5-(dithiolan-3-yl)valeric acid

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2017-02-07 - 2017-03-08

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

2004-04-13

Deviations:

yes

Remarks:

In Replicate 2 at test item cocentration 100 mg/L 6 daphnids were insertetd instead of 5. Impact on Study: None, since all daphnids were considered in the evaluation.

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

5-(dithiolan-3-yl)valeric acid

EC Number:

214-071-2

EC Name:

5-(dithiolan-3-yl)valeric acid

Cas Number:

1077-28-7

Molecular formula:

C8H14O2S2

IUPAC Name:

5-(1,2-dithiolan-3-yl)pentanoic acid

impurity 1

Reference substance name:

unknown

IUPAC Name:

unknown

impurity 2

Reference substance name:

Water

EC Number:

231-791-2

EC Name:

Water

Cas Number:

7732-18-5

Molecular formula:

H2O

IUPAC Name:

dihydrogen oxide

Test material form:

solid: bulk

Details on test material:

This composition is the usual techical grade of AlzChem AG. It will be used when test substance has this composition (or is very closed) or no specific information is available

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

All test item concentrations + control at test start and after 48 h from aged solutions.

Test solutions

Vehicle:

no

Details on test solutions:

Nominal concentrations were tested in the main test: 4.27, 9.39, 20.7, 45.5 and 100 mg/L and control.

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

other: Elendt M4 test medium

Remarks:

OECD 202, annex 3

Total exposure duration:

48 h

Post exposure observation period:

After 24 h and after 48 h immobilized daphnids were counted.
All daphnids not able to swim within 15 seconds after gentle agitation of the test vessel were considered to be immobilised.

Test conditions

Hardness:

140 - 250 mg/L (as CaCO3)
7.8 - 14°dH

Test temperature:

19.0 - 20.8 °C

pH:

6.66 - 7.95

Dissolved oxygen:

>= 8.6 mg/L

Nominal and measured concentrations:

Nomional: 4.27, 9.39, 20.7, 45.5 and 100 mg/L

Details on test conditions:

Light: 16 h photoperiod; 8 h darknes daily
Feeding: None
Test vessels: 4 ~ 50 mL glass bottles per concentration, each filled with ~ 50 ml, one additional replicate for measurements w/o test organisms
Loading: ~ 10 mL of test solution for each animal
Aeration: none
Number of animals: 20 per concentration in 4 replicates of 5

Reference substance (positive control):

yes

Remarks:

potassium dichromate (K2Cr2O7)

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 15.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

ca. 4.27 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

ca. 32.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

ca. 9.39 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Reported statistics and error estimates:

The 24 h and 48 h EC50 are the estimated concentrations where 50 % of the daphnids were
immobilised after 24 and 48 hours, respectively.
The values for EC50 were determined by probit analysis using linear max. likelihood regression.
The evaluation of data was performed by ToxRat Professional 3.2.1.
The NOEC was established based on the highest concentration at which the immobilisation is not
higher than the allowed control immobilisation (􀂔 10 % immobilisation).

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

According to the results of the test, the EC50 (48 h) was determined to be
15.1 mg/L (nominal) respectively 10.2 mg/L (actual). The corresponding
NOEC (48 h) was 4.27 mg/L (nominal) respectively 3.71 mg/L (actual).

Executive summary:

According to the results of the test, the EC50 (48 h) was determined to be

15.1 mg/L (nominal) respectively 10.2 mg/L (actual). The corresponding

NOEC (48 h) was 4.27 mg/L (nominal) respectively 3.71 mg/L (actual).