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EC number: 221-698-5 | CAS number: 3195-78-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Specific investigations: other studies
Administrative data
- Endpoint:
- specific investigations: other studies
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 993
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The genetic principle: loss of heterozygosity for the wild-type reporter gene w+, an event predominantly resulting from homologous interchromosomal mitotic recombination between the two X chromosomes of female genotypes.
- GLP compliance:
- no
- Type of method:
- in vivo
- Endpoint addressed:
- other: DNA modifications
Test material
- Reference substance name:
- N-methyl-N-vinylacetamide
- EC Number:
- 221-698-5
- EC Name:
- N-methyl-N-vinylacetamide
- Cas Number:
- 3195-78-6
- Molecular formula:
- C5H9NO
- IUPAC Name:
- N-ethenyl-N-methylacetamide
Constituent 1
Test animals
- Species:
- other: Drosophila (Cross C-1: y (yellow) females X w (white) males
- Details on test animals or test system and environmental conditions:
- Strains y and w contain chromosomes from two laboratory stocks used when these mutants were isolated in induction experiments with N-ethyl-N-nitrosourea
Administration / exposure
- Route of administration:
- other: acute feeding and inhalation
- Vehicle:
- other: 200 µg/L enthanol (inhalation) or 3% ethanol, 1% Tween 80 (acute feeding)
- Details on exposure:
- Acute feeding:
Lavea were collected by washing them out of 58-72 h old mass cultures (20-30 pairs per bottle, 24 h egg-laying period) with an aqueous solution of 15% sucrose. The sucrose solution containing the larvae was filtered through nylon gauze, washed with 0.7% NaCl solution and 200-400 mg larvae was resuspended in 5 ml 0.7% NaCl solution in a test tube. To this solution the test agent, dissolved in a solvent, was added to yield the desired concentration. Seven minutes later a second aliquot of the test chemical was added. After 15 min of total exposure, the test chemical was removed by filtration on a nylon gauze, the larvae washed three times with 0.7% NaCl solution and placed in bottles on standard food. Addition of 0.5-1 ml NaCl solution was helpful to reach a more uniform distribution othe larvae over the surface.
Inhalation:
Flies (50 pairs per bottle) were put on a thin layer ( about 1 cm) of standard food in 600 ml bottles having screw caps and females were allowed to oviposit for 24 h. The parental flies were discarded, the culture kept at 25°C for antother 48 h and then 0.1 ml ethanol containing the test chemical was added to yield the desired doses. These treatment units were kept tightly sealed for 17 h and incubated at 25°C. Then the larvae were removed by using a 15% sucrose solution, washed and placed in bottles with standard food. Thus at the beginning of the inhalation treatment larvae were 28-52 h old (48-72 h old cultures). - Analytical verification of doses or concentrations:
- no
Doses / concentrationsopen allclose all
- Dose / conc.:
- 1 000 ppm
- Remarks:
- inhalation
- Dose / conc.:
- 2 000 ppm
- Remarks:
- inhalation
- Dose / conc.:
- 40 other: mM
- Remarks:
- acute feeding
- Dose / conc.:
- 80 other: mM
- Remarks:
- acute feeding
- Dose / conc.:
- 160 other: mM
- Remarks:
- acute feeding
- Details on study design:
- Sample size:
500 - 750 eyes tested
Examinations
- Examinations:
- Microscopic analysis of eyes:
Newly hatched females was transfered to fresh medium and scored 1-5 days later. The scoring of etherized flies was carried out in a liquid consisting of 90 parts ethanolm 1 part Tween 80 and 9 parts water. The eyes of the adult females were inspected for mosaic light spots under a dossecting microscope at a magnification of 50-75 x. Spots separated from each other by at least four non-mutated ommatidia were counted as independent events.
Data analysis:
For data analysis, a distinction was made as follows:
small spots (size classes: 1-2 and 3-4)
large spots (all clones > 4 ommatidia)
total spots
For an indirect estimation of genotoxic effectiveness in eye disc cells, the frequency of clones per 10E4 cells was calculated according to the formula f = 2 nm/ NC, where m= mean clone size, n = number or mosaic spots, 2 = correction factor, C is 800 (ommatidia) and N= number of eyes analysed.
Test response were classified into three categories:
Positive ++
A strong recombinagenic response and a dose-response relation was found.
Weakly positive +w
The clone frequency was significantly enhanced compared with both concomitant and pooled controls, but no more than about a doubling of the spontaneous spot frequency is associated with signs of toxicity meaning that a dose-response relationship could not be established.
Negative -
No effect under all conditions of test - Positive control:
- no data
Results and discussion
- Details on results:
- Please refer to any other information on results incl. tables
Any other information on results incl. tables
Results of the w/w+ eye mosaic test after inhalative treatment and acute feeding
Inhalation |
|||||||
concentration |
Eyes tested |
Spots per 100 eyes |
Average clone size |
Clones per 10E4 cells |
Activity |
||
S |
L |
T |
|||||
0 ppm |
500 |
2.80 |
0.40 |
3.2 |
3.4 |
2.7 |
|
1000 ppm |
750 |
2.00 |
0.67 |
2.7 |
2.9 |
1.9 |
- |
2000 ppm |
750# |
3.07 |
0.93 |
4.0 |
5.1 |
5.1 |
- |
Acute feeding |
|||||||
concentration |
Eyes tested |
Spots per 100 eyes |
Average clone size |
Clones per 10E4 cells |
Activity |
||
S |
L |
T |
|||||
0 mM |
500 |
3.60 |
0.40 |
4.0 |
2.3 |
2.3 |
|
40 mM |
500 |
5.20 |
0.80 |
6.0 |
3.6 |
5.4 |
i |
80 mM |
500 |
7.00 |
0.80 |
7.8 |
3.3 |
6.4 |
+w |
160 mM |
500 |
5.40 |
1.00 |
6.4 |
3.3 |
5.3 |
i |
#indicated reduced survival in relation to the control
S small spots, clone size 1-4 ommatidia
L large spots, clone size > 4 ommatidia
T, total spots
- inactive (for more details please refer to chapter examinations)
i inconclusive (for more details please refer to chapter examinations)
+w weakly positive (for more details please refer to chapter examinations)
Results and conclusion
Under the conditions of the recent study inhalative treatment indicated no increased activity compared to the control group. Only at a concentration of 80 mM a weakly positive response were indicated after acute feeding with the test item. However, the result was associated with signs of toxicity meaning that a dose-response relationship could not be established and thus, in conclusion the test item was considered to have a relatively low DNA reactivity.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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