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EC number: 204-354-9 | CAS number: 119-84-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 01.07.98-08.10.98
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 998
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Coumarin
- EC Number:
- 202-086-7
- EC Name:
- Coumarin
- Cas Number:
- 91-64-5
- Molecular formula:
- C9H6O2
- IUPAC Name:
- 2H-chromen-2-one
- Test material form:
- solid: crystalline
- Details on test material:
- Solution A: KH2PO4 8.5g K2HPO4 21.75g Na2HPO4.2H2O 33.4g NH4Cl 0.5g
Solution B:CaCl2 27.5g
Dissolved in water and made up to 1 litre
Solution C:MgSO4.7H2O 22.5g
Dissolved in water and made up to 1 litre
FeCl3.6H2O 0.25g HCl Conc. One drop
SolutionD:
Dissolved in water and made up to 1 litre
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- Fresh acticated sludge from a biological waste water treatment plant treating predominantly domestic sewage(City of Geneva,Aire) was used.
- Duration of test (contact time):
- ca. 28 d
Initial test substance concentration
- Initial conc.:
- ca. 100 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- Test condition
-Composition of medium: Made in accordance with OECD 301F guideline
-Solubilising agent: none
-Test temperature: 22±1℃
-PH: 7.4±0.2
-PH adjusted: yes
-Suspended solids concentration: 30 mg/l
Test system
-Culturing apparatus: Flasks of the SAPPOMAT D12, made by J.M. VOITH Gmbh, D-7920 Heidenheim
-Measuring equipment: The respirometer used during this study is a SAPPOMAT D12, made by J.M. VOITH Gmbh, D-7920 Heidenheim
Control and blank system
-Inoculum blank: yes( mineral medium+ inoculum)
-Abiotic sterile control: yes (mineral medium + test chemical (100mg/l)+Reference substance (100mg/l)
-Toxicity control: Yes (mineral medium + test chemical (100mg/l)+Reference substance (100mg/l+ inoculum
A measured volume of inoculated mineral medium, containing a known concentration of test substance (100mg/l) as nominal sole source of organic carbon, is stirred in a closed flask at a constant temperature (±1℃) for up to 28 days. The consumption of oxygen is determined by measuring the quantity of oxygen (produced electrolytically) required to maintain constant the gas volume in the respirometer flask. Evolved carbon dioxide is absorbed in soda lime pellets. The test chemical (corrected for uptake by blank inoculums, run in parallel) is expressed as a percentage of ThOD (Theoretical Oxygen Demand, calculated from the elemental composition, assuming that carbon is oxidized to carbon dioxide and hydrogen to water).
Reference substance
- Reference substance:
- benzoic acid, sodium salt
- Remarks:
- Purity: min 99.0%
Results and discussion
- Preliminary study:
- Not performed
- Test performance:
- No unusual observations affecting results performed
% Degradationopen allclose all
- Parameter:
- % degradation (O2 consumption)
- Value:
- ca. 82
- Sampling time:
- 7 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- ca. 85
- Sampling time:
- 12 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- ca. 87
- Sampling time:
- 14 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- ca. 89
- Sampling time:
- 21 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- ca. 90
- Sampling time:
- 28 d
- Details on results:
- See "Any other information on results incl. tables
BOD5 / COD results
- Results with reference substance:
- Degradation of sodium benzoate exceeds 40% after 7days and 65% after14days: the activity of the inoculum is thus verified and the test is considered as valid.
Any other information on results incl. tables
Test substance: Coumarin Pure Crystals
B=(B1 +B2)/2; D1=100*(C1 -B)/ThOD*[S]; D2=100*(C2 -B)/ThOD*[S]; D=(D1 +D2)/2. [S]:Initial test substance concentration(mg/L).
|
Days: |
2 |
7 |
12 |
14 |
21 |
28 |
|
BOD Sludge |
1rst flask B1 2nd flask B2 mean B |
8.0 |
10.0 |
14.0 |
14.0 |
16.0 |
16.0 |
|
8.0 |
14.0 |
20.0 |
20.0 |
22.0 |
22.0 |
|||
8.0 |
12.0 |
17.0 |
17.0 |
19.0 |
19.0 |
|||
BOD Test Subst. |
1rst flask C1 2nd flask C2 1rst fl.corr C1-B 2nd fl.corr C2-B |
104.0 |
186.0 |
198.6 |
201.4 |
206.3 |
210.1 |
|
89.2 |
179.1 |
190.7 |
193.5 |
200.3 |
203.1 |
|||
96.0 |
174.0 |
181.6 |
184.4 |
197.3 |
191.1 |
|||
81.2 |
167.1 |
173.7 |
176.5 |
181.3 |
184.1 |
|||
% degr |
1rst flask D1 2nd flask D2 mean D |
46 |
84 |
87 |
89 |
90 |
92 |
|
39 |
80 |
84 |
85 |
87 |
89 |
|||
43 |
82 |
85 |
87 |
89 |
90 |
Test substance: SODIUM BENZOATE
B=(B1 +B2)/2; D1=100*(C1 -B)/ThOD*[S]; D2=100*(C2 -B)/ThOD*[S]; D=(D1 +D2)/2. [S]:Initial test substance concentration(mg/L).
|
Days: |
5 |
7 |
10 |
14 |
21 |
28 |
|
BOD Sludge |
1rst flask B1 2nd flask B2 mean B |
10.0 |
10.0 |
14.0 |
14.0 |
16.0 |
16.0 |
|
12.0 |
14.0 |
18.0 |
20.0 |
22.0 |
22.0 |
|||
11.0 |
12.0 |
16.0 |
17.0 |
19.0 |
19.0 |
|||
BOD Refer Subst. |
1rst flask A1 2nd flask A2 1rst fl.corr A1-B 2nd fl.corr A2-B |
130.0 |
142.0 |
156.0 |
168.0 |
176.0 |
180.0 |
|
128.0 |
140.0 |
154.0 |
164.0 |
170.0 |
172.0 |
|||
119.0 |
130.0 |
140.0 |
151.0 |
157.0 |
161.0 |
|||
117.0 |
128.0 |
138.0 |
147.0 |
151.0 |
153.0 |
|||
% degr |
1rst flask D1 2nd flask D2 mean D |
71 |
78 |
84 |
91 |
94 |
97 |
|
70 |
77 |
83 |
88 |
91 |
92 |
|||
71 |
77 |
83 |
89 |
92 |
94 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- Degradation of sodium benzoate exceeds 40% after 7days and 65% after14days: the activity of the inoculum is thus verified and the test is considered as valid.
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Coumarin pure crystal undergoes 90% biodegradation after 28 days in the test conditions. Biodegradation starts on day 2 and reaches 85% at the end of the 10-day window (days 2 to 12).
Thus coumarin pure crystal should be regarded as readily biodegradation according to this test.
The curves obtained with the reference substance alone and with coumarin pure crystals + reference substance show no toxic effect of coumarin pure crystal to the micro-organisms at the test concentration. - Executive summary:
The Read Biodegradability of COUMARIN PURE CRYSTALS was determined by the Manometric Respirometry Test according to the OECD Guidelines for Testing of Chemicals, Method No. 301 F.
COUMARIN PURE CRYSTALS undergoes 90% biodegradation after 28days in the test conditions. Biodegradation stars on day 2 and reaches 85 % at the end of the 10-day window(days 2-12).
Thus, COUMARIN PURE CRYSTALS should be regarded as readily biodegradable according to this test.
At the concentration used in the test (100mg/l). COUMARIN PURE CRYSTALS is not inhibitory to the micro-organisms.
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