6,6',6''-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoic acid, compound with 2,2',2''-nitrilotriethanol (1:3)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1983/12/19 to 1983/12/23

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non GLP study. Guideline from 1981.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

no

Analytical monitoring:

yes

Details on sampling:

- Sampling method: Samples for analysis were taken at 0 h and 96h exposure.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Calculated anounts of stock solution to produce the desired test concentrations were given into the water and honogeneously distributed.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow trout
- Source: P. Hohler CH-4314 Zeiningen
- Age at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD): 57 mm (49 - 60 mn)
- Weight at study initiation (mean and range, SD): 1.6 g (1.0 - 1.9 g)
- Feeding during test: none

ACCLIMATION
- Acclimation period: 27 days

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

183 mg CaCO3/L

Test temperature:

14 °C

pH:

8 to 8.3

Dissolved oxygen:

80 to 101 %

Nominal and measured concentrations:

100, 180, 320, 580, 1000 mg/L (nominal)
99.7, 176.7, 310.1, 541.0, 972.5 mg/L (measured)

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass aquaria of 20 liters filled with 15 liters (36/22/25 cm)
- Aeration: Gentle aeration after 24 h exposure
- No. of organisms per vessel: 5 fishes per aquarium
- No. of vessels per concentration (replicates): two replicates
- No. of vessels per control (replicates): two replicates
- Biomass loading rate: 0.5 g/L

TEST MEDIUM / WATER PARAMETERS
- in accordance to the guideline

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours daily
- Light intensity: Fluorescenet light

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
mortality, visible abnormalities were measured after 24, 48, 72 and 96 hours

TEST CONCENTRATIONS
- Spacing factor for test concentrations: approx. 1.8

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

>= 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: no behaviour abnormalities were observed
- Mortality of control: no mortality
- Other adverse effects control: no effects observed

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study meets generally accepted scientific principles, acceptable for assessment

Qualifier:

equivalent or similar to guideline

Guideline:

other: APHA method (1980)

GLP compliance:

not specified

Analytical monitoring:

yes

Details on sampling:

- concentrations were analysed by gas-liquid chromatography (GLC)

Vehicle:

no

Test organisms (species):

Pimephales promelas

Details on test organisms:

Test species: Fathead minnow (Pimephales promelas)      
- age (d): 30       
- mean body length (mm): 18.1 ± 0.788      
- mean weight (g): 0.083 ± 0.0083      
- loading (g/l): 0.1153

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

25.7 ± 0.38 °C

pH:

7.8 ± 0.07

Dissolved oxygen:

7.3 mg/l

Nominal and measured concentrations:

Nominal test concentrations (g/l): 0, 7.30, 11.2, 17.3, 26.6, and 40.9
test substance recovery by GLC: 107.2 ± 3.8% (n = 4)

Details on test conditions:

TEST SYSTEM
- Test vessel: 20 fish/concentration
- tank volume (l): 1.0 (additions 14.4 v/d)
- pH: the pH of the stock solution (90.4 mg/l) was adjusted to that of lake water using HCl.

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

11 800 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: pH was adjusted to that of lake water

Reported statistics and error estimates:

The estimated LC/EC50 values with corresponding 95% confidence intervals were calculated using the corrected average of the analyzed tank concentrations and the Trimmed Spearman-Kaerber method (Hamilton et al., 1977).

Sublethal observations / clinical signs:

Effect values (related to measured concentrations): 96-h EC50 (g/l): 11.8 (10.6-13.0)

Mortalities

Nominal conc (g/l)	Mean measured (corr.)	No. of fish at start	Dead fish after
			24h	48h	72h	96h
Control	< 0.02	20	0	0	0	0
7.30	6.14	20	0	0	0	0
11.2	9.86	20	1	3	4	4
17.3	15.4	20	15	17	18	18
26.6	24.4	20	20	20	20	20
40.9	38.1	20	20	20	20	20

Physiological/behavioral effects 
Affected fish showed loss of schooling behaviour, hyperactivity, dark coloration, increased respiration and loss of equilibrium prior to death.

 

Validity criteria fulfilled:

yes

Description of key information

The compound consists of a triazine part and an amine part. Under environmental conditions it is present in its dissociated form. Therefore, separate assessments were conducted for the triazine and the amine part, respectively.  

CAS 80584 -91 -4: There is a high probability that the compound is not acutely harmful to fish.  

CAS 102 -71 -6: There is a high probability that the compound is not acutely harmful to fish.

Additional information

The compound consists of a triazine part and an amine part. Under environmental conditions it is present in its dissociated form. Therefore, separate assessments were conducted for the triazine and the amine part, respectively.

CAS 80584-91-4

The acute toxicity to fish was assessed in a key and supporting study. In the key study rainbow trout and in the supporting study zebra fish, were exposed under static conditions to nominal concentrations of 100, 180, 320, 580 and 1000 mg/L. In both studies the LC50 was determined to be > 1000 mg/L.

CAS 102-71-6

To determine the toxicity of 2,2’,2’’-nitrilotriethanol to fish, a non-GLP study similar to APHA method using Pimephales promelas and a flow-through procedure was performed by Geiger et al. (1990). The calculated LC50 value after 96 hours in this study was 11800 mg/l related to the nominal concentrations (confirmed by concentration control analytics). Hence, the chemical is considered to be acutely not harmful for fish. Other data which are available for 2,2’,2’’-nitrilotriethanol are disregarded and shout not be used for risk assessment purposes mainly due to the insufficient exposure duration.