Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 205-748-3 | CAS number: 149-87-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 08 December 1992 to 22 January 1993
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Study is a non-GLP study conducted in accordance with generally accepted scientific principles, possibly with incomplete reporting or methodological deficiencies, which do not affect the quality of the relevant results. The original full report is in Japanese and has been translated into English.
- Reason / purpose for cross-reference:
- other: Read-across target
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- Deviations:
- no
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: Standard activated sludge was obtained from Chemicals Inspection & Testing Institute.
- Preparation of inoculum for exposure: The standard activated sludge was suspended in ion exchange water and centrifuged. Washing was repeated three times. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: See the field “Any other information on materials and methods incl. tables”
- Concentration of activated sludge: 30 mg/L (as dry weight)
- Test temperature: 25 ± 1 °C
- Continuous darkness: Yes
TEST SYSTEM
- Number of culture flasks/concentration: Duplicate flasks
- Measuring equipment: Oxygen consumption was continuously measured by BOD measuring equipment
- Measurement conditions: Measurement range, 250 ppm (full scale, 100 % = 75 mgO); Chart speed, 3.125 mm/h; Volume of incubation liquid, 300 mL.
- Test performed in closed vessels: Yes, the inoculum was incubated by stirring in a closed system
SAMPLING
- Sampling frequency: All test systems were measured after Day 7, 14, 21 and 28.
CONTROL AND BLANK SYSTEM
- Inoculum blank: Activated sludge + culture medium
- Abiotic sterile control: Test material + ion exchange water
- Reference substance: Aniline + activated sludge + culture medium
- Sludge blank: Activated sludge + culture medium - Reference substance:
- aniline
- Remarks:
- 100 mg/L
- Parameter:
- % degradation (O2 consumption)
- Value:
- 74.4
- Sampling time:
- 28 d
- Results with reference substance:
- The degree of degradation in the aniline treated test system was > 40% after 7 days, confirming that the test system was valid.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Under the conditions of the study, the test material degraded by 74.4 % after 28 days and was determined to be readily biodegradable.
- Executive summary:
The biodegradation of the test material was determined in a ready biodegradability (modified MITI Test (I)) performed according to the OECD guideline 301.C. Standard activated sludge was used as the source of microorganisms, and biochemical oxygen demand (BOD) was continuously measured by closed system automatic measuring equipment of consumed oxygen. Under the conditions of the test, the test material degraded by 74.4% after 28 days and was determined to be readily biodegradable.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study conducted on read-across material
- Justification for type of information:
- Read-across from a structurally similar substance. Please refer to the RAAF report (section 13) for further information/ justification.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 74.4
- Sampling time:
- 28 d
Referenceopen allclose all
Table 1: Degree of Biodegradation (%)
Test System |
After Day 7 |
After Day 14 |
After Day 21 |
After Day 28 |
After Day 28 (average) |
Test Material, Replicate A |
62.3 |
67.9 |
75.4 |
79.3 |
74.4 |
Test Material, Replicate B |
62.3 |
70.7 |
72.6 |
72.6 |
|
Abiotic Sterile Control |
58.6 |
62.3 |
65.1 |
67.0 |
- |
Reference Substance* |
66.6 |
78.4 |
89.0 |
95.3 |
- |
* Aniline
Table 2: BOD and TOD Values for the Added Amount of Test Material and Aniline (mgO)
Test System |
After Day 7 |
After Day 14 |
After Day 21 |
After Day 28 |
TOD Value |
Test Material, Replicate A |
27.8 |
33.8 |
39.4 |
42.0 |
40.3 |
Test Material, Replicate B |
27.8 |
34.9 |
38.2 |
40.5 |
40.3 |
Abiotic Sterile Control |
23.6 |
25.1 |
26.2 |
27.0 |
40.3 |
Reference Substance* |
66.0 |
81.0 |
93.8 |
102.0 |
95.2 |
Inoculum Blank |
2.6 |
6.4 |
9.0 |
11.2 |
- |
* Aniline
Description of key information
Read-across to a substance with a similar structure.
Readily Biodegradable, 74.4 % after 28 days, OECD 301 C, Yoshikawa (1993).
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
Read-across to a substance with a similar structure.
The key study Yoshikawa (1993) is a non-GLP study yet was conducted in accordance to the standardised guideline the OECD 301.C. The original full study report is in Japanese and has been translated into English. Sufficient details were reported to assess the quality of the submitted results; the study has been assigned a reliability score of 2 in line with the principles for assessing data quality as defined by Klimisch (1997).
The biodegradation of the test material was determined in a ready biodegradability (modified MITI Test (I)) performed according to the OECD guideline 301.C. Standard activated sludge was used as the source of microorganisms, and biochemical oxygen demand (BOD) was continuously measured by closed system automatic measuring equipment of consumed oxygen. Under the conditions of the test, the test material degraded by 74.4% after 28 days and was determined to be readily biodegradable.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.