Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 207-559-1 | CAS number: 480-96-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- 1980
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- significant methodological deficiencies
Data source
Reference
- Reference Type:
- publication
- Title:
- THE MUTAGENIC ACTION OF QUINDOXIN, CARBADOX, OLAQUINDOX AND SOME OTHER N-OXIDES ON BACTERIA AND YEAST
- Author:
- VOOGD CE, VAN DER STEL JJ, JACOBS JA
- Year:
- 1 980
- Bibliographic source:
- MUTAT RES 78:233-242,1980
Materials and methods
- Principles of method if other than guideline:
- The plate incorporation method and the fluctuation method were used to test the bacterial reverse mutation. In the plate incorporation method only two strains of bacteria instead of five strains were used. No strain with AT base pair at the primary reversion site was used. Bacteria were not exposed to the test substance in the presence of a metabolic activation system. The use of concurrent strain-specific positive and negative controls was not documented.
Fluctuation tests were carried using a Klebsiella pneumoniae mutant, requiring uracil and proline as growth factors, and Escherichia coli K12 Hfr Hayes. - GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 1-benzo-2-oxa-1,3-diazole oxide
- EC Number:
- 207-559-1
- EC Name:
- 1-benzo-2-oxa-1,3-diazole oxide
- Cas Number:
- 480-96-6
- Molecular formula:
- C6H4N2O2
- IUPAC Name:
- 2,1,3-benzoxadiazol-1-ium-1-olate
Constituent 1
- Specific details on test material used for the study:
- SOURCE: Bayer GmbH, Leverkusen (F.R.G.)
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Species / strain / cell type:
- bacteria, other: Klebsiella pneumoniae
- Additional strain / cell type characteristics:
- other: requiring uracil and proline as growth factors
- Metabolic activation:
- without
- Test concentrations with justification for top dose:
- plate incorporation method: Benzofuroxan concentration 1/0.5/0.2/0.1/0.05/0.02/0.01/0.005 mmole/L
fluctuation method: Benzofuroxan concentration in top agar 2/1/0.5/0.2/0.1 mmole/L - Vehicle / solvent:
- Benzofuroxan was dissolved in dimethylsulphoxide.
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- no
- True negative controls:
- no
- Positive controls:
- no
- Remarks:
- The use of concurrent strain-specific positive and negative controls was not documented.
- Details on test system and experimental conditions:
- PLATE-INCORPORATION TEST
The plate-incorporation test developed by Ames (Ames et al., 1975) was used with Salmonella typhimurium TA98 and TA100. These strains were kindly supplied by Professor Ames (Department of Biochemistry, University of California, Berkeley, CA 94720 BC 01, U.S.A.). No metabolic activation was applied. On each plate 3 ml of top agar, in which the compound under investigation was present, were added to 20 ml of the selection medium. The compounds were dissolved in dimethylsulphoxide.
FLUCTUATION TEST
Fluctuation tests (Luria and Delbrtick, 1943) were carried out as described in Voogd et al., 1977. As test organisms a Klebsiella pneumoniae mutant, requiring uracil and proline as growth factors, and Escherichia coli K12 Hfr Hayes were used. After incubation during 20 h at 37°C, the total number of streptomycin-resistant (including streptomycin-dependent) bacteria was determined by the pour-plate technique. The average spontaneous mutation rate of Klebsiella pneumoniae was 0.1676 × 10 -9 (S.D. 0.0304 × 10 -9) obtained from 26 independent experiments, and that of E. coli was 0.2393 × 10 -9 (S.D. 0.0596 × 10 -9) obtained from 23 experiments.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- without
- Genotoxicity:
- positive
- Vehicle controls validity:
- not valid
- Untreated negative controls validity:
- not valid
- Positive controls validity:
- not valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- without
- Genotoxicity:
- positive
- Vehicle controls validity:
- not valid
- Untreated negative controls validity:
- not valid
- Positive controls validity:
- not valid
- Species / strain:
- other: Klebsiella pneumonia
- Genotoxicity:
- positive
- Additional information on results:
- PLATE-INCORPORATION TEST
A weak mutagenic activity was observed with Salmonella typhimurium TA100 when benzofuroxan was present in the top agar at 1 mmole/L and with Salmonella typhimurium TA98 when benzofuroxan was present in the top agar at 0.5 mmole/L.
FLUCTUATION TEST
An increase in the mutation rate of Klebsiella pneumoniae was caused by benzofuroxan. In this test, benzofuroxan doubled the mutation rate at a concentration of 0.01 mmole/L.
Applicant's summary and conclusion
- Conclusions:
- Benzofuroxan may cause both base-pair substitutions and frame-shift mutations. However, this study is not reliable due to major methodological deficiencies. Therefore, the data are insufficient for assessment.
- Executive summary:
The plate incorporation method and the fluctuation method were used to test the bacterial reverse mutation. In the plate incorporation method Salmonella typhimurium TA98 and TA100 were used. No strain with AT base pair at the primary reversion site was used. Bacteria were not exposed to the test substance in the presence of a metabolic activation system. The use of concurrent strain-specific positive and negative controls was not documented.
Fluctuation tests were carried using a Klebsiella pneumoniae mutant, requiring uracil and proline as growth factors, and Escherichia coli K12 Hfr Hayes.
A weak mutagenic activity was observed with Salmonella typhimurium TA100 when benzofuroxan was present in the top agar at 1 mmole/L and with Salmonella typhimurium TA98 when benzofuroxan was present in the top agar at 0.5 mmole/L. An increase in the mutation rate of Klebsiella pneumoniae was caused by benzofuroxan. In this test, benzofuroxan doubled the mutation rate at a concentration of 0.01 mmole/L.
Benzofuroxan may cause both base-pair substitutions and frame-shift mutations. However, this study is not reliable due to major methodological deficiencies. Therefore, the data are insufficient for assessment.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.