of the test material.
- Degradation values were calculated using the equation % degradability = [(BOD mgO2/mg test material / COD mgO2/mg) * 100]

Reference substance:

benzoic acid, sodium salt

Remarks:

2.5 mg/L

Parameter:

% degradation (O2 consumption)

Value:

71.1

Sampling time:

28 d

Remarks on result:

other: 1.5 mg/L test item

Key result

Parameter:

% degradation (O2 consumption)

Value:

70.3

Sampling time:

28 d

Remarks on result:

other: 3.5 mg/L test item

Details on results:

- Using the COD equation in STL Runcorn SOP III.1, the chemical oxygen demand was calculated as 1.931 mg/mg
- Percentage degardability of the test material and reference substance is given in the summary table (attached).
- Degradation profiles for the test item and reference substance are shown in Figure 1 (attached).
- Results of the dissolved oxygen measurements are shown in Table 1 (attached).

Key result

Parameter:

COD

Value:

1.931 other: mg/mg

Results with reference substance:

- A degradation figure of 96.5% was obtained after 28 days for the reference material.
- The inoculum was thus demonstrated to be biologically active.

Validity criteria fulfilled:

not specified

Interpretation of results:

readily biodegradable

Conclusions:

The test item showed high potential for degradation at concentrations of 1.5 mg/L and 3.5 mg/L in the marine environment.

Executive summary:

The test item was assessed for rate and extent of biodegradation when exposed to marine microorganisms for 28 days in accordance with OECD 306. The substance showed high potential for degradation at concentrations of 1.5 mg/L and 3.5 mg/L.

Description of key information

The test item was assessed for rate and extent of biodegradation when exposed to marine microorganisms for 28 days in accordance with OECD 306. The substance showed high potential for degradation at concentrations of 1.5 mg/L and 3.5 mg/L (OECD 306).

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable

Additional information

Dissolved oxygen concentration removal of 74% was demonstrated in nutrient fortified seawater with a test item concentration of 3.5 mg/L using the closed bottle method (OECD 306). In line with ECHA Guidance on Information Requirements and Chemical Safety Assessment Chapter R.7b: Endpoint specific guidance (Version 4.0; June 2017), the test substance is therefore considered likely to fulfil the criteria for ready biodegradability.