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EC number: 283-640-5 | CAS number: 84696-21-9 Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Hydrocotyle asiatica, Umbelliferae.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From October 15 to October 17, 2019
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Test Item name: Centella asiatica dry extract
Batch No.: 972/43/A
Appearance: Dark green powder - Analytical monitoring:
- yes
- Vehicle:
- yes
- Remarks:
- ISO medium
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Twenty animals, divided into four replicates (glass beaker; volume app. 50 mL) of five animals each were exposed to the test concentration or run as control for 48 hours in a static test.
The immobility or mortality of the Daphnia was determined by visual observation after 24 and 48 hours of exposure. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- app. 249 mg/l (as CacCO3)
- Test temperature:
- The test temperature was in the range of 21.0 – 21.3°C measured in the test vessels during the test. The additionally measured ambient temperature within the climate chamber was between 20.3 – 21.7°C.
- pH:
- The pH of the test solutions was not adjusted and did not vary by more than 1.5 units in any one test. The pH was in the range of 7.63 – 7.88 during the test.
- Dissolved oxygen:
- The dissolved oxygen concentration was in the range of 7.26– 8.32 mg/L during the test.
- Nominal and measured concentrations:
- Based on the results of the preliminary test, a single test concentration at 100 mg/L nominal concentration and one control group were tested in a limit test. Biological results and endpoints are based on the nominal concentration.
- Details on test conditions:
- Light-Dark Cycle: The light-dark cycle during the test was 16 hours light (artificial illumination) and 8 hours darkness.
- Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this 48-hour acute toxicity test on Daphnia magna, the obtained results showed that the test item Centella asiatica dry extract had no toxic effects on the mobility of the daphnids at the limit concentration of 100 mg/L.
All validity criteria were met. The results are based on the nominal test item concentration (100 mg/L) and were determined directly from the raw data.
The 48-h NOEC was determined to be 100 mg/L and the LOEC and the EC50 values were determined to be > 100 mg/L
Reference
Description of key information
The 48-h NOEC was determined to be 100 mg/L and the LOEC and the EC50 values were determined to be > 100 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
The study was regarded as “ reliable with restriction” (Klimish score 2), because the toxicity values were determined on the basis of the analytical measurement of the main components Madecassicoside and of the Asiaticoside, that are expected to be hydrolytically instable (see hydrolysis study).
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