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EC number: 229-565-3 | CAS number: 6613-64-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017-11-21 - 2017-11-23
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- OECD Guideline for Testing of Chemicals No. 202, adopted 13. Apr. 2004: ”Daphnia sp., Acute Immobilisation Test“
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- Council Regulation (EC) No. 440/2008, Method C.2. “Daphnia sp. Acute Immobilisa-tion Test”, adopted 30. May 2008
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: OECD guidance document no. 23, GUIDANCE DOCUMENT ON AQUATIC TOXICITY TESTING OF DIFFICULT SUBSTANCES AND MIXTURES, adopted 14. Dec. 2000
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 1-(3-sulphonatopropyl)-2-vinylpyridinium
- EC Number:
- 229-565-3
- EC Name:
- 1-(3-sulphonatopropyl)-2-vinylpyridinium
- Cas Number:
- 6613-64-5
- Molecular formula:
- C10H13NO3S
- IUPAC Name:
- 3-(2-vinylpyridinium-1-yl)propane-1-sulfonate
- Test material form:
- solid: particulate/powder
- Remarks:
- light beige
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: sponsor
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: The test item was stored dark and dry in a closed vessel at room temperature (15.8 - 22.5°C).
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0, 4.6, 10, 22, 46, 100 mg/l
- Sampling method: 500 µL of samples in aqueous media were mixed with 500 µL acetonitrile in a vial each. Filtration using 0.45 µm Nylon filters is possible.
Test solutions
- Vehicle:
- yes
- Remarks:
- dilution water
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A solution containing 101 mg/L in dilution water was prepared. The solution was used as highest treatment and for preparation of lower concentrations by dilution with dilution water.
- Controls: yes, concurrent dilution water (negative control)
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): none stated
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain/clone: Daphnia magna STRAUS Berlin
- Source: Origin: Umweltbundesamt Berlin, In-house breeding since 27. September 2007
- Feeding during test: no
- Type and amount of food under culturing conditions: unicellular green algae (Desmodesmus subspicatus)
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES:
Selection of Daphnia
23 hours before the start of the test, the adult animals were separated from the young. 40 minutes before test start, the adults were caught with the help of a glass tube, and the newborn daphnia, aged between 0 and 22 hours 20 minutes, were sieved from the medium and immediately placed into a beaker containing dilution water. After a settling-in period of 40 minutes, animals which showed no apparent damage were used for the test.
Switching from M4-medium (husbandry) to dilution water (test) has been shown not to cause any detrimental effects for test daphnia
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- none
Test conditions
- Hardness:
- 2.502 mmol/l / 250 mg CaCO3/l
- Test temperature:
- 19.7 - 20.9°C
- pH:
- 7.8
- Dissolved oxygen:
- After preparation, the dilution water was aerated; 8.5 - 9.1 mg/l
- Nominal and measured concentrations:
- Nominal: 0, 4.6, 10, 22, 46, 100 mg/l
Measured, at 0h: 0, 4.84, 10.20, 21.90, 45.38, 98.38 mg/l
Measured, at 48h: 0, 4.48, 10.31, 23.12, 48.92, 107.77 mg/ml
The concentrations to be tested are based on the result of a non-GLP pre-test. - Details on test conditions:
- TEST SYSTEM
- Test vessel: Beakers, glass, nominal volume 50 mL, containing each 20 ± 5 mL test solution
- Type (delete if not applicable): open
- Aeration: dilution water was aerated
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Chlorine: 293.80 mg/l CaCl2*2H2O, 5.80 mg/l KCl
- Ca/mg ratio: 293.80 mg/l CaCl2*2H2O, 123.30 mg/l MgSO4*7H2O
- Culture medium different from test medium: yes
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16/8 hours, using neon tubes
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mobility daily, pH, oxygen content at 0 and 48h
VEHICLE CONTROL PERFORMED: yes - Reference substance (positive control):
- yes
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 95.94 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 46 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Mortality of control: none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: none
- Effect concentrations exceeding solubility of substance in test medium: none - Results with reference substance (positive control):
- The 24h-EC50 value was determined as 1.6 mg/L.
The value was within the requested range for the 24h-EC50 between 0.6 - 2.1 mg/L of potassium dichromate K2Cr2O7 as specified in the OECD guideline. Since the fits showed sufficient statistical correspondence of the data with the dose-response-equation, this reference study can be considered valid.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The study was conducted under GLP according to OECD guideline 202 and EU method C.2 on the registered substance itself. The method is to be considered scientifically reasonable with no deficiencies in documentation or deviations from the guidelines, the validity criteria were met. Hence, the results can be considered as reliable to assess the toxicity of SPV towards daphnids.
The toxicity against Daphnia magna was tested in a static test design using 5 concentrations ranging from 4.6 to 100 mg/L. The analytical determinations of the test item showed very good correlation between nominal and measured concentrations and very good recovery rates. Therefore, the test item 1-(3-Sulfopropyl)-2-vinyl-pyridinumbetain (SPV) can be stated as stable under the test condition and the determination of the results was based on the nominal concentrations.
Only in the highest concentration 60% immobilisation was observed, the EC50 was determined to be 95.94 mg/l. Based on the obtained results, SPV does not need to be classified as acutely toxic to the environment, but as it is not biodegradable it should be classified as hazardous to the aquatic environment Cat. 3 (chronic) according to the Regulation (EC) No. 1272/2008. - Executive summary:
The study was conducted under GLP according to OECD guideline 202 and EU method C.2 on the registered substance itself.
One valid experiment was performed.
The study was performed using 5 concentrations ranging from 4.6 to 100 mg/L. For each test concentration, 20 daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised daphnia were counted.
Only in the highest concentration 60% immobilisation was observed. None of the animals was immobilised in the blank control.
Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a current reference study to assure that the test conditions are reliable.
At the beginning and at the end of the test, the content of the test item in the test solutions was determined using HPLC-UV-determination. The concentrations determined at the start of the test were in a range from 98.38 to 105.17% of the nominal concentration. At the end of the test the determined concentrations were between 97.3 and 107.77% of the nominal concentration. The analytical determinations of the test item showed very good correlation between nominal and measured concentrations and very good recovery rates. Therefore, the test item 1-(3-Sulfopropyl)-2-vinyl-pyridinumbetain (SPV) can be stated as stable under the test conditions. Therefore, the determination of the results was based on the nominal concentrations.
The following results were determined for the test item 1-(3-Sulfopropyl)-2-vinyl-pyridinumbetain (SPV) (species: Daphnia magna):
48h-NOEC = 46 mg/L
48h-LOEC = 100 mg/L
24h-EC50 > 100 mg/L
48h-EC50 = 95.94 mg/L
SPV should be classified as hazardous to the aquatic environment Cat. 3 (chronic) according to Regulation (EC) No. 1272/2008.
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