Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 229-043-5 | CAS number: 6407-78-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from peer reviewed journal
Data source
Reference
- Reference Type:
- publication
- Title:
- Final Report on the Safety Assessment of Phenyl Methyl Pyrazolone
- Author:
- Cosmetic Ingredient Expert Review Panel
- Year:
- 1 992
- Bibliographic source:
- JOURNAL OF THE AMERICAN COLLEGE OF TOXICOLOGY Volume 11, Number 4,1992
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- Skin sensitization study of3-Methyl-1-Phenyl-5-Pyrazolone (CAS no: 89-25-8) was conducted on 20 guinea pigs to assess its skin sensitization potential.
- GLP compliance:
- not specified
- Type of study:
- guinea pig maximisation test
- Justification for non-LLNA method:
- not specified
Test material
- Reference substance name:
- 3-methyl-1-phenyl-5-pyrazolone
- EC Number:
- 201-891-0
- EC Name:
- 3-methyl-1-phenyl-5-pyrazolone
- Cas Number:
- 89-25-8
- Molecular formula:
- C10H10N2O
- IUPAC Name:
- 1-Phenyl-3-methyl-5-pyrazolone
- Test material form:
- solid
- Details on test material:
- - Name of test material (as cited in study report): Phenyl methyl pyrazolone (PMP)
- Molecular formula :C10H10N2O
- Molecular weight :174.2g/mole
- Substance type:organic
- Physical state: solid
Constituent 1
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Phenyl methyl pyrazolone (PMP)
- Molecular formula :C10H10N2O
- Molecular weight :174.2g/mole
- Substance type:organic
- Physical state: solid
In vivo test system
Test animals
- Species:
- guinea pig
- Strain:
- other: albino
- Sex:
- male/female
- Details on test animals and environmental conditions:
- No data available
Study design: in vivo (non-LLNA)
Induction
- Route:
- intradermal and epicutaneous
- Vehicle:
- water
- Concentration / amount:
- Epicutaneous concentration: 0.50 ml of PMP, a 0.5% in (w/w) water.
Intradermal concentration: 0.1ml3 freund’s complete adjuvant diluted to 50% with sterile isotonic saline. - Day(s)/duration:
- 3 weeks
- Adequacy of induction:
- not specified
Challenge
- No.:
- #1
- Route:
- epicutaneous, occlusive
- Vehicle:
- water
- Concentration / amount:
- 0.5 ml
- Day(s)/duration:
- 48 hours
- Adequacy of challenge:
- not specified
- No. of animals per dose:
- Total :20
Male :10
Female:10 - Details on study design:
- Details on study design
RANGE FINDING TESTS:
MAIN STUDY
A. INDUCTION EXPOSURE
- No. of exposures:10
- Exposure period:1hr
- Test groups:20
- Control group:No data available
- Site: clipped skin of left scapular region
- Frequency of applications:3 times in week
- Duration:3 weeks
- Concentrations: Epicutaneous concentration: 0.50 ml of PMP, a 0.5% in (w/w) water.
Intradermal concentration: 0.1ml3 freund’s complete adjuvant diluted to 50% with sterile isotonic saline.
B. CHALLENGE EXPOSURE
- No. of exposures: one
- Day(s) of challenge:After 12 days rest period
- Exposure period:1hr
- Test groups:20
- Control group:
- Site: clipped skin of left flank of abdomen
- Concentrations:0.5 ml
- Evaluation (hr after challenge):1, 6, 24, 48 hrs.
OTHER: After removal of patch, the test site was rinsed with water. - Challenge controls:
- No data available
- Positive control substance(s):
- not specified
Results and discussion
In vivo (non-LLNA)
Results
- Reading:
- 1st reading
- Hours after challenge:
- 48
- Group:
- test chemical
- Dose level:
- 0.5 ml
- No. with + reactions:
- 0
- Total no. in group:
- 20
- Clinical observations:
- Skin sensitization reactions were not observed
- Remarks on result:
- no indication of skin sensitisation
Applicant's summary and conclusion
- Interpretation of results:
- other: Not Sensitizing
- Conclusions:
- The test material 3-Methyl-1-Phenyl-5-Pyrazolonewas (CAS No:-89-25-8) was considered to be not sensitizing to the skin of albino guinea pigs under occlusive condition.
- Executive summary:
Skin sensitization study of 3-Methyl-1-Phenyl-5-Pyrazolone(CAS no: 89-25-8) was conducted on 20 guinea pigs to assess its skin sensitization potential.
In induction phase, 0.5ml of a 0.5% aqueous 3-methyl-1-phenyl-5-pyrazolone solution was applied dermally onclipped skin of left scapular regionfor 1hr under occlusive condition. This procedure was repeated 3 times in week for total 10 applications. After removal of patch the test site was rinsed with water. During the study, the guinea pigs received 2 intradermal injections of 0.1ml3freund’s complete adjuvant diluted to 50% with sterile isotonic saline near the test site.
After 12 days rest period, animals were challenged at concentration of 0.50ml on clipped skin of left flank of abdomen using occlusive patch for 1 hour. After removal of patch, skin site was rinsed and observations were made after 1, 6, 24, 48 hrs.
None of the animal produced any skin reactions at challenge concentration .Hence, 3-Methyl-1-Phenyl-5-Pyrazolonewas (CAS No:-89-25-8) was considered to be not sensitizing to the skin of albino guinea pigs.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.